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Human Transmembrane protein 119(TMEM119) ELISA kit

  • 中文名稱:
    人跨膜蛋白119(TMEM119)酶聯免疫試劑盒
  • 貨號:
    CSB-EL023686HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人跨膜蛋白119(TMEM119)酶聯免疫試劑盒(CSB-EL023686HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TMEM119含量。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,適用于體外培養的小膠質細胞模型中蛋白表達水平檢測、動物腦組織炎癥狀態評估,或探索外周血中TMEM119與神經系統疾病潛在關聯的科研場景。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    TMEM119 ELISA Kit; PSEC0199 ELISA Kit; UNQ731/PRO1415 ELISA Kit; Transmembrane protein 119 ELISA Kit; Osteoblast induction factor ELISA Kit; OBIF ELISA Kit
  • 縮寫:
    TMEM119
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human TMEM119 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 107
    Range % 103-110
    1:2 Average % 88
    Range % 84-92
    1:4 Average % 107
    Range % 103-110
    1:8 Average % 90
    Range % 87-93
  • 回收率:
    The recovery of human TMEM119 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 100-108
    EDTA plasma (n=4) 88 84-93
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 1.805 1.831 1.818 1.691
    2000 1.501 1.600 1.551 1.424
    1000 1.282 1.198 1.240 1.113
    500 1.003 1.068 1.036 0.909
    250 0.704 0.712 0.708 0.581
    125 0.471 0.498 0.485 0.358
    62.5 0.356 0.362 0.359 0.232
    0 0.126 0.128 0.127  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Plays an important role in bone formation and normal bone mineralization. Promotes the differentiation of myoblasts into osteoblasts. May induce the commitment and differentiation of myoblasts into osteoblasts through an enhancement of BMP2 production and interaction with the BMP-RUNX2 pathway. Upregulates the expression of ATF4, a transcription factor which plays a central role in osteoblast differentiation. Essential for normal spermatogenesis and late testicular differentiation.
  • 基因功能參考文獻:
    1. TMEM119 expression in osteosarcoma tissues is positively correlated with cell cycle, apoptosis, metastasis and TGF-beta signaling. PMID: 28496199
    2. TMEM119 serves as a reliable microglial marker that discriminates resident microglia from blood-derived macrophages in the human brain PMID: 26250788
    3. qPCR showed that TMEM119 expression was highly enriched in temporal lobe CD11b+ cells over unpurified whole brain, and barely detectable in peripheral blood leukocytes. PMID: 26884166
  • 亞細胞定位:
    Cell membrane; Single-pass type I membrane protein. Cytoplasm. Endoplasmic reticulum membrane.
  • 組織特異性:
    Expressed in brain microglia (at protein level). Elevated expression levels seen in the brain of patients with Alzheimer disease. Expressed by osteoblast-like cells in bone tissues and follicular dendritic cells in lymphoid tissues.
  • 數據庫鏈接:

    HGNC: 27884

    KEGG: hsa:338773

    UniGene: Hs.449718



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