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Human Transforming growth factor-beta-induced protein ig-h3 (TGFBI/BIGH3) ELISA kit

  • 中文名稱:
    人轉化生長因子-β誘導蛋白IG - H3(TGFBI/BIGH3)酶聯免疫試劑盒
  • 貨號:
    CSB-E16665h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人轉化生長因子-β誘導蛋白IG - H3(TGFBI/BIGH3)酶聯免疫試劑盒(CSB-E16665h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的TGFBI含量。TGFBI 是重要靶點。它在細胞生長、分化等生理過程有參與,其異常表達與腫瘤、眼部疾病等多種病理情況相關。研究機制主要聚焦于其信號通路,探索其與疾病發生、發展聯系,以尋找潛在治療策略,為開發新療法提供方向。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,靈敏度為19.5 pg/mL。適用于基礎科研中探究該蛋白在纖維化疾病模型中的動態表達、腫瘤微環境基質重構研究,或用于評估藥物干預對TGF-β通路下游效應分子的調控作用,為細胞信號轉導、病理機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    RGD containing collagen associated protein ELISA Kit; AI181842 ELISA Kit; AI747162 ELISA Kit; Beta ig ELISA Kit; Beta ig h3 ELISA Kit; Beta ig-h3 ELISA Kit; BGH3_HUMAN ELISA Kit; Big h3 ELISA Kit; BIGH3 ELISA Kit; CDB1 ELISA Kit; CDG2 ELISA Kit; CDGG1 ELISA Kit; CSD ELISA Kit; CSD1 ELISA Kit; CSD2 ELISA Kit; CSD3 ELISA Kit; EBMD ELISA Kit; Kerato epithelin ELISA Kit; Kerato-epithelin ELISA Kit; LCD1 ELISA Kit; MGC150270 ELISA Kit; RGD CAP ELISA Kit; RGD-CAP ELISA Kit; RGD-containing collagen-associated protein ELISA Kit; TGFBI ELISA Kit; TGFBI transforming growth factor, beta induced, 68kDa ELISA Kit; Transforming growth factor beta induced protein ig h3 ELISA Kit; Transforming growth factor-beta-induced protein ig-h3 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 pg/mL-5000 pg/mL
  • 靈敏度:
    19.5 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    636.229

    580.524

    668.618

    598.802

    656.173

    586.342

    SD

    0.046

    0.049

    0.039

    0.059

    0.068

    0.058

    CV(%)

    4.224

    4.832

    3.448

    5.679

    6.099

    5.675

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human TGFBI/BIGH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:200

    Average %

    106

    Range %

    94-110

    1:400

    Average %

    100

    Range %

    93-103

    1:800

    Average %

    94

    Range %

    81-98

    1:1600

    Average %

    104

    Range %

    95-108

  • 回收率:

    The recovery of human TGFBI/BIGH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    85-95

    EDTA plasma (n=4)

    99

    94-107

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.877

    2.762

    2.820

    2.722

    2500

    2.264

    2.333

    2.299

    2.301

    1250

    1.756

    1.684

    1.720

    1.622

    625

    1.099

    1.157

    1.128

    1.030

    312

    0.573

    0.585

    0.579

    0.381

    156

    0.299

    0.304

    0.302

    0.204

    78

    0.172

    0.168

    0.170

    0.072

    0

    0.096

    0.100

    0.098

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展:
    TGFBI是一種編碼外泌蛋白的基因,它在細胞外基質的形成和維護中扮演著重要角色。最近的研究表明,TGFBI可能與多種疾病的發生和發展有關,如角膜疾病、腫瘤和心血管疾病等.
  • 功能:
    Plays a role in cell adhesion. May play a role in cell-collagen interactions.
  • 基因功能參考文獻:
    1. These results suggest that suppression of osteoblast differentiation by BIGH3 is one of the mechanisms that enhance osteolytic lesions in RCC bone metastasis, and raise the possibilty that treatments that increase bone formation may improve therapy outcomes. PMID: 29190493
    2. High TGFBI expression was significantly associated with poor prognosis and cancer progression. Downregulation of TGFBI in the cocultured gastric fibroblasts inhibited the invasion and migration abilities of the GC cells. PMID: 30260476
    3. Expression array analysis identified TGFBI as a key gene for the acquisition of malignant phenotype of pancreatic ductal adenocarcinoma (PDAC), and the expression of TGFBI was associated with poor prognosis in patients with PDAC. PMID: 30156359
    4. the genetic screening of TGFBI mutations must be incorporated into the pre-operative screening procedures to prevent exacerbation and recurrence, which eventually could lead to the need for a corneal transplant. PMID: 29192679
    5. Structural and functional implications of TGFBIp mutations in corneal dystrophies have been reported. PMID: 28988748
    6. Induction of TGFBI promotes prostate cancer growth and metastasis and can be caused by dysregulation or therapeutic inhibition of androgen receptor signaling. PMID: 28811384
    7. betaig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process. PMID: 28292713
    8. This is the first molecular analysis of TGFBI and CHST6 in Turkish patients with different types of corneal dystrophies. PMID: 27829782
    9. The more structurally organized A546T and V624M mutations in FAS1-4 domain of TGFBI had reduced deamidation rates, suggesting that a folded and stable FAS1-4 domain precludes Asn622 deamidation. PMID: 29140698
    10. A novel heterozygous TGFBI mutation was identified in the proband, c.1673T>G in exon 12 that leads to (Leu558Arg) change at the protein level. PMID: 27028151
    11. TGFBI and periostin act similarly in the heart in affecting fibrosis and disease responsiveness. PMID: 28750100
    12. The p.(Val113Ile) mutation results in an alteration of the atypical LCD phenotype associated with the p.(Leu558Pro) mutation. This represents only the second report of the alteration of the phenotype of a TGFBI dystrophy by a second, non-homozygous pathogenic mutation, and thus provides insight into the phenotype-genotype correlation of the TGFBI dystrophies. PMID: 27402970
    13. Our findings demonstrate that granular corneal dystrophy type 1 may present with a vortex pattern of anterior stromal deposits. Although this pattern of dystrophic deposits is not recognized by clinicians as a typical phenotype of GCD1, it is consistent with the production of the majority of the TGFBI protein by the corneal epithelium. PMID: 28060069
    14. The right cornea showed a higher number of lattice lines than the left cornea. Molecular analysis involving bidirectional complete sequencing demonstrated a novel T621P mutation in exon 14 of TGFBI. PMID: 27163623
    15. LASIK surgery of granular corneal dystrophy type 2 patients leads to accumulation and differential proteolytic processing TGFBI protein. PMID: 26864644
    16. High TGFBI expression in Esophageal squamous cell carcinoma is associated with hematogenous recurrence. PMID: 25448803
    17. Vicenin-2 and scolymoside could be a potential therapeutic agent for treatment of various severe vascular inflammatory diseases via inhibition of the TGFBIp signaling pathway. PMID: 26084452
    18. We observed that knockdown of Cul4A was associated with increased sensitivity to gemcitabine through upregulation of TGFBI in lung cancer cells. PMID: 26503734
    19. Data suggest that periostin and TGFBI (transforming growth factor-beta induced protein) can be considered paralogs in some ways: structural homology, phylogeny, and functions in extracellular matrix formation/cell adhesion/cellular immunity. [REVIEW] PMID: 26288337
    20. No mutations were detected in the TGFBI gene in Korean patients with macular corneal dystrophy. PMID: 26604660
    21. TGFBIp functions as a pro-lymphangiogenic factor.TGFBI is a lymphangiogenic factor contributing to tumor dissemination PMID: 25772247
    22. Studies reveal that growth hormone (GH)increases TGFBI expression in a mouse and rat models of diabetic nephropathy (DN) which correlate with clinical results, and suggest that this mechanism can explain the podocytes depletion in DN. PMID: 25740786
    23. the functional role of H3K4me in TGFbeta1-mediated TGFBIp and ECM gene expression in corneal fibroblasts PMID: 26553048
    24. 19 novel TGFB1 regulatory region and exon 1 alleles are presented: p018-p036. p018, p019, and p020 are formed by novel combinations of known polymorphic positions. Another 16 novel alleles are shown to carry additional known and unknown SNPs. PMID: 25808355
    25. The p. Arg555Trp mutation of the TGFBI gene was associated with Thiel-Behnke corneal dystrophy, which revealed a novel phenotype-genotype correlation within the mutational spectrum of phenotypically diverse corneal dystrophies. PMID: 26464103
    26. that locally generated TGFBIp at either wounds or tumor sites may contribute to differentiation and angiogenic function of endothelial progenitor cells by augmenting the recruitment of endothelial progenitor cells PMID: 25786978
    27. TGFBI functions as a promoter of certain gastrointestinal tract cancers. PMID: 25889002
    28. The authors present a unilateral variant of late-onset LCD with a heterozygous Pro501Thr mutation in the TGFBI gene. PMID: 23884333
    29. insights on TGFBIp trafficking PMID: 25853243
    30. TGFBI plays an important role in the progression of colorectal cancers PMID: 25755764
    31. Betaig-h3 protein expressed distinctly in gastric cancer peritoneal metastasis. PMID: 25864756
    32. The results support a novel inflammatory pathway for diabetic retinopathy. This pathway is initiated by TGFbeta released from macrophages, which promotes synthesis and release of BIGH3 protein by REC and REC apoptosis. PMID: 25378215
    33. Silencing of DDR1 inhibited tumor cell growth and motility, and induced TGFBI expression, both in vitro and in vivo. PMID: 25369402
    34. A de novo R124C mutation of the TGFBI gene may occur in one of dizygotic twins. Patients with an R124C mutation may have clinical features like corneal dystrophy of the Bowman layer. PMID: 25785536
    35. The patient in this reprot showed typical asymmetric ndulinear stromal opacity in both corneas, and an L527R mutation in the TGFBI gene with two SNP mutations. PMID: 24505203
    36. We present the characteristics and surgical treatment of corneas with a novel A620P mutation in TGFBI showing lattice corneal dystrophy type IIIA with hyaline component. PMID: 25321938
    37. Granular corneal dystrophy type II can co-exist with keratoconus and should be included in the differential diagnosis. PMID: 24582869
    38. TGFBI is a potential risk gene for human diabetes. PMID: 24728038
    39. a small difference in thermodynamic stability introduced by the P551Q mutation most likely causes structural changes of TGFBIp. PMID: 24302499
    40. The TGFBI gene, that is responsible for lattice corneal dystrophy type 1, is expressed above all by the corneal epithelial cells but also by the keratocytes PMID: 25055147
    41. Integrins alphavbeta5 and alphavbeta3 both control myofibroblast differentiation by activating latent TGF-beta1 PMID: 24639195
    42. These results reveal betaig-h3 enhances the metastasis potentials of human osteosarcoma cells via integrin alpha2beta1-mediated PI3K/AKT signal pathways. PMID: 24595049
    43. This study improves the knowledge of the genetic features of Mexican patients with corneal stromal dystrophies by identifying mutations in the TGFBI, CHST6, and GSN genes. PMID: 24801599
    44. Pin1 induction during liver fibrosis is involved in hepatic stellate cell activation, TGFbeta1 expression, and TGFbeta1-mediated fibrogenesis signalling. PMID: 24530597
    45. The H19-miR-675 axis acts as a suppressor of prostate cancer metastasis via modulation of TGFBI expression. PMID: 24988946
    46. Induced BIGH3 expression upon BM stress may contribute to the regulation of BM homeostasis PMID: 24152593
    47. A novel heterozygous missense mutation p.Leu565Pro was identified in exon 13 of the TGFBI gene. PMID: 24473223
    48. TGFBI dysregulation could occur in HBV-related HCC development. PMID: 24662304
    49. TGFBI is required for melanoma metastatic outgrowth; knockdown does not interfere with melanoma cells' dissemination to the lung PMID: 24499734
    50. Circulating levels of TGFBIp were significantly elevated in septic patients compared with healthy controls, and were strongly correlated with disease severity. TGFBIp enhanced vascular proinflammatory responses through interacting with integrin alphavbeta5. PMID: 24506343

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  • 相關疾病:
    Corneal dystrophy, epithelial basement membrane (EBMD); Corneal dystrophy, Groenouw type 1 (CDGG1); Corneal dystrophy, lattice type 1 (CDL1); Corneal dystrophy, Thiel-Behnke type (CDTB); Corneal dystrophy, Reis-Bucklers type (CDRB); Corneal dystrophy, lattice type 3A (CDL3A); Corneal dystrophy, Avellino type (CDA)
  • 亞細胞定位:
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 組織特異性:
    Highly expressed in the corneal epithelium. Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 數據庫鏈接:

    HGNC: 11771

    OMIM: 121820

    KEGG: hsa:7045

    STRING: 9606.ENSP00000416330

    UniGene: Hs.369397



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