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  4. Human T-complex protein 1 subunit zeta(CCT6A) ELISA kit

Human T-complex protein 1 subunit zeta(CCT6A) ELISA kit

  • 中文名稱：
    人T復(fù)合物蛋白1亞基ζ(CCT6A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL004865HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人T復(fù)合物蛋白1亞基ζ(CCT6A)酶聯(lián)免疫試劑盒（CSB-EL004865HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的CCT6A含量。CCT6A是一種分子伴侶蛋白，屬于CCT復(fù)合物成員，參與蛋白質(zhì)折疊。研究發(fā)現(xiàn)，CCT6A在多種腫瘤中促進腫瘤生長和轉(zhuǎn)移，其研究機制涉及調(diào)控細胞周期、增殖、侵襲和免疫抑制等過程。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，科研人員可將其用于探究CCT6A在腫瘤發(fā)生、神經(jīng)退行性疾病或代謝紊亂中的分子機制，也可用于評估細胞應(yīng)激條件下伴侶蛋白系統(tǒng)的功能變化，或篩選影響蛋白質(zhì)折疊通路的小分子化合物，為疾病基礎(chǔ)研究及藥物開發(fā)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    CCT6A ELISA Kit; CCT6 ELISA Kit; CCTZ ELISA Kit; T-complex protein 1 subunit zeta ELISA Kit; TCP-1-zeta ELISA Kit; Acute morphine dependence-related protein 2 ELISA Kit; CCT-zeta-1 ELISA Kit; HTR3 ELISA Kit; Tcp20 ELISA Kit
  • 縮寫：
    CCT6A
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CCT6A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %84-95
    1:2Average %94
    Range %88-98
    1:4Average %92
    Range %83-97
    1:8Average %98
    Range %94-102
  • 回收率：
    The recovery of human CCT6A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9894-103
    EDTA plasma (n=4)8580-90
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    16002.561 2.445 2.503 2.371
    8002.115 2.017 2.066 1.934
    4001.382 1.424 1.403 1.271
    2000.741 0.736 0.739 0.607
    1000.422 0.414 0.418 0.286
    500.264 0.255 0.260 0.128
    250.191 0.197 0.194 0.062
    00.133 0.131 0.132
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. The TRiC complex plays a role in the folding of actin and tubulin (Probable).
  • 基因功能參考文獻：
    1. High expression of CCT6A is associated with Non-Small Cell Lung Cancer. PMID: 28104536
    2. selective inhibition of SMAD3 or CCT6A efficiently suppresses TGF-beta-mediated metastasis. Findings provide a mechanism that directs TGF-beta signaling toward its prometastatic arm and may contribute to the development of therapeutic strategies targeting TGF-beta for non-small-cell lung carcinoma. PMID: 28375158
    3. These data suggest that CCT6A is a novel autoantigen recognized by Vdelta2 gammadelta T cells, which deepens our understanding of mechanisms in autoimmune diseases. PMID: 27489109
    4. identified genes, which include Tcp20, may play important role in conferring radioresistance to oral squamous cell carcinoma and could be useful in identifying cases with more radioresistance PMID: 16211299
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    TCP-1 chaperonin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1620

    OMIM: 104613

    KEGG: hsa:908

    STRING: 9606.ENSP00000275603

    UniGene: Hs.82916



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