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  4. Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1(SCUBE1)ELISA Kit

  • 中文名稱：
    人信號肽,CUB和EGF樣結構域蛋白1(SCUBE1)酶聯免疫試劑盒
  • 貨號：
    CSB-E15005h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人信號肽,CUB和EGF樣結構域蛋白1(SCUBE1)酶聯免疫試劑盒（CSB-E15005h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SCUBE1含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于基礎科研中探索SCUBE1在疾病模型中的表達動態、信號通路關聯性分析，或作為分子標志物在細胞實驗、動物模型及臨床前樣本中的定量評估，為研究血管生成、組織修復及病理機制提供可靠工具。滿足分子生物學及轉化醫學研究的精準檢測需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    SCUB1_HUMAN ELISA Kit; SCUBE 1 ELISA Kit; SCUBE1 ELISA Kit; Signal peptide, CUB and EGF-like domain-containing protein 1 ELISA Kit; Signal peptide, CUB domain, EGF like 1 ELISA Kit
  • 縮寫：
    SCUBE1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SCUBE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %89
    Range %82-94
    1:10Average %96
    Range %91-105
    1:20Average %99
    Range %95-108
    1:40Average %94
    Range %86-102
  • 回收率：
    The recovery of human SCUBE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10699-110
    EDTA plasma (n=4)9284-96
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.670 2.592 2.631 2.502
    202.271 2.173 2.222 2.093
    101.631 1.615 1.623 1.494
    50.940 0.925 0.933 0.804
    2.50.512 0.502 0.507 0.378
    1.250.357 0.347 0.352 0.223
    0.6250.226 0.219 0.223 0.094
    00.130 0.127 0.129 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.
  • 基因功能參考文獻：
    1. The results of this pioneering study indicate that SCUBE protein family appears to have a probable role in the pathogenesis and angiogenesis development in psoriasis and SCUBE 1 and 3 may be novel markers of angiogenesis in psoriasis. PMID: 28238185
    2. enhanced SCUBE1 concentrations are correlated with increasing severity and poor outcomes of aneurysmal subarachnoid hemorrhage patients, indicating SCUBE1 might have the potential to identify aneurysmal subarachnoid hemorrhage patients at risk of poor prognosis PMID: 27265525
    3. Increased serum SCUBE1 concentrations have close relation to increasing severity and poor prognosis of intracerebral hemorrhage PMID: 27496080
    4. SCUBE1 might be a novel prognostic biomarker after traumatic brain injury PMID: 26940951
    5. describe novel mechanisms in targeting S1 to the plasma membrane and demonstrate that N-glycans are required for S1 functions during primitive haematopoiesis in zebrafish. PMID: 26699903
    6. SCUBE1 may indicate hypercoagulability in patients with BC and thus help identify patients at greater risk of thrombosisSCUBE1 may indicate hypercoagulability in patients with breast cancer and thus help identify patients at greater risk of thrombosis PMID: 26438214
    7. SCUBE1 levels are a new biomarker that can be used in the differential diagnosis and monitoring of patients hospitalized with suspected CCHF. PMID: 23632301
    8. Primary prostatic cancer-associated fibroblasts (CAFs) were isolated and the expression of CAF markers, Dlk1 and SCUBE1 transcripts were characterised. PMID: 23136397
    9. SCUBE 1 levels in patients with gastric cancer were found higher compared to healthy subjects. PMID: 23220851
    10. SCUBE1 level, a potential acute ischemia marker, is elevated in hemodialysis patients with no clinical ischemic event. PMID: 22874483

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  • 亞細胞定位：
    Secreted. Cell membrane; Peripheral membrane protein.
  • 組織特異性：
    Detected in endothelial cells. Highly expressed in platelets. Stored in platelet alpha granules, and transferred to the cell surface upon activation and aggregation. A smaller form, probably produced by limited proteolysis, after being released from the s
  • 數據庫鏈接：

    HGNC: 13441

    OMIM: 611746

    KEGG: hsa:80274

    STRING: 9606.ENSP00000354080

    UniGene: Hs.133995



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