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Human Septin-2(SEPT2) ELISA kit

  • 中文名稱:
    人Septin-2(SEPT2)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL021025HU
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人Septin-2(SEPT2)酶聯(lián)免疫試劑盒(CSB-EL021025HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SEPT2含量。SEPT2 是一個值得關(guān)注的靶點。它屬于 Septin 家族,在細(xì)胞分裂、細(xì)胞骨架形成等多種細(xì)胞過程中發(fā)揮作用。研究常圍繞其在腫瘤發(fā)生發(fā)展中的機制展開,探討其與腫瘤細(xì)胞增殖、遷移、侵襲等行為的關(guān)系,有望為腫瘤治療提供新方向。試劑盒檢測范圍為25 pg/mL-1600 pg/mL,適用于基礎(chǔ)科研領(lǐng)域中對SEPT2蛋白表達的定量分析,例如探究其在細(xì)胞周期調(diào)控、腫瘤微環(huán)境變化或神經(jīng)細(xì)胞功能研究中的作用機制,也可用于評估不同實驗條件下(如藥物處理、基因編輯)SEPT2的表達動態(tài)變化,為相關(guān)疾病的分子機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    SEPTIN2 ELISA Kit; DIFF6 ELISA Kit; KIAA0158 ELISA Kit; NEDD5 ELISA Kit; SEPT2 ELISA Kit; Septin-2 ELISA Kit; Neural precursor cell expressed developmentally down-regulated protein 5 ELISA Kit; NEDD-5 ELISA Kit
  • 縮寫:
    SEPT2
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    25 pg/mL-1600 pg/mL
  • 靈敏度:
    6.25 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human SEPT2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 81-89  
    1:2 Average % 98  
    Range % 93-101  
    1:4 Average % 83  
    Range % 80-86  
    1:8 Average % 99  
    Range % 94-102  
  • 回收率:
    The recovery of human SEPT2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 108 104-112  
    EDTA plasma (n=4) 95 91-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1600 2.207 2.247 2.227 2.080  
    800 1.786 1.798 1.792 1.645  
    400 1.365 1.308 1.337 1.190  
    200 0.814 0.842 0.828 0.681  
    100 0.592 0.568 0.580 0.433  
    50 0.355 0.367 0.361 0.214  
    25 0.248 0.238 0.243 0.096  
    0 0.146 0.148 0.147    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能:
    Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
  • 基因功能參考文獻:
    1. Results demonstrate that in stored platelets, septine-2 and septin-6 mRNAs have miR- 223 target sites, septin-2 and septin-6 are in complex with Ago-2. The results demonstrate that like in nucleated cells, enucleated platelets also have microRNA-based mechanisms for the regulation of their septins. PMID: 29943706
    2. Study discloses both SEPT2 and SEPT7 are essential for breast cancer cell migration and invasion by controlling MEK/ERK MAPKs activation. PMID: 27557506
    3. Depletion of septin 2 reduces Drp1 recruitment to mitochondria and results in hyperfused mitochondria and delayed FCCP-induced fission. PMID: 27215606
    4. septins protect ErbB2 from ubiquitylation, endocytosis and lysosomal degradation. Septin oligomerization regulates persistent expression of ErbB2/HER2 in gastric cancer cells. PMID: 27048593
    5. The results showed that SEPT2 has an oncogenic function through accelerated cell proliferation and invasion in biliary tract cancers, and is negatively regulated by miR-140-5p. PMID: 27155525
    6. From the nucleotide-free structure some interesting conclusions about the nucleotide binding properties of Cdc11 can be drawn; especially when aligning the structure with the structure of GDP bound Sept2 PMID: 26780475
    7. These results suggest that SEPTIN2-mediated cytoskeletal rearrangement and STATHMIN-mediated differentiation may contribute to changes in cell morphology and differentiation of H/RS cells with CD99 upregulation in Hodgkin lymphoma. PMID: 26000982
    8. Data indicate that forchlorfenuron (FCF) exhibits differential binding preference for septins SEPT2 and SEPT3. PMID: 24787956
    9. Authors report here that the septins SEPT2, -9, -11, and probably -7 form fibrillar structures around the chlamydial inclusion. PMID: 25293760
    10. Suggest PPAR-gamma activation down-regulates hepatocellular carcinoma cell SEPT2 levels to prevent tumor growth. PMID: 25592041
    11. SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9. PMID: 23572511
    12. The results shown in study support the hypothesis that single Septin 2, when present in excess or with unbalanced stoichiometries, may be unstable and assemble into amyloid-like structures. PMID: 21967827
    13. Data illustrated roles of SEPT9 that might contribute to hetero-trimeric septin complex formation. SEPT9 can substitute for septins of the SEPT2 group and partially for SEPT7. PMID: 21767235
    14. The SEPT2 provides the directional guidance cues necessary for polarizing the epithelial microtubule network. PMID: 21788367
    15. septin-2 plays a fundamental role in regulating barrier function by altering cortical actin expression. PMID: 20870893
    16. the role of SEPT2 and SEPT11 in the InlB-Met interactions PMID: 21504731
    17. Data show that Septins of the SEPT6 group preferentially interacted with septins of the SEPT2 group, SEPT3 group and SEPT7 group. PMID: 21082023
    18. Nedd5 is involved in the process of cytokinesis in human brain tumours. PMID: 12125979
    19. septins may form a mitotic scaffold for CENP-E and other effectors to coordinate cytokinesis with chromosome congression and segregation PMID: 15774761
    20. Nedd5 C-terminal is a novel autoantigen in systemic lupus erythematosus (SLE) patients and may be a valuable tool for diagnosing neuropsychiatric SLE. PMID: 15987492
    21. protein encoded by SEPT2 is highly homologous to septins 1, 4 and 5 and is involved in the coordination of several key steps of mitosis PMID: 16682951
    22. septin 2, 6, and 7 complexes make up polymerized filaments PMID: 16914550
    23. crystal structures of the human SEPT2 G domain and the heterotrimeric human SEPT2-SEPT6-SEPT7 complex PMID: 17637674
    24. Study shows that in epithelial cells septin 2 fibers colocalize with a subset of microtubule tracks composed of polyglutamylated tubulin. PMID: 18209106
    25. A novel MLL-SEPT2 fusion variant in therapy-related myelodysplastic syndrome is reported. PMID: 18656699
    26. confirmed that the phosphorylation of SEPT2 on Ser218 by CK2 was crucial to the proliferation of HCC. These results suggest that SEPT2 might be a promising target for liver cancer therapy PMID: 19165576
    27. SEPT2 is essential for the InlB-mediated entry of Listeria, but SEPT11 is not, which distinguishes the roles of different mammalian septins PMID: 19234302

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  • 亞細(xì)胞定位:
    Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Midbody. Cytoplasm, cell cortex. Cell projection, cilium membrane. Cell projection, cilium, flagellum.
  • 蛋白家族:
    TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family
  • 組織特異性:
    Widely expressed. Up-regulated in liver cancer.
  • 數(shù)據(jù)庫鏈接:

    HGNC: 7729

    OMIM: 601506

    KEGG: hsa:4735

    STRING: 9606.ENSP00000353157

    UniGene: Hs.721234



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