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Human Semaphorin-3G(SEMA3G) ELISA kit

  • 中文名稱:
    人臂板蛋白-3G(SEMA3G)酶聯免疫試劑盒
  • 貨號:
    CSB-EL020986HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人臂板蛋白-3G(SEMA3G)酶聯免疫試劑盒(CSB-EL020986HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SEMA3G含量。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL,適用于基礎科研中對SEMA3G表達水平的精準分析;為探索SEMA3G在神經系統發育、腫瘤血管生成或免疫調控中的分子機制提供可靠工具,也可用于評估疾病模型或藥物干預下SEMA3G的濃度變化;支持科研人員在體外實驗中對SEMA3G進行高通量定量檢測,適用于細胞生物學、病理機制研究及生物標志物篩選等非臨床研究場景本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    FLJ00014 ELISA Kit; MGC119473 ELISA Kit; Sem2 ELISA Kit; SEM3G_HUMAN ELISA Kit; Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3G ELISA Kit; Sema3g ELISA Kit; Semaphorin 3G ELISA Kit; Semaphorin sem2 ELISA Kit; Semaphorin-3G ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.625 ng/mL-40 ng/mL
  • 靈敏度:
    0.156 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human SEMA3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 97
    Range % 91-102
    1:2 Average % 101
    Range % 96-105
    1:4 Average % 94
    Range % 87-98
    1:8 Average % 99
    Range % 91-103
  • 回收率:
    The recovery of human SEMA3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 82-91
    EDTA plasma (n=4) 97 90-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.005 1.941 1.973 1.824
    20 1.613 1.576 1.595 1.446
    10 1.144 1.109 1.127 0.978
    5 0.746 0.733 0.740 0.591
    2.5 0.544 0.524 0.534 0.385
    1.25 0.383 0.372 0.378 0.229
    0.625 0.275 0.269 0.272 0.123
    0 0.152 0.146 0.149  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Has chemorepulsive activities for sympathetic axons. Ligand of NRP2.
  • 基因功能參考文獻:
    1. Results indicate that Sema3G protein is secreted by podocytes and protects them from inflammatory kidney diseases and diabetic nephropathy. PMID: 27180624
    2. Results identify Sema3g as one of the downstream effectors of PPAR-gamma, which is centrally involved in regulating endothelial cell migration. PMID: 25335934
    3. overexpressed SEMA3G inhibited the migratory and invasive behavior of U251MG cancer cells PMID: 22562223
    4. Identify Sema3G as a primarily endothelial cell-expressed class 3 semaphorin that controls endothelial and smooth muscle cell functions in autocrine and paracrine manners. PMID: 20947821
    5. In gliomas, SEMA3B, SEMA3G and NRP2 expressions were related to prolonged survival PMID: 18781179
    6. Sema3G inhibits tumor progression from MDA-MB-435 but not from MDA-MB-231 cancer cells. It inhibits tumor angiogenesis in MDA-MB-231 cells. PMID: 18818766

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Semaphorin family
  • 數據庫鏈接:

    HGNC: 30400

    KEGG: hsa:56920

    STRING: 9606.ENSP00000231721

    UniGene: Hs.59729



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