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  4. Human Secreted frizzled-related protein 2(SFRP2) ELISA kit

Human Secreted frizzled-related protein 2(SFRP2) ELISA kit

  • 中文名稱：
    人分泌型卷曲相關蛋白2(SFRP2)酶聯免疫試劑盒
  • 貨號：
    CSB-EL021139HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人分泌型卷曲相關蛋白2(SFRP2)酶聯免疫試劑盒（CSB-EL021139HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、細胞裂解物樣本中的SFRP2含量。SFRP2是一種分泌型糖蛋白，屬于分泌型卷曲相關蛋白家族。它參與Wnt信號通路的調控，在胚胎發育等過程中發揮重要作用。研究發現其異常表達與腫瘤等多種疾病的發生發展相關，深入研究其作用機制有望為疾病治療提供新靶點與策略。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，在科研領域可應用于腫瘤微環境分析、干細胞分化研究、骨骼發育調控等方向，尤其適合探究SFRP2在結直腸癌、骨質疏松等疾病模型中的表達變化及其功能機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    AI851596 ELISA Kit; Frizzled related protein 2 ELISA Kit; FRP 2 ELISA Kit; FRP-2 ELISA Kit; FRP2 ELISA Kit; MGC128911 ELISA Kit; MGC153618 ELISA Kit; MGC53344 ELISA Kit; SARP 1 ELISA Kit; SARP-1 ELISA Kit; SARP1 ELISA Kit; SDF 5 ELISA Kit; SDF5 ELISA Kit; Secreted apoptosis related protein 1 ELISA Kit; Secreted apoptosis-related protein 1 ELISA Kit; Secreted frizzled related protein 2 ELISA Kit; Secreted frizzled-related protein 2 ELISA Kit; sFRP 2 ELISA Kit; sFRP-2 ELISA Kit; SFRP2 ELISA Kit; Sfrp2 protein ELISA Kit; SFRP2_HUMAN ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human SFRP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-103
    1:2Average %91
    Range %85-98
    1:4Average %92
    Range %88-96
    1:8Average %99
    Range %96-104
  • 回收率：
    The recovery of human SFRP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-94
    EDTA plasma (n=4)9690-102
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.206 2.119 2.163 2.026
    51.535 1.650 1.593 1.456
    2.51.125 1.103 1.114 0.977
    1.250.766 0.745 0.756 0.619
    0.6250.521 0.554 0.538 0.401
    0.3120.350 0.327 0.339 0.202
    0.1560.253 0.268 0.261 0.124
    00.138 0.136 0.137
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:


Could you please find out which antigen (expression system) is included in the standard? I would like to spike the samples with this antigen.

A:
Thanks for your inquiry! CSB-EL021139HU: Standard of this kit is 293 cell-derived recombinant protein.
We make the RECOVERY with this kit and will send you QC for your checking.

靶點詳情

  • 功能：
    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
  • 基因功能參考文獻：
    1. SFRP2 was downregulated in Choriocarcinoma and low expressed SFRP2 could promote migration, invasion, the epithelial-mesenchymal transition phenotype and stemness of Choriocarcinoma cells via Wnt/beta-catenin signaling PMID: 30396168
    2. Results show that SFRP2 and SFRP4 typically associate with a poor prognosis of cancer patients concomitant with the expression of genes associated with epithelial-to-mesenchymal transition. SFRP2 and 4 are likely derived from the tumor stroma, and thus tend to increase in tumors as compared to normal tissues. PMID: 28218291
    3. The content of the methylated SFRP2 gene in endometrial tissue of patients with hyperplastic processes higher than 20-25% allows relate these women to the risk group of EC development and dictates the need of intensive observation of such patients. PMID: 29949535
    4. Findings demonstrated that SFRP2 was downregulated in pituitary corticotroph adenomas. SFRP2 appears to act as a tumor suppressor in Cushing's disease by regulating the activity of the Wnt signaling pathway. PMID: 29620167
    5. SFRP2 was down-regulated in the accelerated and blast phase of CML, whereas, the levels of WNT1, WNT3 and WNT5A were up-regulated in the accelerated and blast phase of CML. Overexpression SFRP2 inhibited proliferation, promoted apoptosis and activated the WNT pathway. PMID: 29704505
    6. Methylation of SFRP2 gene may promote the invasiveness of non-small cell lung cancer cells through ZEB1 and MMP9 signaling. PMID: 29320940
    7. the differences in SFRP2 promotor methylation observed between colorectal cancer (CRC)patients and healthy individuals by both assays were statistically significant (p<0.001). Our findings, although limited by the small sample size, do not support the use of the MSRH assay for CRC screening in stool. PMID: 28777432
    8. augments Wnt3a-mediated beta-catenin signalling in dermal papilla cells, especially in beard dermal papilla cells PMID: 26914690
    9. SFRP2 methylation is associated with colorectal cancer development. PMID: 26258809
    10. TET1 potently inhibited canonical Wnt/beta-catenin signaling by demethylating and upregulating two upstream antagonists of this pathway, SFRP2 and DKK1, which was associated with inhibition of EMT and cancer cell metastasis. PMID: 28851501
    11. SFRP2 could bind to locally present Wnt ligands and alter the balance of intracellular Wnt signaling to antagonize the canonical Wnt pathway in stem cells from the apical papilla. PMID: 28794794
    12. SFRP2 induction is remarkable in tumor stroma, with transcription mainly modulated by the nuclear factor-kappaB (NF-kappaB) complex, a property shared by several effectors of the DNA damage secretory program. PMID: 26751775
    13. down-regulated in ICU-acquired weakness and mice with inflammation-induced muscle atrophy; possibly establishes a positive feedback loop enhancing TGF-beta1-mediated atrophic effects in inflammation-induced atrophy PMID: 27661566
    14. epigenetic silencing of Wnt antagonists was associated with gastric carcinogenesis, and concurrent hypermethylation of SFRP2 and DKK2 could be a potential marker for a prognosis of poor overall survival. PMID: 28152305
    15. SFRP2 enhances the adipogenic and neurogenic differentiation potentials of stem cells from apical papilla by up-regulating SOX2 and OCT4. PMID: 28244619
    16. SFRP2 is not only an agonist of Wnt pathway, but also a cancer promoting protein for lung cancer. PMID: 26323494
    17. DKK1 (dickkopf-1) and SFRP2 (secreted frizzled-related protein 2) were the targets of miR-522 in hepatocellular carcinoma PMID: 26960688
    18. the demethylation of SFRP2 gene appeared to inhibit nuclear retention of a key Wnt signaling factor, b-catenin, in osteosarcoma (OS) cell lines. Together, these data suggest that SFRP2 may function as an OS invasion suppressor by interfering with Wnt signaling, and the methylation of SFRP2 gene may promote pathogenesis of OS PMID: 26628297
    19. SFRP2 hypermethylation is associated with colorectal cancer. PMID: 27221916
    20. Silencing KDM2A, a histone demethylase and BCL6 co-repressor, de-repressed SFRP2 transcription by increasing histone H3K4 and H3K36 methylation at the SFRP2 promoter. PMID: 27074224
    21. SFRP2 protein plays a role in the ultraviolet rays induced hyperpigmentary disorders. PMID: 26763443
    22. age-related increases in sFRP2 augment both angiogenesis and metastasis of melanoma cells PMID: 27042933
    23. Patients with Cytogenetically Normal Primary Acute Myeloid Leukemia and high sFRP2 expression had higher incidence of complete remission rate and better overall survival. PMID: 26517501
    24. A combination of GATA5 and SFRP2 methylation could be promising as a marker for the detection and diagnosis of colorectal cancers and adenomas. PMID: 25759530
    25. The distribution of Sfrp2 (and Sfrp1) in the eye is consistent with the idea that they modulate visual pathfinding and axon guidance. PMID: 25788689
    26. These findings suggest that decreased SFRP2 expression is associated with intermediate/poor karyotypes in acute myeloid leukemia PMID: 25197341
    27. The role of SFRP2, as a functional tumor suppressor in the development of oral squamous cell carcinoma, is mediated through inhibition of the Wnt signaling pathway. PMID: 25189527
    28. Data suggest that overexpression of secreted frizzled-related protein 2 (sFRP2) gene in mesenchymal stem cells (hMSCs) might improve the therapeutic effectiveness of hMSC transplantation. PMID: 25245632
    29. Aberrant methylation of APC gene was statistically significant associated with age over 50, DDK3 with male, SFRP4, WIF1, and WNT5a with increasing tumor stage SFRP4 and WIF1 with tumor differentiation and SFRP2 and SFRP5 with histological type PMID: 25107489
    30. In hepatitis C virus infected liver tissues hypermethylation at promoter regions of key cancer related genes SFRP2 and DKK1, appears early at chronic hepatitis and liver cirrhosis stages, long before the appearance of hepatocellular carcinoma. PMID: 24947038
    31. High SFRP2 gene methylation is associated with ovarian cancer infected with high risk human papillomavirus. PMID: 24761891
    32. The risk size of SFRP2 hypermethylation from normal control to adenoma or polyp as well as from adenoma or polyp to colorectal cancer increased gradually PMID: 25053594
    33. results show that sFRP-2 is a target gene of hypermethylation in esophageal Basaloid squamous cell carcinoma PMID: 24464051
    34. SFRP2 appears to interact with Slug to affect the apoptosis of hypertrophic scar fibroblasts PMID: 23226515
    35. show that SFRP2 hypermethylation is a common event in prostate cancer. SFRP2 methylation in combination with other epigenetic markers may be a useful biomarker of prostate cancer PMID: 22915211
    36. Promoter hypermethylation of tumor suppressor SFRP2 is associated with prostate carcinoma. PMID: 22136354
    37. Recombinant sFRP2 enhanced Wnt3a-dependent phosphorylation of LRP6 as well as both cytosolic beta-catenin levels and its nuclear translocation. sFRP2 enhanced the Wnt3a-mediated transcription of several genes including DKK1 and NKD1. PMID: 20723538
    38. Data suggest that silencing of secreted frizzled-related protein 2 expression through promoter hypermethylation may be a factor in ESCC carcinogenesis through loss of its tumor-suppressive activity. PMID: 22363119
    39. there is a loss of SFRP-2 expression from benign to malignant prostate glands and differential SFRP-2 expression among two possible subgroups of Gleason grade 5 tumours PMID: 22175903
    40. Hypermethylation of SFRP2 gene is associated with advanced gastric cancer. PMID: 21409489
    41. SFRP2 may play an important role in the development of earlobe keloid, especially at the keloid edge. PMID: 21174795
    42. A study evaluating the pattern of SFRP2 methylation throughout the promoter during progressive tumorigenesis. PMID: 21709714
    43. Among the extracellular regulators that suppress the Wnt pathway, secreted frizzled-related protein 2 (SFRP2), was up-regulated 4.3-fold in healthy smokers and 4.9-fold in COPD smokers. PMID: 21490961
    44. serum SFRP2 methylation status represents a promising, non-invasive marker for colorectal carcinoma detection and staging PMID: 21463549
    45. Combined effects of epigenetic alterations in SFRP2 and point mutations in K-ras protein play a role in development of mucinous type anal adenocarcinoma. PMID: 20686305
    46. Promoter hypermethylation of SFRP2 is associated with Acute myeloid leukemia. PMID: 20795789
    47. SFRP2 promoter methylation is aberrant in mesothelioma. PMID: 20596629
    48. induces transient rise of intracellular Ca2+ via emptying of intracellular calcium stores in neutrophils PMID: 20602801
    49. These results support sFRP2's role as an enhancer of Wnt3A/beta-catenin signaling, a result with biological impact for both normal development and diverse pathologies such as tumorigenesis. PMID: 20723538
    50. This is the first report documenting that sFRP2 activates the canonical Wnt pathway and promotes cell growth by evoking diverse signaling cascades in renal cancer cells. PMID: 20501806

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Secreted frizzled-related protein (sFRP) family
  • 組織特異性：
    Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.
  • 數據庫鏈接：

    HGNC: 10777

    OMIM: 604157

    KEGG: hsa:6423

    STRING: 9606.ENSP00000274063

    UniGene: Hs.481022



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