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  4. Human Reticulon-4 receptor-like 2(RTN4RL2) ELISA kit

Human Reticulon-4 receptor-like 2(RTN4RL2) ELISA kit

  • 中文名稱：
    人網狀蛋白4受體樣蛋白2(RTN4RL2)酶聯免疫試劑盒
  • 貨號：
    CSB-EL020576HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人網狀蛋白4受體樣蛋白2(RTN4RL2)酶聯免疫試劑盒（CSB-EL020576HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的RTN4RL2含量。RTN4RL2，一種人網狀蛋白4受體樣蛋白，參與細胞表面受體信號通路、胼胝體發育和負調節。其研究機制涉及神經元軸突導向、髓鞘形成及損傷后神經元保護。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產品可廣泛應用于神經生物學、細胞信號轉導等基礎研究領域，支持體外培養細胞模型、動物實驗組織樣本或體液樣本中RTN4RL2蛋白水平的精準定量分析，為探索其在神經再生、疾病機制中的功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    RTN4RL2 ELISA Kit; NGRH1 ELISA Kit; NGRL3 ELISA Kit; Reticulon-4 receptor-like 2 ELISA Kit; Nogo receptor-like 3 ELISA Kit; Nogo-66 receptor homolog 1 ELISA Kit; Nogo-66 receptor-related protein 2 ELISA Kit; NgR2 ELISA Kit
  • 縮寫：
    RTN4RL2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human RTN4RL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %90-106
    1:2Average %95
    Range %85-104
    1:4Average %95
    Range %86-100
    1:8Average %96
    Range %88-109
  • 回收率：
    The recovery of human RTN4RL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9684-107
    EDTA plasma (n=4)9290-95
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.081 2.017 2.049 1.947
    51.777 1.758 1.768 1.666
    2.51.337 1.326 1.332 1.230
    1.250.826 0.849 0.838 0.736
    0.6250.517 0.531 0.524 0.422
    0.3120.338 0.345 0.342 0.240
    0.1560.273 0.289 0.281 0.179
    00.103 0.101 0.102
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Cell surface receptor that plays a functionally redundant role in the inhibition of neurite outgrowth mediated by MAG. Plays a functionally redundant role in postnatal brain development. Contributes to normal axon migration across the brain midline and normal formation of the corpus callosum. Does not seem to play a significant role in regulating axon regeneration in the adult central nervous system. Protects motoneurons against apoptosis; protection against apoptosis is probably mediated by MAG. Like other family members, plays a role in restricting the number dendritic spines and the number of synapses that are formed during brain development. Signaling mediates activation of Rho and downstream reorganization of the actin cytoskeleton.
  • 基因功能參考文獻：
    1. Authors highlight the structural and biochemical aspects of the interaction of Nogo receptors (R1 and R2) with myelin inhibitors such as MAG, Nogo A and OMgp.[Review] PMID: 24956133
    2. These results indicate that a lack of NgR1/2 expression promotes the adhesion of DCs to myelin. PMID: 21906273
    3. predominantly expressed in the neurons of the embryonic and adult nervous systems PMID: 14664809
    4. The release of N-terminal fragment-NgRH1 was blocked by a hydroxamate-based zinc metalloproteinase inhibitor and tissue inhibitor of metalloproteinases-2 and -3 PMID: 15629437
    5. Nogo-66 receptor homolog 1 signaling regulates activity-dependent synaptic strength and neuronal plasticity. PMID: 18337405

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  • 亞細胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor. Membrane raft. Cell projection, dendrite. Perikaryon. Cell projection, axon.
  • 蛋白家族：
    Nogo receptor family
  • 組織特異性：
    Highly expressed in brain and liver. Expressed at lower levels in kidney, mammary gland, placenta, skeletal muscle, spleen and thyroid.
  • 數據庫鏈接：

    HGNC: 23053

    OMIM: 610462

    KEGG: hsa:349667

    STRING: 9606.ENSP00000335397

    UniGene: Hs.502618



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