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  4. Human RING finger protein 207(RNF207) ELISA kit

Human RING finger protein 207(RNF207) ELISA kit

  • 中文名稱：
    人環指蛋白207(RNF207)酶聯免疫試劑盒
  • 貨號：
    CSB-EL019870HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人環指蛋白207(RNF207)酶聯免疫試劑盒（CSB-EL019870HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的RNF207含量。RNF207是一種E3泛素連接酶，參與蛋白質泛素化過程，與心肌病和心臟能量代謝相關。研究發現，RNF207在心肌細胞中表達，可能通過影響HERG蛋白的轉運和定位來調節動作電位持續時間，從而影響心臟功能。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，本試劑盒檢測靈敏度為15.6 pg/mL，線性檢測范圍覆蓋15.6-1000 pg/mL，可精準測定低豐度樣本中RNF207的表達量，適用于探索其在心肌細胞代謝、組織損傷修復或病理狀態下的分子機制研究。提供標準品、檢測抗體及全套優化緩沖液，支持科研人員對心血管疾病模型、基因編輯細胞系或藥物干預實驗中的RNF207動態變化進行定量分析，為功能研究與分子互作機制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    RNF207 ELISA Kit; C1orf188 ELISA Kit; RING finger protein 207 ELISA Kit
  • 縮寫：
    RNF207
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human RNF207 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %86
    Range %81-90
    1:4Average %93
    Range %89-98
    1:8Average %90
    Range %85-94
  • 回收率：
    The recovery of human RNF207 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-100
    EDTA plasma (n=4)10195-108
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.719 2.519 2.619 2.500
    5002.172 2.072 2.122 2.003
    2501.504 1.607 1.556 1.437
    1250.996 1.061 1.029 0.910
    62.50.653 0.623 0.638 0.519
    31.20.362 0.380 0.371 0.252
    15.60.252 0.261 0.257 0.138
    00.123 0.115 0.119
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Plays a role in cardiac repolarization possibly by stabilizing membrane expression of the potassium channel KCNH2/HERG, or by assisting its synthesis, folding or export from the endoplasmic reticulum, in a heat shock protein-dependent manner.
  • 基因功能參考文獻：
    1. Data indicate that plasma E3 Ligase Rnf207 (ring finger protein 207) level in acute myocardial infarction patients increased significantly compared with that in healthy people. PMID: 25599194
    2. RNF207 is an important regulator of action potential duration, likely via effects on HERG trafficking and localization in a heat shock protein-dependent manner. PMID: 25281747
    3. Meta-analysis and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19305408
    4. Genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19305409
  • 亞細胞定位：
    Cytoplasm.
  • 數據庫鏈接：

    HGNC: 32947

    OMIM: 616923

    KEGG: hsa:388591

    STRING: 9606.ENSP00000367173

    UniGene: Hs.716549



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