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  4. Human Protein-glutamine gamma-glutamyltransferase 4(TGM4) ELISA kit

Human Protein-glutamine gamma-glutamyltransferase 4(TGM4) ELISA kit

  • 中文名稱：
    人蛋白-谷氨酰胺γ-谷氨酰轉移酶4(TGM4)酶聯免疫試劑盒
  • 貨號：
    CSB-EL023464HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人蛋白-谷氨酰胺γ-谷氨酰轉移酶4(TGM4)酶聯免疫試劑盒（CSB-EL023464HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的TGM4含量。試劑盒檢測范圍為50 pg/mL-3200 pg/mL，支持科研人員開展前列腺功能調控、生殖系統疾病機制探索或腫瘤微環境相關研究，為體外實驗提供穩定可靠的數據支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Dorsal prostate transglutaminase ELISA Kit; Dorsal protein 1 ELISA Kit; Dp1 ELISA Kit; Fibrinoligase ELISA Kit; hTGP ELISA Kit; Prostate specific transglutaminase ELISA Kit; Prostate transglutaminase ELISA Kit; Prostate-specific transglutaminase ELISA Kit; Protein glutamine gamma glutamyltransferase 4 ELISA Kit; Protein-glutamine gamma-glutamyltransferase 4 ELISA Kit; TG(P) ELISA Kit; TGase P ELISA Kit; TGase-4 ELISA Kit; TGase4 ELISA Kit; Tgm4 ELISA Kit; TGM4_HUMAN ELISA Kit; TGP ELISA Kit; Transglutaminase 4 (prostate) ELISA Kit; Transglutaminase 4 ELISA Kit; Transglutaminase P ELISA Kit; Transglutaminase, prostate ELISA Kit; Transglutaminase-4 ELISA Kit
  • 縮寫：
    TGM4
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    50 pg/mL-3200 pg/mL
  • 靈敏度：
    12.5 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TGM4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 86
    Range % 81-97
    1:2 Average % 90
    Range % 83-100
    1:4 Average % 95
    Range % 89-110
    1:8 Average % 95
    Range % 91-99
  • 回收率：
    The recovery of human TGM4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-104
    EDTA plasma (n=4) 93 88-103
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    3200 2.724 2.705 2.715 2.622
    1600 2.058 2.017 2.038 1.945
    800 1.382 1.369 1.376 1.283
    400 0.907 0.924 0.916 0.823
    200 0.581 0.584 0.583 0.490
    100 0.331 0.339 0.335 0.242
    50 0.222 0.220 0.221 0.128
    0 0.091 0.095 0.093 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract.
  • 基因功能參考文獻：
    1. TGM4 is a prostatic secretory molecule with critical role in male reproduction. PMID: 26084804
    2. indicated that transglutaminase 4 may serve as a potential predictor of biochemical recurrence of prostate cancer PMID: 23974364
    3. Evidence that the retinoic acid receptor gamma plays a major role in the regulation of the hTGP gene and that presence of the androgen receptor, but not its transcriptional transactivation activity, is critical for hTGP transcription. PMID: 22362749
    4. CDKN2A, GATA3, CREBBP, ITGA2, NBL1 and TGM4 were down-regulated in the prostate carcinoma glands compared to the corresponding normal glands PMID: 21743959
    5. The presence of TGase-4 has a biological impact on a prostate cancer cell's response to MDA-7. PMID: 21524313
    6. TGase-4 expression is intrinsically linked to the activation of RON in prostate cancer cells and that this autoactivation of RON contributes to the increased cell motility in TGase-4 expressing cells. PMID: 20596668
    7. The expression status of human transglutaminase 4 in benign prostate hyperplasia (BPH) and prostate cancer was analyzed. PMID: 20177144
    8. transglutaminase 4 has a relatively wide profile of expression in human cancer cell lines PMID: 17552366
    9. TGase-4 plays a pivotal role in the interaction between endothelial cells and prostate cancer cells, an action which is independent of the ROCK pathway. PMID: 18983858

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  • 蛋白家族：
    Transglutaminase superfamily, Transglutaminase family
  • 組織特異性：
    Prostate.
  • 數據庫鏈接：

    HGNC: 11780

    OMIM: 600585

    KEGG: hsa:7047

    STRING: 9606.ENSP00000296125

    UniGene: Hs.438265



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