在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Human Protein Wnt-5b(WNT5B) ELISA kit

Human Protein Wnt-5b(WNT5B) ELISA kit

  • 中文名稱：
    人Wnt-5b蛋白(WNT5B)酶聯免疫試劑盒
  • 貨號：
    CSB-EL026139HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人Wnt-5b蛋白(WNT5B)酶聯免疫試劑盒（CSB-EL026139HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的WNT5B含量。WNT5B是一個靶點。它是Wnt蛋白家族成員，在胚胎發育、細胞遷移與極性調控等多方面發揮關鍵作用。其研究機制涉及與受體結合激活非經典Wnt信號通路，異常表達與腫瘤、炎癥等疾病相關，在臨床研究中受重點關注。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于基礎科研領域對WNT5B蛋白表達水平的動態監測，例如癌癥機制研究、再生醫學模型構建或信號通路交互作用分析。科研人員可通過該產品在體外實驗中探索WNT5B在疾病模型中的功能，或評估藥物干預對相關信號通路的調控效應，為分子機制研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    MGC2648 ELISA Kit; Protein Wnt-5b ELISA Kit; Wingless type MMTV integration site family, member 5B ELISA Kit; WNT 5B ELISA Kit; WNT 5B protein ELISA Kit; Wnt family member 5B ELISA Kit; Wnt5b ELISA Kit; WNT5B protein ELISA Kit; WNT5B_HUMAN ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Stem Cells
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of Human WNT5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %87-96
    1:2Average %98
    Range %94-102
    1:4Average %94
    Range %89-98
    1:8Average %85
    Range %80-89
  • 回收率：
    The recovery of Human WNT5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9690-102
    EDTA plasma (n=4)8580-90
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.663 2.767 2.715 2.616
    52.326 2.225 2.276 2.177
    2.51.675 1.578 1.627 1.528
    1.251.085 1.022 1.054 0.955
    0.6250.507 0.523 0.515 0.416
    0.3120.264 0.276 0.270 0.171
    0.1560.178 0.187 0.183 0.084
    00.099 0.098 0.099
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 基因功能參考文獻：
    1. exaggerated WNT-5B expression upon cigarette smoke exposure in the bronchial epithelium of COPD patients leads to TGF-beta/Smad3-dependent expression of genes related to airway remodelling PMID: 27126693
    2. the data suggest that WNT5B induces tube formation by regulating the expression of Snail and Slug proteins through activation of canonical and non-canonical WNT signalling pathways. PMID: 27593938
    3. Noncanonical Wnt signaling via Wnt5a/5b/11 may have role in the pathogenesis of aortic valve calcification. PMID: 27932350
    4. WNT-5B induces IL-6 and CXCL8 secretion in pulmonary fibroblasts. PMID: 27036869
    5. overexpression of WNT5B significantly increased cell proliferation, migration and invasion capacities of the COLO 205 cells in vitro. WNT5B may play an important role in the tumorigenesis of colorectal cancer. PMID: 27121420
    6. results suggest that Wnt5b-associated exosomes promote cancer cell migration and proliferation in a paracrine manner PMID: 27762090
    7. these results suggest that TGF-beta1 stimulates HSC-4 cell invasion through the Slug/Wnt-5b/MMP-10 signalling axis. PMID: 26861993
    8. This study identified WNT5B and CTNNBL1 for peak bone mineral density and body composition in males from the Han Chinese ethnic group. PMID: 26733379
    9. The interaction between Wnt isoforms and their LRP6 cognate receptor depends on the jutting loop present in Wnt3a but absent in Wnt5b. PMID: 25425204
    10. Results demonstrate that the Wnt5B pathway may play an important role in atypical teratoid rhabdoid tumor biology. PMID: 25246426
    11. Report increased Wnt5b levels in adriamycin treated liver cancer stem cells within 4h of treatment. PMID: 24944491
    12. This study shows that the WNT5B germline variant rs2010851 was significantly identified as a stage-dependent prognostic marker for CC patients after 5-fluorouracil-based adjuvant therapy. PMID: 23817222
    13. WNT5B is elevated in both in the tumor and the patients' serum from triple negative breast cancer. PMID: 24564888
    14. Knocking down Wnt5b expression reduced phospho-PKCA levels and cell migration. PMID: 24841200
    15. Ursolic acid suppressed the expression of Wnt5alpha/beta and beta-catenin, inducing apoptosis in prostate cancer cells. PMID: 24399733
    16. These results indicate that Wnt5b is involved in the migration ability of OSCC cells through active Cdc42 and RhoA. PMID: 24220306
    17. WNT3 and WNT5B are critical factors, secreted from mesenchymal cancer cells, for instigating the epithelial cancer cell invasion. PMID: 24344732
    18. TGF-beta/Smad, integrin/integrin-linked kinase (ILK) and wnt/beta-catenin signaling pathways are involved in epithelial to mesenchymal transition in human renal fibrogenesis. PMID: 24040439
    19. Differentiation paralleled the activation of Wnt5/Calmodulin signalling by autocrine/paracrine intense secretion of Wnt5a and Wnt5b PMID: 21980498
    20. This is significantly related to fat cell differentiation, suggesting its possible involvement in the onset of diabetes mellitus type 2 and metabolic syndrome. PMID: 21766608
    21. WNT5b is upregulated by beta-estradiol in tumor cell lines PMID: 12165812
    22. WNT5B gene may contribute to conferring susceptibility to type 2 diabetes and may be involved in the pathogenesis of this disease through the regulation of adipocyte function. PMID: 15386214
    23. In leiomyoma cells, the regulation of Wnt5b expression by retinoids appears to be attenuated. PMID: 15972578
    24. Variation in WNT5B predisposes to type 2 diabetes in the absence of obesity. PMID: 18555673

    顯示更多

    收起更多

  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Wnt family
  • 數據庫鏈接：

    HGNC: 16265

    OMIM: 606361

    KEGG: hsa:81029

    STRING: 9606.ENSP00000308887

    UniGene: Hs.306051



主站蜘蛛池模板： 日韩乱码人妻无码系列中文字幕 | 亚洲精品国产成人| 精品日本一区二区三区在线观看| 久久精品无码一区二区日韩av| 精品午夜国产福利观看| 欧美高清大屁股xxxxx| 亚洲aⅴ男人的天堂在线观看 | 国产av熟女一区二区三区| 凹凸精品熟女在线观看| 男人天堂2018亚洲男人天堂| 日韩~欧美一中文字幕| 40岁成熟女人牲交片20分钟| 好大好湿好硬顶到了好爽视频| 日韩人妻无码精品系列专区 | 上司人妻互换中文字幕| 久久久噜噜噜久久中文字幕色伊伊 | 尤物yw午夜国产精品大臿蕉| 欧美饥渴少妇xxxxx性| 用舌头去添高潮无码视频| 亚洲精品国产电影| 亚洲乱码卡一卡二卡| av无码天一区二区一三区| 熟女人妻大叫粗大受不了| 国产男生午夜福利免费网站| av无码午夜福利一区二区三区| 精品久久久无码中文字幕| 亚洲欧美日韩国产成人| 天堂在线www资源在线| 亚洲精品中文字幕| 亚洲18色成人网站www| 狠狠色综合久久丁香婷婷| 精品国产高清毛片a片看| 亚洲精品一区二区三区四区五区| 亚洲 日韩 国产 中文有码| 无码人妻在线一区二区三区免费| 无码毛片内射白浆视频| 日本在线看片免费人成视频1000| 国产69精品久久久久乱码| 天天狠天天透天天伊人| 国产精品久久久久久影视| 在线精品国精品国产尤物|