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Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit

  • 中文名稱:
    人凝血酶原前體蛋白(PIVKA-II)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E13343h
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人凝血酶原前體蛋白(PIVKA-II)酶聯(lián)免疫試劑盒(CSB-E13343h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、腹水、組織勻漿樣本中的PIVKA-II含量。PIVKA-II即異常凝血酶原,是一種腫瘤標(biāo)志物。在維生素K缺乏或拮抗時產(chǎn)生,在肝癌等腫瘤細(xì)胞中高表達(dá)。研究機(jī)制上,它可反映肝細(xì)胞癌的生長和凝血功能異常,為肝癌早期診斷、病情監(jiān)測和預(yù)后評估提供重要參考。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,適用于肝癌發(fā)生機(jī)制研究、抗腫瘤藥物研發(fā)、肝臟功能評估等科研領(lǐng)域,其多樣本兼容性特別適合臨床前研究中不同生物樣本的橫向?qū)Ρ确治觯缒[瘤組織勻漿中異常凝血酶原表達(dá)檢測,或腹水樣本中的腫瘤標(biāo)志物水平動態(tài)監(jiān)測,為維生素K代謝研究及肝病相關(guān)分子機(jī)制探索提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    PIVKA-II
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, ascitic fluid, tissue homogenates
  • 檢測范圍:
    0.312 ng/mL-20 ng/mL
  • 靈敏度:
    0.078 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PIVKA-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-101  
    1:2 Average % 97  
    Range % 86-101  
    1:4 Average % 96  
    Range % 90-108  
    1:8 Average % 94  
    Range % 82-103  
  • 回收率:
    The recovery of human PIVKA-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-104  
    EDTA plasma (n=4) 97 91-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.467 2.418 2.443 2.327  
    10 1.962 1.945 1.954 1.838  
    5 1.342 1.311 1.327 1.211  
    2.5 0.823 0.772 0.798 0.682  
    1.25 0.504 0.496 0.500 0.384  
    0.625 0.331 0.321 0.326 0.210  
    0.312 0.201 0.189 0.195 0.079  
    0 0.117 0.114 0.116    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評價

相關(guān)問答

 常見問題解答
Q:

I have a question regarding calibration standard in the kit. How this standard protein synthesized or extracted from some source? and how many des-carboxy forms (PIVKAII) it includes?

A:
Thanks for your inquiry.
CSB-E13343h
PIVKA-II, also known as de-gamma-carboxyl prothrombin (DCP), is an abnormal form of prothrombin.
The standard is a special protein. Sorry that the specific preparation method is confidential and we can not disclose it. The standard is provided in the form of lyophilized powder, and the corresponding protective components will be added in it. The concentration is 20 ng/ml after re-suspension.Pls let me know if you have any further questions. Thank you.
Q:

Can you please let me know the below information:
1) Does the kit contain any human derived material? If so, please describe what it is.
2) Does the kit contain any animal derived material? If so please describe what it is. Please let us know if it contains polyclonal or monoclonal antibodies and what species the antibodies are from?

A:
Thanks for your inquiry. CSB-E13343h
Some components in the kit contain BSA.
There are 2 antibodies in the kit. Capture antibody is mouse monoclonal antibody and detection antibody is rabbit polyclonal antibody.Pls let me know if you have any further questions. Thank you.


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