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  4. Human Protein FAM13A(FAM13A) ELISA kit

Human Protein FAM13A(FAM13A) ELISA kit

  • 中文名稱：
    人FAM13A蛋白(FAM13A)酶聯免疫試劑盒
  • 貨號：
    CSB-EL008075HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人FAM13A蛋白(FAM13A)酶聯免疫試劑盒（CSB-EL008075HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FAM13A含量。試劑盒檢測范圍為23.44 pg/mL-1500 pg/mL，適用于體外研究FAM13A在疾病模型中的表達變化、分子機制探索及生物標志物篩選，如細胞實驗后的培養上清液檢測、動物模型組織樣本分析或臨床前研究中的體液樣本定量，為呼吸道疾病、代謝綜合征等領域的科研工作提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    FAM13A ELISA Kit; FAM13A1 ELISA Kit; KIAA0914Protein FAM13A ELISA Kit
  • 縮寫：
    FAM13A
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.44 pg/mL-1500 pg/mL
  • 靈敏度：
    5.86 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FAM13A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 88-105
    1:2 Average % 103
    Range % 97-108
    1:4 Average % 94
    Range % 88-99
    1:8 Average % 105
    Range % 94-108
  • 回收率：
    The recovery of human FAM13A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 94-104
    EDTA plasma (n=4) 102 94-107
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.467 2.402 2.435 2.299
    750 1.699 1.619 1.659 1.523
    375 1.045 1.073 1.059 0.923
    187.5 0.585 0.546 0.566 0.430
    93.75 0.378 0.355 0.367 0.231
    46.88 0.238 0.231 0.235 0.099
    23.44 0.213 0.220 0.217 0.081
    0 0.138 0.134 0.136 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

Per customers request, please provide the following information regarding Human Protein FAM13A, FAM13A ELISA Kit (CSB-EL008075HU)
1. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
2. Is the capture antibody a monoclonal or polyclonal?
3. What is the host of the capture antibody?
4. What’s the immunogen or immunogen range of the capture antibody?
5. What’s is the species of the immunogen of the capture antibody, human or something else?
6. Is the detection antibody a monoclonal or polyclonal?
7. What is the host of the detection antibody?
8. What is the immunogen or immunogen range of the detection antibody?
9. What is the species of the immunogen of the detection antibody? Human or something else?
10. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
11. If recombinant protein, is it full length or partial?
12. What is the species of the standard– human or something else?
13. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

A:
Thanks for your inquiry!
For Protein FAM13A, the protein sequence and the structure are different for different species.
CSB-EL008075HU
This kit is designed according to: https://www.uniprot.org/uniprot/O94988. It is specific to human.
Capture antibody is mouse monoclonal antibody and immunogen is NS0-derived recombinant Human Protein FAM13A protein.
Detection antibody is goat polyclonal antibody and immunogen is NS0-derived recombinant Human Protein FAM13A protein.
Standard is NS0-derived recombinant Human Protein FAM13A protein.
We tested undiluted serum and plasma samples before and the test value is 150--400pg/ml.
Pls let me know if you have any further questions. Thank you.


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