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  4. Human Prostasin(PRSS8) ELISA kit

Human Prostasin(PRSS8) ELISA kit

  • 中文名稱：
    人絲氨酸蛋白酶8(PRSS8)酶聯免疫試劑盒
  • 貨號：
    CSB-EL018825HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人絲氨酸蛋白酶8(PRSS8)酶聯免疫試劑盒（CSB-EL018825HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、唾液、組織勻漿樣本中的PRSS8含量。PRSS8是重要靶點。它在細胞生理活動、組織穩態維持中起關鍵作用。研究顯示其參與多種信號通路，與細胞增殖、遷移等密切相關。深入探究其作用機制，有助于揭示疾病發病機理，為開發針對性藥物及治療策略提供依據。試劑盒檢測范圍為2.35 ng/mL-150 ng/mL，該產品可廣泛應用于基礎研究領域，如探索PRSS8在皮膚疾病、呼吸道黏膜功能異常或泌尿系統相關病理過程中的作用機制，也可用于體外模型或動物實驗中蛋白表達水平的動態監測，為細胞生物學、分子病理學等研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    PRSS8; Prostasin; Channel-activating protease 1; CAP1; Serine protease 8
  • 縮寫：
    PRSS8
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, urine, saliva, tissue homogenates
  • 檢測范圍：
    2.35 ng/mL-150 ng/mL
  • 靈敏度：
    0.58 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 84  
    Range % 80-92  
    1:400 Average % 95  
    Range % 91-105  
    1:800 Average % 97  
    Range % 92-108  
    1:1600 Average % 93  
    Range % 86-100  
  • 回收率：
    The recovery of human PRSS8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 85-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    150 2.800 2.790 2.795 2.650  
    75 2.309 2.298 2.304 2.159  
    37.5 1.281 1.300 1.291 1.146  
    18.75 0.650 0.698 0.674 0.529  
    9.4 0.454 0.493 0.474 0.329  
    4.7 0.251 0.270 0.261 0.116  
    2.35 0.174 0.168 0.171 0.026  
    0 0.144 0.146 0.145 　  
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).
  • 基因功能參考文獻：
    1. limited role for HAI-2 in the inhibition of matriptase and prostasin is the result of its primarily intracellular localization in basal and spinous layer keratinocytes, which probably prevents the Kunitz inhibitor from interacting with active prostasin or matriptase PMID: 29438412
    2. PRSS8 was downregulated in glioma tissues.PRSS8 role in the glioma cell proliferation, migration, and invasion. PMID: 27983922
    3. PRSS8 acts as a tumor suppressor in colorectal cancer PMID: 27050145
    4. Data show that PRSS8 was reduced in esophageal squamous cell carcinomas (ESCC) tissues and the reduction of PRSS8 was associated with poor differentiation and shorter survival time. PMID: 27081034
    5. Studies in colonic T84 cell monolayers revealed that barrier disruption by the colitis-associated Th2-type cytokines, IL-4 and IL-13, down-regulates matriptase as well as prostasin through phosphorylation of the transcriptional regulator STAT6 PMID: 28490634
    6. PRSS8 may serve as a tumour suppressor in hepatocellular carcinoma progression. PMID: 27915333
    7. Overexpression of PRSS8 mRNA and high levels of prostasin in multiple subtypes of early stage ovarian tumors may provide clinical biomarkers for early detection of ovarian cancer. PMID: 27036110
    8. It is concluded that prostasin protein level change is less likely to be causally involved in placental dysfunction in preeclampsia. PMID: 26867056
    9. In urinary exosomes, NCC and prostasin had a diurnal pattern parallel to ADH and aquaporin 2, confirming that, in healthy subjects, both prostasin and NCC relate to water balance. PMID: 25931204
    10. Polymorphism of the prostasin gene is closely associated with poor pregnancy outcomes of early-onset severe preeclampsia. PMID: 26252104
    11. Prostasin regulate the Lin28/Let-7 loop in ovarian cancer cells. PMID: 25188517
    12. The higher frequency of C allele of prostasin gene at rs12597511 is associated with severe preeclampsia. PMID: 24890150
    13. Prostasin repress cancer cells and contribute to chemoresistance by modulating the CASP/PAK2-p34/actin pathway. PMID: 24434518
    14. Our study suggested that Axl and prostasin expression may be closely related to carcinogenesis, metastasis, and prognosis of ovarian adenocarcinoma. PMID: 23707658
    15. urinary prostasin correlates with the aldosterone to renin ratio, and it is physiologically modulated by natriuresis in normotensive individuals PMID: 23344129
    16. prostasin is a non-enzymatic co-factor for matriptase activation PMID: 23673661
    17. Prostasin requires expression of endogenous matriptase to stimulate barrier formation since matriptase depletion by siRNA silencing abrogates prostasin barrier-forming activity. PMID: 23443662
    18. Essential hypertension in Xinjiang Kazakhs is not associated with the 2827C>T polymorphism in the prostasin gene. PMID: 20078940
    19. prostasin is present, mature and active on the apical surface of wild-type and cystic fibrosis bronchial epithelial cells PMID: 22582115
    20. Study does not provide evidence of a major role of prostasin variation in blood pressure modulation. PMID: 21933610
    21. Expression of matriptase and prostasin is closely correlated in breast cancer cell lines and in breast cancer tissues. Matriptase and prostasin display a near identical spatial expression pattern in the epithelial compartment of breast cancer tissue. PMID: 21678412
    22. Transport via the transcytotic pathway makes prostasin available as a substrate for matriptase. PMID: 21148558
    23. the matriptase-prostasin proteolytic cascade is tightly regulated by two mechanisms: 1) prostasin activation temporally coupled to matriptase autoactivation and 2) HAI-1 rapidly inhibiting not only active matriptase but also active prostasin PMID: 20696767
    24. prostasin is overexpressed in many epithelial ovarian cancers; a potential tumor marker PMID: 19606239
    25. Hepsin activates prostasin and cleaves the extracellular domain of the epidermal growth factor receptor. PMID: 19911255
    26. Luciferase assay using CYP11B2 promoter revealed that prostasin significantly increased the transcriptional activity of CYP11B2. PMID: 20204133
    27. Prostasin may regulate trophoblast cell proliferation via modulating the EGFR-MAPK signaling pathway. PMID: 20089521
    28. In the in vitro cultured human choriocarcinomal JEG-3 cells, treatment with functional antibody against prostasin led to promotion in cell invasion capability, as well as increase in the production of MMP-2, MMP-26, TIMP-1, and TIMP-4. PMID: 19847458
    29. Loss of prostasin expression in bladder transitional cell carcinomas is associated with epithelial-mesenchymal transition. PMID: 19849847
    30. protease-mediated regulation of sodium absorption is a function of human airway epithelia, and prostasin is a likely candidate for this activity. PMID: 11756432
    31. prostasin is a major regulator of ENaC-mediated Na+ current in DeltaF508 cystic fibrosis epithelia PMID: 15246975
    32. prostasin is a channel activating protease; analysis of its substrate specificity PMID: 15474520
    33. HAI-1B is a potential physiological regulator of prostasin function PMID: 16103126
    34. Dihydrotestosterone regulates prostasin expression in prostate cells via sterol-regulatory element-binding protein stimulation and SLUG repression of prostasin promoter PMID: 16541421
    35. The PRSS8 gene was shown to effectively separate these two tumor(chromophobe renal cell carcinoma (RCC) and benign oncocytoma) groups by quantitative reverse transcription-PCR using fresh tissue samples, with similar trends seen on formalin-fixed tissues. PMID: 17145811
    36. Data show that prostasin induces protease-dependent and independent molecular changes in the human prostate carcinoma cell line PC-3. PMID: 17532063
    37. reduced activity of a matriptase-prostasin proteolytic cascade is the etiological origin of human ARIH and provides an important mouse model for the exploration of matriptase function in ARIH PMID: 17940283
    38. prostasin, an upstream prostasin-activating protease, and PN-1 regulate Na+ absorption in the airway and that abnormal prostasin expression contributes to excessive proteolytic activation of ENaC in cystic fibrosis patients PMID: 18310226
    39. Genetic variation of the prostasin gene may be implicated in the development of hypertension in youths. PMID: 18583984
    40. crystallographic analysis of prostasin PMID: 18922802
    41. Suggest that urinary prostasin could be a novel biomarker and/or mechanism for renal pressure natriuresis in normotensive black adolescents. PMID: 19127211
    42. it is not clear, at the present time, whether endogenous aldosterone regulates prostasin expression or vice versa PMID: 19262497
    43. study presents structures of the extracellular domain of active prostasin in several forms; of particular interest is the S1 subsite loop which opens and closes in response to basic residues or divalent ions, directly binding Ca(+2) cations PMID: 19388054
    44. the mRNA level of PN-1 is significantly elevated in colorectal cancer tissue PMID: 19555470

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  • 亞細胞定位：
    [Prostasin]: Cell membrane; Single-pass membrane protein.; [Prostasin light chain]: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus.; [Prostasin heavy chain]: Secreted, extracellular space. Note=Found in the seminal fluid. Secreted after cleavage of its C-terminus.
  • 蛋白家族：
    Peptidase S1 family
  • 組織特異性：
    Found in prostate, liver, salivary gland, kidney, lung, pancreas, colon, bronchus and renal proximal tubular cells. In the prostate gland it may be synthesized in epithelial cells, secreted into the ducts, and excreted into the seminal fluid.
  • 數據庫鏈接：

    HGNC: 9491

    OMIM: 600823

    KEGG: hsa:5652

    STRING: 9606.ENSP00000319730

    UniGene: Hs.75799



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