Human Prostaglandin F2α,PGF2α ELISA Kit
-
中文名稱:人前列腺素F2α(PGF2α)酶聯(lián)免疫試劑盒
-
貨號(hào):CSB-E10142h
-
規(guī)格:96T/48T
-
價(jià)格:¥3600/¥2500
-
其他:
產(chǎn)品詳情
-
產(chǎn)品描述:人前列腺素F2α(PGF2α)酶聯(lián)免疫試劑盒(CSB-E10142h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的PGF2α含量。PGF2α 是重要靶點(diǎn)。它作為前列腺素家族一員,在人體生理過程如生殖、心血管調(diào)節(jié)等方面發(fā)揮作用。研究機(jī)制上,其通過與特定受體結(jié)合,激活下游信號(hào)通路,影響細(xì)胞功能和生理反應(yīng),在相關(guān)疾病治療領(lǐng)域具潛在研究?jī)r(jià)值。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL,該產(chǎn)品適用于探索PGF2α在生殖系統(tǒng)模型(如前列腺功能研究)、心血管疾病機(jī)制、炎癥反應(yīng)調(diào)控及藥物干預(yù)實(shí)驗(yàn)中的生物學(xué)作用,為細(xì)胞信號(hào)通路研究、疾病分子機(jī)制解析及潛在生物標(biāo)志物開發(fā)提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
-
別名:PGF2 alpha,Prostaglandin F2 alpha
-
縮寫:PGF2α
-
種屬:Homo sapiens (Human)
-
樣本類型:serum, plasma, tissue homogenates
-
檢測(cè)范圍:1.56 pg/mL-100 pg/mL
-
靈敏度:0.39 pg/mL
-
反應(yīng)時(shí)間:1-5h
-
樣本體積:50-100ul
-
檢測(cè)波長(zhǎng):450 nm
-
研究領(lǐng)域:Signal Transduction
-
測(cè)定原理:quantitative
-
測(cè)定方法:Sandwich
-
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human PGF2α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-98 1:2 Average % 102 Range % 97-107 1:4 Average % 93 Range % 85-99 1:8 Average % 98 Range % 91-104 -
回收率:
The recovery of human PGF2α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-99 EDTA plasma (n=4) 88 81-95 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 100 2.822 2.963 2.893 2.778 50 2.212 2.366 2.289 2.174 25 1.254 1.356 1.305 1.190 12.5 0.640 0.655 0.648 0.533 6.25 0.411 0.445 0.428 0.313 3.12 0.242 0.255 0.249 0.134 1.56 0.175 0.172 0.174 0.059 0 0.116 0.113 0.115 -
數(shù)據(jù)處理:
-
貨期:3-5 working days
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-