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Your Good Partner in Biology Research

  • 中文名稱(chēng):
    人前纖維蛋白1酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E16491h
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人前纖維蛋白1酶聯(lián)免疫試劑盒(CSB-E16491h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的PFN1含量。PFN1 即 Profilin 1,是一種在細(xì)胞內(nèi)廣泛分布的肌動(dòng)蛋白結(jié)合蛋白。它可調(diào)節(jié)肌動(dòng)蛋白聚合,在細(xì)胞遷移、增殖、存活等方面發(fā)揮關(guān)鍵作用。當(dāng)前針對(duì) PFN1 在腫瘤發(fā)生發(fā)展、神經(jīng)退行性疾病等機(jī)制中的研究較多,以期為相關(guān)疾病治療提供新靶點(diǎn)。試劑盒檢測(cè)范圍為0.78 ng/ml- 50 ng/ml,適用于細(xì)胞生物學(xué)、病理機(jī)制等科研領(lǐng)域的研究;可為探究Profilin 1在疾病模型中的功能、信號(hào)通路交互作用以及生物標(biāo)志物篩選提供可靠工具,特別適用于腫瘤生物學(xué)、炎癥反應(yīng)或神經(jīng)退行性疾病等相關(guān)課題的體外實(shí)驗(yàn)研究。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Actin binding protein ELISA Kit; ALS18 ELISA Kit; Epididymis tissue protein Li 184a ELISA Kit; OTTHUMP00000125244 ELISA Kit; PFN 1 ELISA Kit; Pfn ELISA Kit; PFN1 ELISA Kit; PROF1_HUMAN ELISA Kit; Profilin I ELISA Kit; Profilin-1 ELISA Kit; Profilin1 ELISA Kit; ProfilinI ELISA Kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.78 ng/ml - 50 ng/ml
  • 靈敏度:
    0.195 ng/ml
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human profilin 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    106

    Range %

    100-111

    1:2

    Average %

    96

    Range %

    89-103

    1:4

    Average %

    99

    Range %

    92-105

    1:8

    Average %

    102

    Range %

    97-105

  • 回收率:

    The recovery of human profilin 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    91-100

    EDTA plasma (n=4)

    90

    86-97

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    50

    2.539

    2.634

    2.587

    2.455

    25

    1.835

    1.767

    1.801

    1.670

    12.5

    1.199

    1.158

    1.178

    1.047

    6.25

    0.732

    0.729

    0.731

    0.599

    3.12

    0.452

    0.431

    0.442

    0.310

    1.56

    0.297

    0.303

    0.300

    0.168

    0.78

    0.215

    0.209

    0.212

    0.081

    0

    0.134

    0.129

    0.132

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

May I know the antibody information of this kit?

A:
Thanks for your inquiry. The antibody information is:
Coated anti: Mouse monoclonal antibody
Detection anti: Goat polyclonal antibody
Pls let me know if you have any further questions. Thank you.

靶點(diǎn)詳情

  • 功能:
    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.
  • 基因功能參考文獻(xiàn):
    1. Variants of rs238243 and rs238238 might regulate profilin1 expression by epigenetic modification and indirectly affects the susceptible threshold of hypertension in Chinese Han population. PMID: 28541412
    2. Results suggested that the RhoA/ROCK1 pathway activated by excessive ROS is responsible for profilin-1-mediated endothelial damage. PMID: 29849894
    3. PFN1 could promote autophagy through taking part in Beclin1 complex and contribute to bortezomib resistance, which may become a novel molecular target in the therapy of MM. PMID: 29945297
    4. Loss of PFN1 in tumor cells has been associated with lymph node invasion and metastasis in other tumor types, strengthening the argument that the protein has the potential to be a tumor suppressor in late-stage oral squamous cell carcinoma. PMID: 27862305
    5. Guttiferone K effectively suppresses the motility and metastasis of hepatocellular carcinoma cells mainly by restoration of aberrantly reduced PFN1 protein expression PMID: 27494863
    6. Results collectively suggest that PFN1 promotes cell migration and adhesion in bladder cancer models. PMID: 27683119
    7. These results suggest that although mutant PFN1 aggregation may contribute to neurodegeneration, it does not trigger its onset. Importantly, these experiments establish a progressive disease model that can contribute toward identifying the mechanisms of ALS pathogenesis and the development of therapeutic treatments. PMID: 27681617
    8. One potential mechanism for C71G-PFN1 to initiate Amyotrophic lateral sclerosis might be the abnormal interaction with membranes as recently established for SOD1 mutants. PMID: 28847504
    9. Expression of PFN1 mutants induces accumulation of TDP-43, and promotes conversion of normal TDP-43 into an abnormal form. These results provide new insight into the mechanisms of TDP-43 proteinopathies and other diseases associated with amyloid-like protein deposition. PMID: 27432186
    10. We suggest that reduction of PFN-1 expression by elevated levels of PrP(c) may contribute to protective effects PrP(c)-overexpressing SH-SY5Y cells confer against STS-induced apoptosis PMID: 28102851
    11. this study shows that in pancreatic cancer patients, PFN1 expression is substantially decreased in peripheral CD8(+) T cells PMID: 28688208
    12. mutant profilin1 in various diseases with an emphasis on its contribution to the pathogenesis of amyotrophic lateral sclerosis (Review) PMID: 27669692
    13. Data suggest 2 major isoforms of profilin (Pfn1 and Pfn2) are co-regulated by a common mechanism involving the action of MKL1 [megakaryoblastic leukemia (translocation) 1 protein] that is independent of its SRF- (serum-response factor)-related activity; cellular externalization of Pfn1, rather than transcription, is affected by the perturbations of MKL1; MKL1 can influence cell migration by modulating Pfn1 expression. PMID: 28546428
    14. novel profilin-1 variants associated with amyotrophic lateral sclerosis PMID: 27101547
    15. We found that ARP3 and profilin1 were 2 binding partners of LMO2, primarily in cytoplasm. LMO2. LMO2 mediated the assembly of a complex including ARP3, profilin1, and actin monomer, increased actin monomer binding to profilin1, and promoted lamellipodia/filopodia formation in basal-type breast cancer cells. PMID: 28170369
    16. These observations indicate that our novel profilin1 mutant mouse line may provide a new ALS model with the opportunity to gain unique perspectives into mechanisms of neurodegeneration that contribute to ALS pathogenesis. PMID: 28040732
    17. These data suggest that Familial Amyotrophic Lateral Sclerosis-linked PFN1 mutations exacerbate TDP-43-induced neurodegeneration in a gain-of-function manner, possibly by shifting the localization of TDP-43 from nuclei to cytoplasm. PMID: 27634045
    18. Homo-oligomerization of the actin-binding protein PFN1 has been characterized by the relaxation dispersion profiles of the protein as a function of concentration. PMID: 28052669
    19. Gain-of-toxic-function PFN1 gene mutation leads to conformational change of TDP-43 and to neurodegeneration in amyotrophic lateral sclerosis. PMID: 26908597
    20. Profilin synergizes with chemotherapeutic drugs to induce tumor cell death by regulating NF-kappaB and p53. Thus, modulation of Profilin may be a useful strategy for effective combination therapy. PMID: 26842845
    21. Mutations of profilin-1, associated with familial amyotrophic lateral sclerosis, increase the tendency of profilin-1 to aggregate and that such aggregation behavior is largely determined by the mutation-induced structural changes occurring in the folded state of the protein. PMID: 26226631
    22. evidence, which suggests that Profilin increases tumour suppressor activity by regulating NF-kappaB. PMID: 26787927
    23. Profilin-1 folding process occurs in the absence of thermodynamically stable partially folded states. PMID: 26227615
    24. Actin independent mechanisms contribute to the pathogenicity of PFN1 T109M and possibly other PFN1 mutations. PMID: 26572741
    25. expression of the ALS-associated actin-binding deficient mutant of PFN1 (PFN1(C71G)) results in increased dendritic arborisation and spine formation, and cytoplasmic inclusions in cultured mouse hippocampal neurons PMID: 26499959
    26. PFN1 is a rare cause of ALS. PMID: 25499087
    27. findings suggest that a destabilized form of PFN1 underlies PFN1-mediated ALS pathogenesis PMID: 26056300
    28. Suggest that PFN1 plays a critical role in gastric carcinoma progression, and these effects are likely mediated through the integrin beta1/FAK pathway. PMID: 25741138
    29. Data indicated that No PFN1 mutations were identified in the Catalan population with amyotrophic lateral sclerosis. PMID: 25249294
    30. Profilin-1 overexpression in MDA-MB-231 breast cancer cells is associated with alterations in proteomics biomarkers of cell proliferation, survival, and motility as revealed by global proteomics analyses PMID: 25454514
    31. Profilin1 acts as a molecular regulator of the levels of PI(3,4)P2 and Tks5 recruitment in invadopodia to control the invasion efficiency of invadopodia. PMID: 25613364
    32. Collective expression pattern of tensin/profilin-1/villin-1/talin could be a biomarker to estimate the prognosis of esophageal squamous cell carcinoma patients. PMID: 25337239
    33. Pfn1 is a tumor suppressor in pancreatic cancer that acts via a novel mechanism of regulating the SIRT3-HIF1alpha axis. PMID: 25103363
    34. Higher messenger RNA expression of Profilin-1 is associated with significantly lower survival PMID: 25704627
    35. the exchange of bound actin between Tbeta4 and profilin involves both steric and allosteric components. PMID: 25313062
    36. effects of profilin-1 and profilin-2, the two major isoforms of profilin, on actin cytoskeletal regulation, motility, and invasion of breast cancer cells PMID: 23827010
    37. This review summarize the PFN1 most recently discovered 'high risk' genes in ALS. PMID: 24780888
    38. association of cortactin with Pfn-1 is regulated by c-Abl-mediated cortactin phosphorylation PMID: 24700464
    39. Raising the intracellular levels of Profilin I decreased the mobile fraction ratio of actin filaments and slowed their polymerization rate. PMID: 24465723
    40. In glioblastomas endothelial cell-specific Pfn-1 phosphorylation elevates HIF-1alpha expression leading to vascular abnormalities and tumor progression. PMID: 24747440
    41. The ALS-linked mutations in profilin 1 alter stress granule dynamics, providing further evidence for the potential role of stress granules in ALS pathogenesis. PMID: 24920614
    42. CHIP regulates Pfn1 levels as an E3 ligase, and possibly plays a role in cell migration and metastasis of breast cancer. PMID: 24661873
    43. Data indicate that lower profilin1 (Pfn1) expression is associated with increased metastatic potential in breast cancer. PMID: 23686314
    44. PFN1 mutations were identified in autosomal dominant FALS patients. PMID: 24085347
    45. Profilin-1 might act as an ultimate and common cellular effector in the process of metabolic memory (endothelial abnormalities) mediated by AGEs via the ROS/PKC or ROS/NF-B signalling pathways. PMID: 24090212
    46. Wanted to identify estrogen receptor alpha (ERalpha) interacting proteins in Tamoxifen treated MCF7 cells. Using a GST-pull down assay with ERalpha ligand-binding domain and MS-based proteomics approach we identified Profilin1 as a novel ERalpha interacting protein. PMID: 23576398
    47. up-regulation of profilin1 facilitated apoptosis and repressed autophagy induced by irradiation. PMID: 23826918
    48. PFN1 mutations lead to ubiquitin-positive inclusions and impairment of cytoskeletal pathways, in which, a pathophysioloy of familial and sporadic ALS lays. PMID: 23635659
    49. The single nucleotide polymorphism (SNP) rs13204 of the PFN1 gene has an important function in the development of amyotrophic lateral sclerosis in Han Chinese. PMID: 23428184
    50. PFN1 mutations are not a common cause of frontotemporal lobar degeneration and amyotrophic lateral sclerosis in this cohort of patients from France. PMID: 23182804

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  • 相關(guān)疾病:
    Amyotrophic lateral sclerosis 18 (ALS18)
  • 亞細(xì)胞定位:
    Cytoplasm, cytoskeleton.
  • 蛋白家族:
    Profilin family
  • 組織特異性:
    Expressed in epididymis (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接:

    HGNC: 8881

    OMIM: 176610

    KEGG: hsa:5216

    STRING: 9606.ENSP00000225655

    UniGene: Hs.494691



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