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  4. Human Polycomb complex protein BMI-1(BMI1) ELISA kit

Human Polycomb complex protein BMI-1(BMI1) ELISA kit

  • 中文名稱：
    人多梳復(fù)合蛋白BMI-1(BMI1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL002726HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人多梳復(fù)合蛋白BMI-1(BMI1)酶聯(lián)免疫試劑盒（CSB-EL002726HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的BMI1含量。BMI1是一個重要靶點(diǎn)。它是原癌基因，在調(diào)節(jié)細(xì)胞增殖、分化、衰老及凋亡等方面起關(guān)鍵作用。研究機(jī)制上，它參與調(diào)控多條重要信號通路，如抑制p16等腫瘤抑制基因表達(dá)。異常表達(dá)與多種腫瘤發(fā)生、發(fā)展密切相關(guān)，成為抗癌藥物研發(fā)焦點(diǎn)。試劑盒檢測范圍為25 pg/mL-1600 pg/mL，適用于腫瘤生物學(xué)、干細(xì)胞分化等基礎(chǔ)研究領(lǐng)域，可精準(zhǔn)檢測不同實驗條件下BMI1的蛋白表達(dá)水平變化，為探索腫瘤發(fā)生機(jī)制、評估靶向藥物干預(yù)效果提供可靠工具，廣泛適用于細(xì)胞模型、動物模型及臨床前樣本的蛋白定量分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    B lymphoma Mo MLV insertion region (mouse) ELISA Kit; B lymphoma Mo MLV insertion region 1 homolog ELISA Kit; Bmi 1 ELISA Kit; BMI1 ELISA Kit; BMI1 polycomb ring finger oncogene ELISA Kit; BMI1_HUMAN ELISA Kit; Flvi 2/bmi 1 ELISA Kit; FLVI2/BMI1 ELISA Kit; MGC12685 ELISA Kit; Murine leukemia viral (bmi 1) oncogene homolog ELISA Kit; Oncogene BMI 1 ELISA Kit; PCGF 4 ELISA Kit; PCGF4 ELISA Kit; Polycomb complex protein BMI 1 ELISA Kit; Polycomb complex protein BMI-1 ELISA Kit; Polycomb group protein Bmi1 ELISA Kit; Polycomb group ring finger 4 ELISA Kit; Polycomb group RING finger protein 4 ELISA Kit; RING finger protein 51 ELISA Kit; RNF 51 ELISA Kit; RNF51 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    25 pg/mL-1600 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human BMI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 84-93
    1:2 Average % 95
    Range % 90-100
    1:4 Average % 103
    Range % 99-107
    1:8 Average % 90
    Range % 85-95
  • 回收率：
    The recovery of human BMI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 85 80-89
    EDTA plasma (n=4) 94 90-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 1.768 1.896 1.832 1.714
    800 1.169 1.203 1.186 1.068
    400 0.739 0.704 0.722 0.604
    200 0.472 0.457 0.465 0.347
    100 0.320 0.340 0.330 0.212
    50 0.267 0.271 0.269 0.151
    25 0.195 0.182 0.189 0.071
    0 0.121 0.115 0.118 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 1066

    OMIM: 164831

    KEGG: hsa:100532731

    STRING: 9606.ENSP00000365851

    UniGene: Hs.380403



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