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  4. Human Plexin-C1(PLXNC1) ELISA kit

Human Plexin-C1(PLXNC1) ELISA kit

  • 中文名稱：
    人叢狀蛋白C1(PLXNC1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL018225HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人叢狀蛋白C1(PLXNC1)酶聯免疫試劑盒（CSB-EL018225HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PLXNC1含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于探索PLXNC1在神經退行性疾病模型中的功能機制、腫瘤微環境相互作用研究，或作為炎癥相關信號通路研究的工具，為神經科學、免疫學及癌癥生物學領域的體外實驗提供可靠的蛋白定量支持本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    CD232 ELISA Kit; Plexin-C1 ELISA Kit; PLXC1_HUMAN ELISA Kit; Plxnc1 ELISA Kit; Virus-encoded semaphorin protein receptor ELISA Kit
  • 縮寫：
    PLXNC1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PLXNC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %92-98
    1:2Average %93
    Range %85-97
    1:4Average %98
    Range %94-102
    1:8Average %85
    Range %80-89
  • 回收率：
    The recovery of human PLXNC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10497-108
    EDTA plasma (n=4)8782-93
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.016 2.118 2.067 1.873
    101.735 1.637 1.686 1.492
    51.164 1.226 1.195 1.001
    2.50.753 0.775 0.764 0.570
    1.250.552 0.543 0.548 0.354
    0.6250.413 0.405 0.409 0.215
    0.3120.312 0.304 0.308 0.114
    00.193 0.195 0.194
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8.
  • 基因功能參考文獻：
    1. Results indicate that long non-coding RNA (lncRNA) CASC2 can promote PLXNC1 expression by sponging miR-181a, thereby inhibiting the proliferation and invasion of melanoma cells. PMID: 29514220
    2. Plexin C1 deficiency permits synaptotagmin 7-mediated macrophage migration and enhances mammalian lung fibrosis PMID: 27609773
    3. loss of Plexin C1 in melanoma may promote early steps in melanoma progression through suppression of migration and proliferation PMID: 23160370
    4. Study reports the structures of Sema7A and A39R complexed with the Semaphorin-binding module of PlexinC1; both structures show two PlexinC1 molecules symmetrically bridged by Semaphorin dimers. PMID: 20727575
    5. GTP-bound form of Rab7 promotes melanogenesis through the regulation of gp100 maturation in melanoma cells. PMID: 17671519
    6. Plexin C1, a receptor for semaphorin 7a, inactivates cofilin and is a potential tumor suppressor for melanoma progression. PMID: 18987670
    7. significant loss of Plexin C1 in metastatic melanoma compared with primary melanoma, suggesting the possibility that the Plexin C1 receptor is a tumor suppressor protein for melanoma PMID: 19318806

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  • 亞細胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Plexin family
  • 組織特異性：
    Detected in heart, brain, lung, spleen and placenta.
  • 數據庫鏈接：

    HGNC: 9106

    OMIM: 604259

    KEGG: hsa:10154

    STRING: 9606.ENSP00000258526

    UniGene: Hs.584845



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