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Human Plastin-2(LCP1) ELISA kit

  • 中文名稱:
    人網素-2(LCP1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL012824HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人網素-2(LCP1)酶聯免疫試劑盒(CSB-EL012824HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的LCP1含量。人網素-2(LCP1)是一種肌動蛋白結合調節因子,在T細胞激活中發揮關鍵作用,通過TCR/CD3和CD2或CD28響應共刺激信號,調節IL2RA/CD25和CD69的細胞表面表達,并參與免疫反應和T細胞激活。其研究機制涉及與AIF1和肌動蛋白的相互作用,以及調節細胞表面信號分子的表達。試劑盒檢測范圍為15.6 pg/ml-1000 pg/ml,該產品適用于基礎科研領域,如探究LCP1在免疫細胞功能、腫瘤微環境調控或炎癥性疾病模型中的表達特征,亦可結合其他分子標記物進行多維度機制研究,為體外實驗及動物模型樣本分析提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    CP64 ELISA Kit; L plastin ELISA Kit; L-plastin ELISA Kit; Larval cuticle protein 1 ELISA Kit; LC64P ELISA Kit; LCP-1 ELISA Kit; LCP1 ELISA Kit; LPL ELISA Kit; Lplastin ELISA Kit; Lymphocyte cytosolic protein 1 ELISA Kit; Plastin 2 ELISA Kit; Plastin-2 ELISA Kit; PLS2 ELISA Kit; PLSL_HUMAN ELISA Kit
  • 縮寫:
    LCP1
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates.
  • 檢測范圍:
    15.6 pg/ml-1000 pg/ml.
  • 靈敏度:
    3.9 pg/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human LCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    106

    Range %

    102-112

    1:2

    Average %

    96

    Range %

    90-102

    1:4

    Average %

    98

    Range %

    91-105

    1:8

    Average %

    91

    Range %

    87-96

  • 回收率:

    The recovery of human LCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    88-97

    EDTA plasma (n=4)

    99

    92-106

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    1.998

    1.874

    1.936

    1.813

    500

    1.264

    1.257

    1.260

    1.137

    250

    0.841

    0.828

    0.834

    0.711

    125

    0.518

    0.529

    0.523

    0.400

    62.5

    0.383

    0.391

    0.387

    0.264

    31.2

    0.295

    0.283

    0.289

    0.166

    15.6

    0.199

    0.189

    0.194

    0.071

    0

    0.122

    0.125

    0.123

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
  • 基因功能參考文獻:
    1. LCP1-positive oral squamous cell carcnome samples were correlated closely with the primary tumor size and regional lymph node metastasis. PMID: 28230172
    2. MOLP8/R cells display a very high overexpression of LCP1 gene (l-Plastin) controlled by HIF1&alpha. PMID: 29882856
    3. these findings support a plausible mechanism by which the AP4/L-plastin axis is regulated by the PI3K/AKT pathway in human prostate cancer (PCa)and may represent a novel therapeutic target in PCa treatment. PMID: 28981098
    4. Mutated LCP1 is a driver of chronic lymphocytic leukemia. PMID: 28679620
    5. AngII-dependent phosphorylation of LCP1 in cultured podocytes was mediated by the kinases ERK, p90 ribosomal S6 kinase, PKA, or PKC. LCP1 phosphorylation increased filopodia formation. PMID: 28768720
    6. L-plastin regulates the stability of the immune synapse of naive and effector T-cells. (Review) PMID: 27720134
    7. The findings support a mechanism in which miR-375 suppresses RUNX1 levels, resulting in reduced vimentin and L-plastin expression. Knockdown of RUNX1, L-plastin, and vimentin resulted in significant reductions in cell invasion in vitro, indicating the functional significance of miR-375 regulation of specific proteins involved in head and neck squamous cell carcinoma (HNSCC) invasion. PMID: 28499703
    8. In this study, the authors found that the actin filament bundling abilities of PLS1 and PLS2 were similarly sensitive to Ca(2+) (pCa50 ~6.4), whereas PLS3 was less sensitive (pCa50 ~5.9). PMID: 28694070
    9. elevated L-plastin expression promotes elongation and reduces protrusion density in cells with relatively lower L-plastin than fascin levels. PMID: 26945069
    10. Enhanced nitroxidative stress may results in LPL S-glutathionylation leading to impaired chemotaxis, polarization, and bactericidal activity of human neutrophils. PMID: 25881549
    11. association of SNPs in LCP1 and CTIF with hearing PMID: 26264041
    12. An NKX3.1 binding site polymorphism in the l-plastin promoter leads to differential gene expression in human prostate cancer PMID: 26148677
    13. The proteins (HSP90b, TSM1 and L-plastin) in the current study may hold potential in differentiating between melanoma and benign nevi in diagnostically challenging cases. PMID: 25191796
    14. Data suggest that several single-nucleotide polymorphisms (SNPs) of the plastin genes PLS3 and LCP1 could serve as gender- and/or stage-specific molecular predictors of tumor recurrence in stage II/III colorectal cancer as well as therapeutic targets. PMID: 24170770
    15. L-plastin plays an important role in the clustering of NKG2D into lipid rafts, and it participates in NKG2D-mediated inhibition of NK cell chemotaxis. PMID: 24803550
    16. expression of L-plastin promotes tumor metastasis and, importantly, that this effect depends on an additionally required phosphorylation of L-plastin PMID: 24438191
    17. L-plastin is indispensable for podosome formation and function in macrophages. PMID: 24236012
    18. LCP1 is functionally relevant to CXCL12 induced B-cell migration. PMID: 24009233
    19. High serum LCP1 is associated with kidney cancer. PMID: 23479363
    20. Hepatic LCP1 mRNA was increased (by 300%) in liver biopsy samples from patients with nonalcoholic fatty liver disease compared to controls PMID: 23213074
    21. This study adds L-plastin to a growing list of proteins implicated in T lymphocyte polarity and migration PMID: 22581862
    22. our data introduce costimulation-induced L-plastin phosphorylation as an important event for immune synapse formation and its inhibition by dexamethasone as a novel mode of function of this immunosuppressive glucocorticoid. PMID: 21805466
    23. Results establish a causative role for PKCbetaII and L-plastin in linking GM-CSF-induced eosinophil priming for chemotaxis. PMID: 21525390
    24. Plasmic L-plastin level in patients with colorectal cancer was higher than that in healthy adults, and was associated with tumor size, penetration, and lymphatic metastasis. PMID: 20878578
    25. required for immune synapse formation PMID: 20683899
    26. This study discloses a novel unexpected role of the actin bundling protein L-plastin as a cell protective protein against TNF-cytotoxicity. PMID: 19799649
    27. Data demonstrate for the first time that L-plastin contributes to the fine-tuning of actin turn-over, an activity which is regulated by Ser5 phosphorylation promoting its high affinity binding to the cytoskeleton. PMID: 20169155
    28. Data show that the serine protease plasmin cleaved both propeptides from human vascular endothelial growth factor (VEGF)-D, generating mature forms, and also activated VEGF-C. PMID: 12963694
    29. association of L-plastin overexpression with increased rate of proliferation and invasion, and loss of E-cadherin expression in the SW480 colon cancer cell line indicates that L-plastin plays an important mechanistic role in colorectal cancer metastasis PMID: 16287074
    30. Data suggest that phosphorylated L-plastin might act as an integrator of signals controlling the assembly of the actin cytoskeleton and cell motility in a 3D-space. PMID: 16636079
    31. Data show that an increase in melanoma cell invasiveness requires not only expression but also phosphorylation of L-plastin. PMID: 17290393
    32. Phosphorylation of the actin bundling protein L-plastin represents a mechanism by which costimulation controls the transport of activation receptors to the T cell surface. PMID: 17294403
    33. L-plastin and S100A9 were differentially expressed in nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissue PMID: 19142861

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  • 相關疾病:
    Chromosomal aberrations involving LCP1 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;13)(q27;q14), with BCL6.
  • 亞細胞定位:
    Cytoplasm, cytoskeleton. Cell junction. Cell projection. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
  • 組織特異性:
    Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
  • 數據庫鏈接:

    HGNC: 6528

    OMIM: 153430

    KEGG: hsa:3936

    STRING: 9606.ENSP00000315757

    UniGene: Hs.381099



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