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Human Pikachurin(EGFLAM) ELISA kit

  • 中文名稱:
    人皮卡丘素(EGFLAM)酶聯免疫試劑盒
  • 貨號:
    CSB-EL007478HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人皮卡丘素(EGFLAM)酶聯免疫試劑盒(CSB-EL007478HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的EGFLAM含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,該產品為科研領域研究者提供高效工具,支持細胞生物學實驗中的蛋白表達動態監測、疾病模型中的分子機制研究,以及生物樣本中EGFLAM的定量分析等應用場景本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Agrin-like protein ELISA Kit; AGRINL ELISA Kit; AGRNL ELISA Kit; EGF-like ELISA Kit; EGF-like; fibronectin type III and laminin G domains ELISA Kit; EGF-like; fibronectin type-III and laminin G-like domain-containing protein ELISA Kit; EGFLA_HUMAN ELISA Kit; EGFLAM ELISA Kit; fibronectin type-III and laminin G-like domain-containing protein ELISA Kit; FLJ39155 ELISA Kit; Pikachurin ELISA Kit
  • 縮寫:
    EGFLAM
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.81 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human EGFLAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %90-102
    1:2Average %85
    Range %81-92
    1:4Average %96
    Range %90-102
    1:8Average %93
    Range %87-99
  • 回收率:
    The recovery of human EGFLAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9895-104
    EDTA plasma (n=4)10298-106
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.717 2.787 2.752 2.644
    10002.120 2.160 2.140 2.032
    5001.468 1.425 1.447 1.339
    2500.798 0.769 0.784 0.676
    1250.469 0.457 0.463 0.355
    62.50.290 0.280 0.285 0.177
    31.250.212 0.225 0.219 0.111
    00.107 0.109 0.108  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.
  • 基因功能參考文獻:
    1. Strong candidate gene for macular dystrophy, MCDR3 (human 5p15.33-p13.1). Conclusion is based on a massive expression data set for mouse (103 strains) and joint analysis of RetNet database. PMID: 19727342
  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix. Cell junction, synapse, synaptic cleft. Cell junction, synapse, presynaptic active zone.
  • 數據庫鏈接:

    HGNC: 26810

    OMIM: 617683

    KEGG: hsa:133584

    STRING: 9606.ENSP00000346964

    UniGene: Hs.20103



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