Human Pepsinogen II ELISA Kit

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Human Pepsinogen II ELISA Kit

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文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

中文名稱(chēng)：

人胃蛋白酶原II酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E17539h
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

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人胃蛋白酶原II酶聯(lián)免疫試劑盒（CSB-E17539h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液樣本中的PG II含量。PG II即胃蛋白酶原II，是胃蛋白酶的前體。其背景在于與胃部健康密切相關(guān)，可反映胃黏膜狀態(tài)。研究機(jī)制方面，通過(guò)檢測(cè)血清中PG II水平，能輔助診斷胃部疾病，如胃潰瘍、胃癌等，為胃腸病的早期篩查提供重要依據(jù)。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL，適用于基礎(chǔ)醫(yī)學(xué)研究領(lǐng)域，包括胃黏膜功能評(píng)估、胃酸分泌調(diào)控機(jī)制研究、胃部疾病動(dòng)物模型構(gòu)建等科研場(chǎng)景，同時(shí)可應(yīng)用于體外培養(yǎng)細(xì)胞分泌功能檢測(cè)，為探索胃蛋白酶原II在消化系統(tǒng)疾病中的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

PG II
種屬：

Homo sapiens (Human)
樣本類(lèi)型：

serum, plasma, cell culture supernates
檢測(cè)范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	96-107
1:2	Average %	94
	Range %	90-98
1:4	Average %	95
	Range %	89-99
1:8	Average %	93
	Range %	87-98

回收率：

The recovery of human pepsinogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	86	81-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.624	2.733	2.679	2.505
200	2.189	2.077	2.133	1.959
100	1.425	1.353	1.389	1.215
50	0.746	0.735	0.741	0.567
25	0.437	0.425	0.431	0.257
12.5	0.312	0.326	0.319	0.145
6.25	0.238	0.234	0.236	0.062
0	0.175	0.173	0.174

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Helicobacter pylori Stool Antigen Levels and Serological Biomarkers of Gastric Inflammation are Associated with Cardio-Metabolic Risk Factors in Type 2 Diabetic Patients. Bahadoran Z.et al,Endocrinol Metab (Seoul).,2015
Complementary and alternative medicinal effects of broccoli sprouts powder on Helicobacter pylori eradication rate in type 2 diabetic patients: A randomized Zahra Bahadoran et al,Journal of Functional Foods,2014

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.