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  4. Human Paraoxonase 3 (PON3) ELISA Kit

Human Paraoxonase 3 (PON3) ELISA Kit

  • 中文名稱：
    人對氧磷酶-3(PON3)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E15783h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人對氧磷酶-3(PON3)酶聯(lián)免疫試劑盒（CSB-E15783h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的PON3含量。PON3 是一個重要靶點。它屬于對氧磷酶家族，廣泛存在于高密度脂蛋白表面。研究發(fā)現(xiàn) PON3 具有抗氧化、抗炎等特性，其機制可能與催化水解脂質(zhì)過氧化物、調(diào)節(jié)細胞信號通路等有關(guān)，在心血管疾病等領(lǐng)域有潛在研究價值。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域，可助力探索PON3在心血管疾病、糖尿病、非酒精性脂肪肝等代謝異常相關(guān)疾病中的分子機制，或用于評估藥物干預(yù)對脂質(zhì)過氧化及炎癥反應(yīng)的影響，為體外實驗研究提供可靠的數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    2810004E20 ELISA Kit; AI786302 ELISA Kit; MGC95026 ELISA Kit; Paraoxonase 3 ELISA Kit; Pon3 ELISA Kit; PON3_HUMAN ELISA Kit; Serum paraoxonase/lactonase 3 ELISA Kit
  • 縮寫：
    PON3
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.156 ng/mL-10 ng/mL
  • 靈敏度：
    0.039 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human PON3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %96
    Range %90-106
    1:2000Average %91
    Range %82-99
    1:4000Average %89
    Range %80-97
    1:8000Average %93
    Range %85-102
  • 回收率：
    The recovery of human PON3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9785-105
    EDTA plasma (n=4)9588-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.472 2.411 2.442 2.356
    51.873 1.860 1.867 1.781
    2.51.264 1.243 1.254 1.168
    1.250.721 0.714 0.718 0.632
    0.6250.542 0.568 0.555 0.469
    0.3120.282 0.296 0.289 0.203
    0.1560.206 0.217 0.212 0.126
    00.085 0.086 0.086
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
  • 基因功能參考文獻：
    1. PON3 suppressed cell proliferation in vivo and in vitro, which was attributed to its cell-cycle arrest effect. PMID: 27661119
    2. PON3 protein can be detected in plasma and resides in the high-density lipoprotein fraction and protects against Oxidative stress by hydrolyzing certain oxidized lipids in lipoproteins, macrophages and Atherosclerotic lesions. PMID: 29308836
    3. Although inappropriate promoter methylation was not invariantly associated with reduced transcript expression, a significant association was apparent for the ARHGEF4, PON3, STAT5a, and VAX2 gene transcripts (P<0.05). Herein, we present the first genome-wide DNA methylation analysis in a unique HG-NMIBC cohort, showing extensive and discrete methylation changes relative to normal bladder and low-intermediate-grade tumor PMID: 26929985
    4. Low PON3 expression is associated with hepatocellular carcinoma progression. PMID: 27553024
    5. This study showed that the PI3K/Akt pathway is up-regulated by the expression of PON3 in oral squamous cell carcinoma by AP-1. PMID: 27923688
    6. PON3 SNP rs13226149 was associated with intracerebral hemorrhage in log-additive and dominant models. The A allele of rs13226149 contributed to the decreased risk of ICH. SNP rs1053275 was not associated with ICH. PMID: 26227792
    7. Interaction of PON-3 and PON-1 and HDL are associated with the presence and severity of coronary artery disease in type 2 diabetes mellitus patients. PMID: 25964115
    8. PON3 in HDL may be an important protein in preventing atherosclerosis. PMID: 25723336
    9. PON3 is upregulated in cancer tissues and protects against mitochondrial superoxide-mediated cell death. PMID: 22441669
    10. Our results suggest that low serum paraoxonase activity is a risk factor for Alzheimer disease. Furthermore, multiple variants in PON influence serum paraoxonase activity and their effects may be synergistic. PMID: 20980077
    11. Increased serum paraoxonase-3 concentration is associated with insulin sensitivity in peripheral artery disease and with inflammation in coronary artery disease. PMID: 22153698
    12. This study reports for the first time an important increase in serum PON3 concentrations in HIV-infected patients that is associated with their oxidative status and their treatment with NNRTI. PMID: 22003209
    13. investigation of whether gene silencing of Pon3 causes oxidative stress in cell line: Data suggest that PON3 knockdown reduces cell proliferation and total antioxidant capacity at ambient oxygen levels. PMID: 21952037
    14. Serum PON3 concentrations showed a moderate influence (about 10% variation) by PON3 promoter polymorphisms. PMID: 21335322
    15. Paraoxonase-3, a putative circulating antioxidant, is systemically up-regulated in late gestation in the fetal rat, sheep, and human PMID: 20463093
    16. Decreased coronary heart disease risk associated with polymorphisms of paraoxonase 1. PMID: 12151850
    17. Organophosphates are hydrolyzed almost exclusively by PON1, lovastatin and spironolactone are hydrolyzed only by PON3. All three proteins hydrolyze and thereby inactivate N-acyl-homoserine lactones, which are quorum-sensing signals of pathogenic bacteria. PMID: 15772423
    18. The refolded recombinant PON3 exhibited similar antioxidant activity to that of PON3 purified from the soluble fraction of cell lysate and could effectively protect LDL from Cu2+ induced oxidation. PMID: 16139510
    19. Elevated human paraoxanase 3 protein expression significantly decreases atherosclerotic lesion formation and adiposity in male transgenic mice. PMID: 17379834
    20. The rates of hydrolysis of estrogen esters by the paraoxonases is PON3 > PON1 > PON2; diesters are better substrates for the PONs and are very efficiently hydrolyzed, particularly by PON3. PMID: 17412306
    21. Report protective effects of transgene expressed human PON3 against CCl4-induced subacute liver injury in mice. PMID: 19345057
    22. The directed evolution and characterization of recombinant variants of serum paraoxonase PON3, is described. PMID: 19492856

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  • 亞細胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Paraoxonase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9206

    OMIM: 602720

    KEGG: hsa:5446

    STRING: 9606.ENSP00000265627

    UniGene: Hs.440967



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