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Human Pancreatic secretory trypsin inhibitor(SPINK1) ELISA kit

  • 中文名稱:
    人絲氨酸肽酶抑制劑,Kazal 1型(SPINK1)酶聯免疫試劑盒
  • 貨號:
    CSB-EL022575HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人絲氨酸肽酶抑制劑,Kazal 1型(SPINK1)酶聯免疫試劑盒(CSB-EL022575HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SPINK1含量。SPINK1是重要的研究靶點。它是一種絲氨酸蛋白酶抑制劑,在多種生理過程中發揮作用。研究發現其在胰腺癌等癌癥中異常表達,可能參與腫瘤細胞的增殖、侵襲和轉移,其調控機制等正成為科研重點,以探索新的治療方向。試劑盒檢測范圍為78 pg/ml- 5000 pg/ml,適用于基礎科研中探索SPINK1在胰腺疾病、消化道腫瘤等病理過程中的分子機制,或用于藥物開發模型中評估化合物對SPINK1表達水平的調控作用,也可作為生物標志物研究的工具,為體外實驗提供定量數據支持本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    ISK1_HUMAN ELISA Kit; Pancreatic secretory trypsin inhibitor ELISA Kit; Pancreatic serine protease inhibitor Kazal ELISA Kit; PCTT ELISA Kit; PSTI ELISA Kit; Serine protease inhibitor Kazal type 1 ELISA Kit; Serine protease inhibitor Kazal-type 1 ELISA Kit; Spink 3 ELISA Kit; SPINK1 ELISA Kit; Spink3 ELISA Kit; TaTI ELISA Kit; TCP ELISA Kit; Tumor associated trypsin inhibitor ELISA Kit; Tumor-associated trypsin inhibitor ELISA Kit
  • 縮寫:
    SPINK1
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 pg/ml - 5000 pg/ml
  • 靈敏度:
    19.5 pg/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cell Biology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human SPINK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:2

    Average %

    95

    Range %

    90-100

    1:4

    Average %

    90

    Range %

    83-97

    1:8

    Average %

    89

    Range %

    84-95

    1:16

    Average %

    92

    Range %

    88-97

  • 回收率:

    The recovery of human SPINK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    89-98

    EDTA plasma (n=4)

    99

    95-105

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.415

    2.534

    2.475

    2.333

    2500

    1.876

    1.967

    1.922

    1.780

    1250

    1.405

    1.428

    1.417

    1.275

    625

    0.927

    0.929

    0.928

    0.786

    312

    0.491

    0.501

    0.496

    0.354

    156

    0.352

    0.343

    0.348

    0.206

    78

    0.247

    0.234

    0.241

    0.099

    0

    0.139

    0.145

    0.142

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Serine protease inhibitor which exhibits anti-trypsin activity. In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.; In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
  • 基因功能參考文獻:
    1. 3 siblings with near identical clinical manifestations of early onset acute recurrent pancreatitis (ARP), all possessing the same heterozygous for serine protease inhibitor, Kazal type 1 (SPINK1) mutation c.55+1G>A. PMID: 29346218
    2. We demonstrate for the first time a significant decrease of SPINK1 levels after surgical decompression of pancreatic duct and a reduction of trypsin activity analysis after endoscopic decompression PMID: 29525377
    3. SPINK1 over expression is associated with prostate cancer specific mortality in at risk men with biochemical and clinical recurrence after prostatectomy PMID: 27238617
    4. Data show that alteration in beta-catenin expression, a core component of the CDH17/beta-catenin axis, in tumors affects serine peptidase inhibitor Kazal type 1 (SPINK1) serum levels in hepatocellular carcinoma (HCC) patients. PMID: 28631187
    5. Findings indicate that the serine protease inhibitor, Kazal type 1 (SPINK1) p.N34S allele may cause reduced SPINK1 expression. PMID: 28609377
    6. Having correlated our findings with current knowledge of SPINK1's role in exocrine pancreas pathophysiology, we propose that complete and partial functional losses of the SPINK1 gene are associated with quite distinct phenotypes, the former causing a new pediatric disease entity of severe infantile isolated exocrine pancreatic insufficiency (EPI) . PMID: 28945313
    7. results suggest that rs142703147:C>A, which disrupts a PTF1L-binding site within an evolutionarily conserved HNF1A-PTF1L cis-regulatory module located upstream of the SPINK1 promoter, contributes to the chronic pancreatitis risk haplotype. PMID: 28556356
    8. Studied mutations in cationic trypsinogen (PRSS1) and serine protease inhibitor Kazal type 1 (SPINK1) and their association with alcoholic chronic pancreatitis (ACP) and idiopathic chronic pancreatitis. PMID: 29641165
    9. Studies indicate that serine peptidase inhibitor Kazal type 1 (SPINK1) gene, particularly the N34S mutation, has a genetic association with the development of pancreatitis [Meta-analysis]. PMID: 28984793
    10. association between SPINK1 p.N34S gene variation and chronic pancreatitis [review, meta-analysis] PMID: 28546062
    11. We present the case of a child with a homozygous mutation N34S in the SPINK1 gene, leading to acute recurrent pancreatitis and ultimately to chronic pancreatitis PMID: 28229614
    12. Used in silico splicing prediction programs to prioritize SPINK1 intronic variants for further quantitative RT-PCR analysis the non-pathological c.194 + 13T > G variant was predicted by different programs to generate a new & viable donor splice site, the prediction scores being comparable to those for the physiological c.194 + 2T donor splice site & even higher than those for the physiological c.87 + 1G donor splice site. PMID: 28472998
    13. Gene mutations were present in PRSS1 in 26 patients with acute recurrent and chronic pancreatitis, SPINK1 in 23, CTRC in 3, and CPA1 in 5. In the 31 patients with mutations in SPINK1, CTRC, or CPA1, 16 (51.6%) had homozygous or heterozygous mutations with other mutations. PMID: 27409067
    14. SPINK1 can associate with EGFR to promote the expression of cell proliferation-related and anti-apoptosis-related genes/proteins; inhibit the expression of pro-apoptosis-related genes/proteins via p38, ERK, and JNK pathways; and consequently promote the proliferation of BRL-3A cells. PMID: 28845526
    15. The present study demonstrates for the first time that both fibroblast-derived and recombinant IL-6 induces SPINK1 expression and secretion in in colorectal adenocarcinoma. PMID: 26663388
    16. Absolute exclusivity between SPINK1 overexpression and homozygous PTEN deletion in localized PCA. PMID: 27738792
    17. It is the first time to simultaneously detect SPINK1 and ERG expression in initially diagnosed bone metastatic prostate cancer. The over-expression of SPINK1 was not only related to poor PSA response, but also significantly associated with the occurrence of castration-resistant prostate cancer, especially in those with much more aggressive phenotype PMID: 27159572
    18. We describe a case of malignant pancreatic cancer diagnosed in a young patient with chronic pancreatitis who is a SPINK 1 heterozygote gene mutation carrier PMID: 27358244
    19. Mutations in CFTR, SPINK1 or PRSS1 are present in one third of pediatric acute recurrent (ARP) or chronic (CP) pancreatitis with no other cause. PMID: 26692446
    20. TATI proved to be a sensitive indicator of disease recurrence and distant metastasis, with a sensitivity of 84.4% and 75.7%, respectively. PMID: 26350184
    21. Sixteen patients exhibited heterozygous pathogenic variants of SPINK1, including c.194+2T>C (n=12), p.N34S (n=3), and a novel pathogenic splicing variation c.194+1G>A. PMID: 27578509
    22. Data indicate that SPINK1 expression status shows a lower rate of concordance between primary prostate cancer (PCA) site and nodule metastases, than those of ERG and PTEN. PMID: 26725250
    23. Approx 25% of discontinuously involved prostate biopsy cores showed tumor foci with discordant ERG/SPINK1 status, consistent with multiclonal disease. PMID: 26743468
    24. Letter/Case Report: high penetrance of the PRSS1 A16V mutation caused by SPINK1 N34S and CFTR TG11-5T co-mutations associated with pancreatitis. PMID: 26658045
    25. Identify SPINK1 promoter variants exhibiting significant functional defects that should be considered potential risk factors for chronic pancreatitis. PMID: 26348468
    26. expression represents an independent prognostic factor for poor survival in ovarian carcinoma patients PMID: 26437224
    27. The SPINK1 protein seems to play a role in tumor proliferation. PMID: 26037168
    28. neither ERG nor SPINK1 appear to be useful biomarkers for prognostication of early stage prostate cancer PMID: 26172920
    29. The majority of children with genetic predisposition to idiopathic acute and acute recurrent pancreatitis have the SPINK1 mutation. PMID: 25981744
    30. Patients with SPINK1 N34S mutations were more likely to develop a Dilated duct, Calcifications, and Diabetes as Long-term Outcome of Endoscopic Treatments in Idiopathic Chronic Pancreatitis. PMID: 26632706
    31. Evidence suggests that SPINK1 is an important growth factor that connects chronic inflammation and cancer. PMID: 25804623
    32. Over-expression of FOXP1 and SPINK1 may participate in the carcinogenesis of hepatitis B virus related cirrhosis. PMID: 26054682
    33. SPINK1 plays a role as a growth factor, signaling through the EGFR pathway in pancreatic ductal adenocarcinoma and neoplasms, and that the EGFR is involved in the malignant transformation of IPMN. PMID: 23475261
    34. In Israel, pediatric as well as adult recurrent acute pancreatitis and chronic pancreatitis are often associated with cationic trypsinogen, SPINK1, chymotrypsin C, or Cystic Fibrosis Transmembrane Conductance Regulator mutations. PMID: 25383785
    35. Mutation p.N34S in SPINK1 may predispose patients to acute pancreatitis, especially in those abusing alcohol, and may promote a more severe course of the disease. PMID: 26100556
    36. This study shows that PRSS1, SPINK1 and CFTR mutations do not play a role in alcoholic and idiopathic chronic pancreatitis patients. PMID: 25835118
    37. no mutation associated with pancreatitis PMID: 26376395
    38. OPN, SPINK1, GPC3 and KNPA2 were significantly over-expressed in HCC tissues. These genes may be useful in developing future biomarkers and therapeutic strategies for HCC PMID: 25862856
    39. SPINK1 promoter variants are likely to be associated with the risk of BPH. PMID: 26124326
    40. These data highlight the importance of SPINK1/TATI as a tumor marker for HCV-induced HCC and may lead to a better understanding of HCV-induced hepatocarcinogenesis. PMID: 25531719
    41. Our results suggest that SPINK1 protein expression may not be a predictor of recurrence or lethal prostate cancer amongst men treated by radical prostatectomy PMID: 24687926
    42. One novel SPINK1 mutation was identified in Mexican patients with early onset idiopathic recurrent acute pancreatitis. PMID: 25206283
    43. Results suggest that sequence evaluation of the SPINK1 promoter region in patients with chronic pancreatitis is justified as part of the etiological investigation. PMID: 25792561
    44. ERG and TFF3 expression characterize 2 distinct subsets of prostate cancer, with a more aggressive subgroup of TFF3-expressing tumors that express SPINK1. PMID: 25639219
    45. The p.N34S mutation in SPINK1 gene was found more frequently in patients with AP in the Indian population, irrespective of disease etiology and whether the disease was recurrent or not, and with earlier age of onset. PMID: 24844923
    46. The SPINK1 mutation frequency was significantly decreased in patients with pancreatic cancer in comparison with patients with idiopathic pancreatitis but varied not significantly in comparison with healthy controls. PMID: 25003218
    47. study found a significant association of SPINK1 (N34S) gene polymorphism with chronic pancreatitis PMID: 24616641
    48. An association between strong claudin-5 expression and higher serum levels of TATI (p=0.04) and CA125 (p=0.008) were found PMID: 25667483
    49. Over-expression of TATI predicts an unfavorable outcome in several cancers and serum TATI can be used to identify patients at increased risk of aggressive disease--{REVIEW} PMID: 24583226
    50. Data indicate epidermal growth factor receptor (EGFR)+/tumor-associated trypsinogen inhibitor (TATI)+ patients showed better survival. PMID: 24204699

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  • 相關疾病:
    Pancreatitis, hereditary (PCTT); Tropical calcific pancreatitis (TCP)
  • 亞細胞定位:
    Secreted.
  • 數據庫鏈接:

    HGNC: 11244

    OMIM: 167790

    KEGG: hsa:6690

    STRING: 9606.ENSP00000296695

    UniGene: Hs.407856



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