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Human Pancreatic Amylase,PAMY ELISA Kit

  • 中文名稱:
    人胰淀粉酶(PAMY)酶聯免疫試劑盒
  • 貨號:
    CSB-E09691h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人胰淀粉酶(PAMY)酶聯免疫試劑盒(CSB-E09691h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的AMY2A含量。AMY2A是一種編碼α-淀粉酶的基因,主要在胰腺中表達。它通過水解寡糖和多糖中的1,4-α-葡萄糖苷鍵,催化淀粉和糖原的消化。研究顯示,AMY2A與胰島素抵抗和糖尿病有關,其表達水平與血糖水平相關。近期研究揭示了AMY2A在淀粉消化和血糖調節中的重要作用,為糖尿病治療提供了新的靶點。試劑盒檢測范圍為47 mU/mL-3000 mU/mL,該產品適用于科研場景中胰腺外分泌功能評估、消化系統疾病機制研究或藥物干預效果分析,例如在實驗動物模型或體外細胞模型中追蹤PAMY表達動態變化。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    AMY2A ELISA Kit; Pancreatic alpha-amylase ELISA Kit; PA ELISA Kit; EC 3.2.1.1 ELISA Kit; 1,4-alpha-D-glucan glucanohydrolase ELISA Kit
  • 縮寫:
    AMY2A
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    47 mU/mL-3000 mU/mL
  • 靈敏度:
    11.75 mU/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PAMY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 89-99    
    1:2 Average % 102    
    Range % 96-107    
    1:4 Average % 94    
    Range % 87-98    
    1:8 Average % 86    
    Range % 81-92    
  • 回收率:
    The recovery of human PAMY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 98 91-103    
    EDTA plasma (n=4) 102 96-105    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    mU/ml OD1 OD2 Average Corrected    
    3000 2.066 2.154 2.110 1.992    
    1500 1.496 1.589 1.543 1.425    
    750 1.007 1.043 1.025 0.907    
    375 0.634 0.647 0.641 0.523    
    187.5 0.375 0.382 0.379 0.261    
    93.75 0.267 0.273 0.270 0.152    
    46.88 0.186 0.189 0.188 0.070    
    0 0.117 0.118 0.118      
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 基因功能參考文獻:
    1. Data show that serum amylase was found to be correlated with waist circumference, ghrelin and PYY3-36. PMID: 26495778
    2. Data show that amylase-alpha2A autoantibodies may help to diagnosis of autoimmune pancreatitis (AIP) and to differentiate AIP subtypes. PMID: 26335011
    3. Studies indicate that chloride activates alpha-amylases and ACE, and gaining insight into the potential mechanisms by which chloride functions in PSII. PMID: 21366335
    4. Mammalian pancreatic alpha-amylases share a common carbohydrate binding activity and specifically bind to the intestinal brush border. PMID: 22584580
    5. We demonstrated that AMY2A is frequently silenced in gastric carcinoma deletion 1p21.1. PMID: 20428766
    6. A new approach for the discovery and subsequent structural elucidation of oligosaccharide-based inhibitors of alpha-amylases based upon autoglucosylation of known alpha-glucosidase inhibitors is presented. PMID: 15304511
    7. Autoantibody against amylase alpha-2A is a novel diagnostic marker for both autoimmune pancreatitis and fulminant type 1 diabetes. PMID: 19001184

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  • 亞細胞定位:
    Secreted, extracellular space.
  • 蛋白家族:
    Glycosyl hydrolase 13 family
  • 組織特異性:
    Detected in pancreas (at protein level).
  • 數據庫鏈接:

    HGNC: 477

    OMIM: 104650

    KEGG: hsa:279

    STRING: 9606.ENSP00000397582

    UniGene: Hs.654437



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