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Human Osteocalcin/Bone gla protein,OT/BGP ELISA kit

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產品描述：

人骨鈣素/骨谷氨酸蛋白(OT/BGP)酶聯免疫試劑盒（CSB-E05128h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BGLAP含量。BGLAP（骨鈣素）是一種由成骨細胞分泌的激素，參與骨骼重塑和能量代謝。其羧化形式負向調節骨形成，同時促進骨吸收和礦化，對磷灰石和鈣有強親和力。研究顯示，BGLAP在骨骼發育、成骨細胞分化和生物礦化組織中發揮重要作用。試劑盒檢測范圍為31.25 pg/ml-2000 pg/ml，靈敏度為7.8 pg/ml。適用于骨代謝相關基礎研究，如骨質疏松癥動物模型評估、骨骼發育機制探索、骨組織工程材料生物活性分析等科研領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。

別名：

BGLAP ELISA Kit; BGP ELISA Kit; Bone gamma carboxyglutamate (gla) protein ELISA Kit; Bone gamma carboxyglutamate gla protein osteocalcin ELISA Kit; Bone gamma carboxyglutamate protein ELISA Kit; Bone Gla protein ELISA Kit; Gamma carboxyglutamic acid containing protein ELISA Kit; Gamma-carboxyglutamic acid-containing protein ELISA Kit; OC ELISA Kit; OCN ELISA Kit; OSTCN_HUMAN ELISA Kit; Osteocalcin ELISA Kit; OTTHUMP00000016586 ELISA Kit; PMF1 ELISA Kit

縮寫：

BGLAP

Uniprot No.：

P02818

種屬：

Homo sapiens (Human)

樣本類型：

serum, plasma, tissue homogenates

檢測范圍：

31.25 pg/ml-2000 pg/ml

靈敏度：

7.8 pg/mL

反應時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領域：

Metabolism

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-106
1:2	Average %	99
	Range %	93-105
1:4	Average %	94
	Range %	86-99
1:8	Average %	97
	Range %	91-105

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

2000

2.552

2.634

2.593

2.483

1000

1.764

1.667

1.716

1.605

500

1.242

1.228

1.235

1.125

250

0.747

0.729

0.738

0.628

125

0.476

0.451

0.463

0.353

62.5

0.324

0.333

0.329

0.218

31.25

0.194

0.184

0.189

0.079

0

0.111

0.109

0.110

本試劑盒所含材料：

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human BGLAP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled BGLAP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數據處理：

ELISA操作視頻及標曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.