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  4. Human Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit

Human Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit

  • 中文名稱：
    人鳥氨酸氨甲酰基轉移酶,線粒體(OTC)酶聯免疫試劑盒
  • 貨號：
    CSB-EL017270HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人鳥氨酸氨甲酰基轉移酶,線粒體(OTC)酶聯免疫試劑盒（CSB-EL017270HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的OTC含量。試劑盒檢測范圍為1.25 mU/mL-80 mU/mL，適用于科研領域中對肝代謝疾病模型、遺傳性尿素循環障礙機制、藥物肝毒性評估以及細胞能量代謝相關研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    EC 2.1.3.3 ELISA Kit; MGC129967 ELISA Kit; MGC129968 ELISA Kit; MGC138856 ELISA Kit; OCTD ELISA Kit; Ornithine Carbamoyltransferase ELISA Kit; Ornithine carbamoyltransferase mitochondrial ELISA Kit; Ornithine carbamoyltransferase, mitochondrial ELISA Kit; Ornithine transcarbamylase ELISA Kit; Otc ELISA Kit; OTC_HUMAN ELISA Kit; OTCase ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    1.25 mU/mL-80 mU/mL
  • 靈敏度：
    0.312 mU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human OTC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-89
    1:2 Average % 98
    Range % 94-103
    1:4 Average % 85
    Range % 81-89
    1:8 Average % 99
    Range % 94-104
  • 回收率：
    The recovery of human OTC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 100 95-105
    EDTA plasma (n=4) 86 83-89
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected
    80 1.964 1.900 1.932 1.748
    40 1.764 1.789 1.777 1.593
    20 1.579 1.502 1.541 1.357
    10 1.257 1.201 1.229 1.045
    5 0.901 0.931 0.916 0.732
    2.5 0.591 0.587 0.589 0.405
    1.25 0.362 0.371 0.367 0.183
    0 0.188 0.180 0.184 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.
  • 基因功能參考文獻：
    1. Whole exome sequencing successfully identified disease-causing mutations including two novel ones: the c.209_210delAA (p.Lys70Argfs*17) and the c.850T>A (p.Tyr284Asn). PMID: 30223008
    2. indicate that the proportion of about 20-30% of hepatocytes expressing the functional OTC protein is not sufficient to maintain metabolic stability. X-inactivation ratios assessed in liver biopsies taken from heterozygous females with X-linked disorders should not be considered representative of the whole liver PMID: 29623395
    3. The measurement of serum ornithine carbamoyltransferase concentration may provide a useful marker of disease severity, and thus could be a useful marker for a high risk of hepatocellular carcinoma occurrence. PMID: 28824294
    4. Low expression of OTC is associated with glioblastoma. PMID: 27431689
    5. In Korean patients with OTC deficiency, mutations in OTC are genetically heterogeneous. PMID: 25994866
    6. Sanger sequencing of the ornithine transcarbamylase (OTC) gene revealed a novel hemizygous deletion at the fourth nucleotide of intron 4 (c.386+4delT) in a child with hyperammonemia and his asymptomatic mother. PMID: 26446336
    7. Ornithine transcarbamylase deficiency was genetically heterogeneous in seven Korean patients with confirmed ornithine transcarbamylase deficiency diagnosis by biochemical findings and/or genetic analysis, together with two novel mutations in the OTC gene PMID: 25011434
    8. The corresponding OTC tissue enzyme activities were between 3-6% of normal control in mouse and human liver. The use of the cryptic splice sites was reproduced in minigenes carrying murine or human mutant sequences PMID: 25853564
    9. OTC mutation and phenotype in ornithine transcarbamylase deficiency PMID: 26059767
    10. aim of this study was to provide clues for recognition of OTCD in adults and analyze the environmental factors that, interacting with OTC gene mutations, might have triggered acute clinical manifestations PMID: 25026867
    11. HNF-4alpha most likely plays an essential role in the initiation of OTC transcription in human. PMID: 25056436
    12. Data indicate that all of the three patients have carried ornithine transcarbamylase gene mutations, patients 1 and 2 were both hemizygous for mutation c.586G> A(p.D196N). PMID: 25297582
    13. V339G and W332S mutations of OTC have been discovered for the first time PMID: 24711021
    14. carriers of the ornithine transcarbamylase (OTC) mutation are at risk for developing hyperammonemia coma during the postpartum period and at times of metabolic stress. PMID: 21956151
    15. In patients with a clinical and biochemical presentation of OTCD and negative OTC sequencing, whole genome or targeted chromosomal microarray analysis (CMA) with coverage of the OTC and neighboring genes should be performed as a reflex test. PMID: 20817516
    16. Mutations in the regulatory regions of OTC can lead to ornithine carbamoyltransferase deficiency and should be included in genetic testing. PMID: 20127982
    17. These results indicate that mutant alleles with late-onset OCT deficiency syndrome have recurrently arisen, have been retained in some populations, and some appear to hvae a common ancestor. PMID: 19893582
    18. deficiency in ornithine carbamoyltransferase enzymatic function conferred by the R40H mutation is likely caused by enhanced degradation of the preprotein in the cytosol. PMID: 11768581
    19. ornithine transcarbamylase (OTC) deficiency: review of mutations and polymorphisms in the human ornithine transcarbamylase gene PMID: 11793468
    20. seven novel missense mutations, two splice-site mutations, two microdeletions and a polymorphic amino acid substitution in the gene for ornithine transcarbamylase (OTC) in patients with OTC deficiency PMID: 11793483
    21. novel mutations in the ornithine transcarbamylase gene PMID: 12083811
    22. Haplotype analysis and phylogeny of polymorphisms of this protein were studied in two male populations. PMID: 12516615
    23. Using 2 (CA)n flanking markers of the OTC gene (DXS997 & DXS1068), the haplotypic background of 37 different mutational events was defined & compared with random control chromosomes. 1 particular haplotype is a risk factor for carrying OTC mutations. PMID: 15300856
    24. Mutations in the human ornithine transcarbamylase (OTC) gene is detected in patients with Hyperammonemia-induced encephalopathy due to ornithine transcarbamylase deficiency. PMID: 15692798
    25. Novel mutations within the OTC gene are associated with Ornithine transcarbamylase (OTC) deficiency disorder. PMID: 16786505
    26. Novel mutations p.Leu9X, p.Arg26Pro, p.Gly100Arg, p.Met205Thr, p.Lys221Asn, p.Asp249Gly, p.Phe281Ser, p.Val323Met, c.571delC, c.853delC, and c.796-805del are associated with OTC deficiency. PMID: 17041896
    27. Describe a contiguous gene syndrome involving the RPGR, OTC and TM4SF2 genes in a male patient with severe neonatal ornithine transcarbamylase deficiency. PMID: 17570074
    28. Data show that OCT/ALT is a potent indicator for the diagnosis and the prognosis of hepatocellular carcinoma. PMID: 17570354
    29. our results suggest the involvement of a new pathway in AD brains involving the urea cycle. PMID: 17893704
    30. In ten families with late-onset ornithine transcarbamylase (OTC) deficiency in male patients, three mutant alleles-R40H, R277W, and Y55D-were identified. PMID: 18030415
    31. Two known and three novel mutations of the ornithine transcarbamylase (OTC) gene are reported in five Japanese patients with OTC deficieny including two neonatal-onset, one late-onset, and two symptomatic female patients. PMID: 18204299
    32. The rs5963409 minor allele was weakly but significantly associated with an increased risk of developing AD. PMID: 18983895
    33. Deletions and gene rearrangements of OTC gene are associated with Ornithine Carbamoyltransferase Deficiency Disease. PMID: 19138872
    34. Hypocitrullinemia in expanded newborn screening is not a reliable marker for OTCD. PMID: 19359120
    35. the identification of novel disease-causing mutations in Ornithine transcarbamylase deficiency and increases the knowledge on possible mutational mechanisms generating deletions in ornithine transcaramylase. PMID: 19475717
    36. OTC rs5963409 polymorphism may be associated with hypertension and coronary vasomotion in males PMID: 19574962

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  • 相關疾病：
    Ornithine carbamoyltransferase deficiency (OTCD)
  • 亞細胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    ATCase/OTCase family
  • 組織特異性：
    Mainly expressed in liver and intestinal mucosa.
  • 數據庫鏈接：

    HGNC: 8512

    OMIM: 300461

    KEGG: hsa:5009

    STRING: 9606.ENSP00000039007

    UniGene: Hs.117050



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