Human Orexin B,OX-B ELISA Kit
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中文名稱:人食欲素/阿立新B(OX-B)酶聯免疫試劑盒
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貨號:CSB-E09863h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人食欲素/阿立新B(OX-B)酶聯免疫試劑盒(CSB-E09863h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的OX-B含量。人食欲素/阿立新B(OX-B)是一種由下丘腦神經元分泌的神經肽,能促進食欲、調節睡眠和覺醒。研究發現,OX-B與食欲素受體結合后,可調節攝食行為、睡眠周期及情緒等生理過程。針對OX-B及其受體的研究,為治療肥胖、失眠等疾病提供了新的治療靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,該產品適用于神經生物學研究中食欲素系統的功能解析、代謝調節機制探索,或睡眠障礙動物模型中的生物標志物分析,為中樞神經系統調控研究提供可靠的檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:OX-B
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:62.5 pg/mL-4000 pg/mL
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靈敏度:15.6 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Metabolism
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays):CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human OX-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 107 Range % 95-111 1:2 Average % 100 Range % 93-104 1:4 Average % 94 Range % 86-97 1:8 Average % 100 Range % 92-104 -
回收率:
The recovery of human OX-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 84-95 EDTA plasma (n=4) 108 97-112 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 4000 2.312 2.378 2.345 2.212 2000 1.386 1.346 1.366 1.233 1000 0.820 0.812 0.816 0.683 500 0.523 0.504 0.514 0.381 250 0.370 0.356 0.363 0.230 125 0.278 0.268 0.273 0.140 62.5 0.209 0.203 0.206 0.073 0 0.132 0.134 0.133 -
數據處理:
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貨期:3-5 working days
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