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中文名稱:人阿立新A(Orexin A)酶聯免疫試劑盒
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貨號:CSB-E08859h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人阿立新A(Orexin A)酶聯免疫試劑盒(CSB-E08859h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的Orexin A含量。Orexin A,又稱食欲素A,是一種由下丘腦神經元分泌的神經肽,主要作用是調節覺醒、睡眠和食欲。其通過激活OX1R和OX2R受體,影響神經遞質系統,如GABA和谷氨酸,從而調節睡眠-覺醒周期和能量代謝。研究顯示,Orexin A在治療睡眠障礙和調節食欲方面具有潛在應用價值。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于基礎科研中探索Orexin A在神經系統功能、代謝調控機制以及相關疾病動物模型中的作用,例如通過檢測不同生理狀態或藥物干預下生物樣本中Orexin A的動態變化,為神經生物學、內分泌學及行為學研究提供定量分析工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:Hcrt ELISA Kit; Hcrt1 ELISA Kit; Hcrt2 ELISA Kit; Hypocretin ELISA Kit; Hypocretin neuropeptide precursor ELISA Kit; Hypocretin-1 ELISA Kit; Hypocretin-2 ELISA Kit; OREX_HUMAN ELISA Kit; Orexin ELISA Kit; Orexin precursor ELISA Kit; Orexin-B ELISA Kit; OX ELISA Kit; PPOX ELISA Kit
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縮寫:Orexin A
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Uniprot No.:
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.156 ng/mL-10 ng/mL
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靈敏度:0.039 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Metabolism
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 84 Range % 80-92 1:2 Average % 92 Range % 87-96 1:4 Average % 84 Range % 81-86 1:8 Average % 102 Range % 100-106 -
回收率:
The recovery of human orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 87-96 EDTA plasma (n=4) 97 95-105 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.334 2.556 2.445 2.341 5 2.161 2.175 2.168 2.064 2.5 1.645 1.756 1.701 1.597 1.25 1.257 1.267 1.262 1.158 0.625 0.818 0.886 0.852 0.748 0.312 0.547 0.568 0.558 0.454 0.156 0.331 0.356 0.344 0.240 0 0.102 0.106 0.104 -
數據處理:
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貨期:3-5 working days
引用文獻
- Effect of two training on orexinAand lipid profile in obese adolescent boys Fezzeh MOHAMADHASAN.et al,Bulletin de la Socit Royale des Sciences de Lige,2016
- The effects of endurance training and high intensity interval training on orexin-A and anthropometric parameters in obese adolescent boys Mohammadhassani F.et al,Journal of School of Public Health and Institute of Public Health Research,2015
相關產品
靶點詳情
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功能:Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
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基因功能參考文獻:
- Like OX1R, OX2R has an N-terminal helix important for binding the orexin peptide. PMID: 29225076
- this study, both Perry syndrome and MSA had significantly reduced orexin immunoreactivity in the nucleus basalis of Meynert. PMID: 28651750
- Orexin A levels correlated with age in females and were significantly elevated in PWS even after these effects were controlled. These findings support the hypothesis that dysregulation of orexin signaling may contribute to behavioral problems and hyperphagia in PWS. PMID: 27214028
- Orexin levels do not play a role in the control of nocturnal blood pressure dipping in patients with narcolepsy type 1. PMID: 29023559
- No association was observed between rs9902709 in HCRT and OSAHS (obstructive sleep apnea) risk in Chinese Han population. PMID: 27509181
- findings show that in few patients with narcolepsy with cataplexy, symptoms and CSF hypocretin-1 and histamine levels can change over time PMID: 28364477
- The plasma orexin-A level was associated with physical activity in obese and overweight people, including many aspects of daily life, such as working, domestic work, and walking especially. PMID: 27038345
- Orexin-A is associated with increased phosphorylated P-Tau in normal elderly individuals. Increases in orexin-A and P-Tau might be a consequence of the reduction in the proportion of the deeper, more restorative slow wave sleep and rapid eye movement sleep reported with aging. PMID: 26951396
- actigraphy gives additional information about the stabilization of sleep in patients with narcolepsy type 1; very low hypocretin levels associate with more wake intruding into sleep PMID: 26856902
- Exercise induced a significant increase in plasmatic orexin A with a peak at 3o minutes. PMID: 27665420
- There was also a negative correlation between Epworth sleepiness scale scores and orexin levels in Prader-Willi syndrome patients. Decreased cerebrospinal fluid orexin levels in Prader-Willi syndrome may play an important role in severity of obesity and excessive daytime sleepiness. PMID: 26738920
- orexin system may be involved even in the earliest stages of AD, resulting in prolonged sleep latency, reduced sleep efficiency, and REM sleep impairment. PMID: 26973111
- Rapid-onset obesity with hypothalamic dysfunction, hypoventilation, and autonomic dysregulation is highly unlikely to be caused by mutations in the exons of the gene for hypocretin. PMID: 26555080
- Data indicate a positive correlation of nesfatin-1 and a negative correlation of orexin-A with body mass index. PMID: 26831553
- This study showed that orexin A induced autophagy through the ERK pathway in HCT116 human colon cancer cells. PMID: 26572581
- Low circulating Orexin A was found to be significantly associated with hyperlipidemia, obesity and obesity-related disorders in North Indian premenopausal women. PMID: 27530010
- This is the first time a seasonal variation of hcrt-1 levels has been shown, demonstrating that the hcrt system is, like other neurotransmitter systems, subjected to long term modulation PMID: 27008404
- Significantly reduced csf levels of hypocretin-1 were found in cluster headache patients compared with controls. PMID: 25492975
- Study shows Ox immunoreactivity is decreased in the hypothalamus and pontine nuclei of infants who died from sudden infant death syndrome PMID: 25953524
- Results from homology and docking modeling show two alternative binding modes for orexin-A into orexin 1 receptor in concordance with what is known of G protein coupled receptors activation in general and the mutational data. PMID: 25957175
- Patients with schizophrenia had a significantly higher plasma HCRT level than healthy controls. Patients in the high-orexin subgroup had significantly fewer negative and disorganized symptoms, and tended to have fewer perseverative errors. PMID: 25560205
- Data suggest that interaction between the nociceptin/nociceptin receptor system and the orexin/orexin receptor system in neurons of the hypothalamus positively and negatively modulate complex and integrated responses to stress. [REVIEW] PMID: 25677777
- Decrease in Ox expression with normal human maturation and aging. This may contribute to changes in sleep regulation during development and with aging. PMID: 25212464
- Orexin A upregulates the protein expression of OX1R and enhances the proliferation of SGC-7901 gastric cancer cells through the ERK signaling pathway. PMID: 25515760
- Orexin receptor binding was strongly dependent on the extracellular Ca(2+) concentration. PMID: 25132134
- autoimmune encephalitis and neuromyelitis optica patients had significantly lower serum orexin-A levels than both healthy controls and narcolepsy patients PMID: 25218417
- No significant differences were found in the distribution of either genotypic or allelic frequencies between Alzheimer's disease cases and controls in the HCRT polymorphisms. PMID: 24969517
- Hypocretin, MOG, or DNMT1 mutations are exceptional findings in DQB1*06:02 negative narcolepsy cases with hypocretin deficiency PMID: 25197808
- In patients with narcolepsy with cataplexy, deficient CSF HCRT levels were correlated with lower muscle sympathetic nerve activity and heart rate. PMID: 25098533
- the first report clarifying the substantial loss of Hcrt neurons in the LHT and of Hcrt axonal terminals in the LC in DLB and the correlation between the severity of Hcrt neuronal loss and progression of neurofibrillary pathology. PMID: 24704327
- Rapid postoperative increases in plasma Orexin are associated with better improvement of glucose and lipid profiles following bariatric surgery. PMID: 24400115
- lumbar ORX-A levels are significantly higher than ventricular levels in idiopathic normal pressure hydrocephalus patients. PMID: 24172541
- OX1 is upregulated in the transgenic mouse suprachiasmatic nucleus at dusk. PMID: 24599460
- The orexin-A levels were significantly lower in patients with frontotemporal dementia compared to controls. PMID: 23813609
- Sequencing of a large number of early-onset narcolepsy subjects revealed three novel nonsynonymous substitutions within the preprohypocretin protein, two of which were only found in patients with early-onset narcolepsy. PMID: 23643651
- It regulates energy and glucose metabolism.(review) PMID: 24334931
- serum and cerebrospinal fluid orexin-A concentrations decrease after cerebral ischaemia and may play a role in the development of brain injury. PMID: 23569032
- Cerebrospinal fluid (CSF) levels were measured in 33 patients with Alzheimer's disease (AD) and 33 healthy subjects, to investigate the role of MCH and HCRT-1 in AD. PMID: 23667582
- Maternal serum OXA levels decrease, and fetal serum OXA levels increase in women with gestational diabetes. PMID: 22889404
- A sensor platform capable of rapid biomarker detection specific for detecting neuropeptide orexin A, is found in blood and saliva as an indicator of fatigue and cognitive performance, and can be easily expanded. PMID: 23509980
- Our results indicate that serum OXA levels decrease in the serum of women with PCOS. PMID: 23350701
- Serum S100B and orexin A levels may be helpful in differentiating parasomnic pediatric patients with and without epilepsy. PMID: 23406753
- melatonin and hypocretin, may affect Alzheimer's disease pathophysiology, as this may provide a role for lack of sleep and circadian rhythm deterioratio--REVIEW PMID: 22575905
- the changes in the levels of two hypothalamic neuropeptides, hypocretin-1 and melanin-concentrating hormone, measured in the human amygdala, are reported. PMID: 23462990
- Substitution of the deficient neuropeptides by hypocretin agonists may become the causal treatment strategy for narcolepsy--{REVIEW} PMID: 22696207
- [review] The role of neuropeptide orexins has been investigated in many functions mediated by the central nervous system, including sleep and wakefulness, appetite/metabolism, stress response, reward/addiction, and analgesia. PMID: 23034387
- There was a statistically significant difference in orexin expression between nicotine dependent smokers, non-smokers, and cannabis dependent subjects. Promoter methylation did not differ between groups. PMID: 22846875
- We conclude that the hypocretin system is affected in advanced AD PMID: 21546124
- Lower levels of CSF orexin are specific to dementia with Lewy body (DLB) versus Alzheimer's disease (AD) and appear unrelated to Abeta1-42 and alpha-synuclein levels in AD and DLB. PMID: 22207004
- a functional locus within the HCRT gene, which may act to increase HCRT expression and lead to a protective effect against the development of OSAS. PMID: 22302634
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相關疾病:Narcolepsy 1 (NRCLP1)
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亞細胞定位:Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse.
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蛋白家族:Orexin family
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組織特異性:Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
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