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Human Nucleolin(NCL) ELISA kit

  • 中文名稱:
    人核仁蛋白(NCL)酶聯免疫試劑盒
  • 貨號:
    CSB-EL015535HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人核仁蛋白(NCL)酶聯免疫試劑盒(CSB-EL015535HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的NCL含量。核仁素(NCL)是一種多功能蛋白質,廣泛存在于真核細胞中。它參與了核糖體合成、染色質穩定性維持等重要細胞過程。在疾病研究中,NCL與腫瘤發生發展密切相關,可能通過調控基因表達、促進腫瘤細胞增殖和轉移等機制發揮作用,是潛在治療靶點。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,適用于腫瘤分子機制研究、藥物靶點篩選、細胞增殖與凋亡相關實驗等科研場景,特別適合體外培養細胞模型或動物實驗樣本中NCL的功能探索本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    C23 ELISA Kit; FLJ45706 ELISA Kit; MS1116 ELISA Kit; NCL ELISA Kit; Nucl ELISA Kit; NUCL_HUMAN ELISA Kit; Nucleolin ELISA Kit; Protein C23 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.81 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Epigenetics and Nuclear Signaling
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human NCL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %98
    Range %91-105
    1:4Average %99
    Range %92-110
    1:8Average %95
    Range %86-98
  • 回收率:
    The recovery of human NCL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9590-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.675 2.656 2.666 2.542
    10002.312 2.456 1.994 1.870
    5001.709 1.683 1.440 1.316
    2501.047 0.998 0.922 0.798
    1250.617 0.589 0.496 0.372
    62.50.429 0.403 0.415 0.291
    31.250.238 0.221 0.230 0.106
    00.129 0.119 0.124  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清

樣品信息:人

稀釋比:沒有稀釋

產品評價: 我們用CSB-EL015535HU檢測正常人、腎癌、腎炎以及結直腸癌樣本,標曲擬合得很好,正常樣本NCL含量正常,病人樣本中有幾個突出NCL含量較高。

By 吳老師

靶點詳情

  • 功能:
    Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • 基因功能參考文獻:
    1. Data show that NCL phosphorylation offers specificity to its protein-protein, protein-RNA interactions, resulting in the PARN deadenylase regulation, and hence gene expression, during cellular stress responses. PMID: 29168431
    2. Depletion of endogenous NCL in cells by siRNA targeting during H5N1 infection resulted in significantly increased viral polymerase activity. PMID: 29974255
    3. nucleolin interacts with the viral nucleoprotein PMID: 27373907
    4. This review focus on the contribution of nucleolin for cancer disease and on the development of therapeutic strategies targeting this protein. [review] PMID: 29650282
    5. Results found that prostate circulating tumor cells (CTCs) as a population have an increased level of total nucleolin expression compared to white blood cells. Additionally, there was a distinct nucleolin staining pattern and localization in CTCs. PMID: 28153390
    6. this results show that nucleolin increases colony formation and anchorage-independent growth of ErbB2-overexpressing cells, and that nucleolin overexpression in ErbB2-positive breast cancer patients is associated with reduced patient survival rates and increased disease-risk PMID: 27542246
    7. C23 has a role in promoting tumorigenesis via suppressing p53 activity PMID: 27506938
    8. NCL was associated with bipolar disorder. PMID: 28195573
    9. Nucleolin both forms an mRNP complex with the eIF4G and CSF-1 mRNA, and is co-localized with the eIF4G in the cytoplasm further supporting nucleolin's role in translational regulation. PMID: 28131007
    10. Identified the regulator of ribosome production nucleolin (NCL) as over-expressed in AML blasts. Moreover, we found in two series that high NCL mRNA expression level was associated with a poor overall survival, particular in elderly patients. PMID: 28103300
    11. Data suggest a 216-nucleotide proximal cis-element in LIF mRNA exhibits mRNA destabilizing potential; on exposure to carcinogen PMA (phorbol-12-myristate-13-acetate), this cis-element exhibits mRNA stabilizing activity. PMA induces nucleo-cytoplasmic translocation of both nucleolin and PCBP1, 2 trans-acting factors that bind to and stabilize LIF mRNA. [LIF = leukemia inhibitory factor; PCBP1 = poly(rC) binding protein 1] PMID: 28512205
    12. Data suggest that C11orf98 microprotein, NPM1, and nucleolin interact and colocalize in the cell nucleolus. (C11orf98 = chromosome 11 open reading frame 98 protein; NPM1 = nucleophosmin 1) PMID: 28589727
    13. nucleolin inhibition is a new anti-pancreatic cancer therapeutic strategy that dually blocks tumor progression and normalizes tumor vasculature in pancreatic ductal adenocarcinoma PMID: 27754848
    14. It has been found that during microtubule growth phases, nucleolin affects both the speed and life time of microtubule polymerization. PMID: 27309529
    15. Interaction of Host Nucleolin with Influenza A Virus Nucleoprotein in the Early Phase of Infection Limits the Late Viral Gene Expression PMID: 27711134
    16. A yeast display library based on an engineered IgG1 CH2 scaffold with diversified loop regions was constructed, and CH2 binders were isolated by panning against nucleolin. PMID: 28202413
    17. A portion of HS-anMan colocalized with nucleolin at small discrete sites, while most of the nucleolin and nascent RNA was dispersed. In U87 cells, HS-anMan, nucleolin and nascent RNA reassembled after prolonged hypoxia. PMID: 28300561
    18. our findings identified a novel role of NCL in influenza virus life cycle and established NCL as one of the host cell surface proteins for the entry of influenza A virus. PMID: 27085069
    19. C23 on the cell surface may be a kind of indispensable component in activation of EGFR signaling, by which C23 can participate in the growth and invasion of cervical tumors PMID: 26254615
    20. These data suggest that nucleolin could be an effective treatment target and prognostic factor for patients with non-small cell lung cancer PMID: 26846099
    21. These findings demonstrate that nucleolin expression is down-regulated by miR-194 and miR-206 and upregulated by HuR. PMID: 27221739
    22. Nucleolin overexpression is associated with ependymoma. PMID: 26615563
    23. Nucleolin stabilizes oncostatin-M mRNA by binding to a GC-rich element in its 3'UTR. PMID: 26399567
    24. Data show that nucleolin (NCL) antagonist N6L inhibits cell growth with different sensitivity depending to NCL localization. PMID: 26540346
    25. HAUSP-nucleolin interaction is regulated by p53-Mdm2 complex in response to DNA damage. PMID: 26238070
    26. These results suggested a clear link between nucleolin expression (including cell membrane nucleolin) and the stem cell-like phenotype in breast cancer, namely in the triple negative molecular subtype. PMID: 26283155
    27. Identification of NCL as a prominent host factor capable of binding with high affinity to the G4 structures present in the LTR promoter of HIV-1. PMID: 26354862
    28. NCL overexpression is involved in liver carcinogenesis. PMID: 25938538
    29. NCL is implicated in the initiation and transduction of EGFR and CXCR4 signaling in the esophageal squamous cell carcinomas.NCL is expressed in the esophageal squamous cell carcinoma tissues with metastasis in the cell membrane, cytoplasm and nucleus. PMID: 25631630
    30. Data suggest that NCL (nucleolin) is re-localized during lytic KSHV (Kaposi sarcoma-associated herpesvirus) infection and protects IL6 (interleukin-6) mRNA from SOX (KSHV cytoplasmic mRNA-specific endonuclease) degradation in cytoplasm. PMID: 25965334
    31. The involvement of cell surface nucleolin in the initiation of CCR6 signaling in human hepatocellular carcinoma.Expression of nucleolin and CCR6 correlates with overall survival in hepatocellular carcinoma patients. PMID: 25698534
    32. C23 protein meditates bone morphogenetic protein-2-mediated epithelial-to-mesenchymal transition via up-regulation of Erk1/Erk2 and Akt in gastric cancer.Correlations between C23, BMPRII expression and prognosis of gastric cancer patients. PMID: 25698539
    33. study dissects nucleolin-mediated activation of surface AC133 and its cognate gene CD133, via specific interaction of nucleolin with the tissue-dependent CD133 promoter P1 PMID: 26183533
    34. Inhibition of C23 expression was shown to increase the radiosensitivity of NSCLC cells, as implied by the relevance to the notably decreased DNA-PKcs phosphorylation activity at the S2056 and T2609 clusters. PMID: 25921135
    35. the multiple functions of NCL that are associated to its multiple cellular localization can participate to the development of cancer. PMID: 25866190
    36. Nucleolin down-regulation is involved in ADP-induced cell cycle arrest in S phase and cell apoptosis in vascular endothelial cells. PMID: 25290311
    37. This study uncovered a new role for nucleolin in restricting microtubule nucleation and anchoring at centrosomes in interphase cells. PMID: 25590348
    38. C1q exists as the C1 complex (C1qC1r2C1s2), and C1q binding to ligands activates the C1r/C1s proteases. Incubation of nucleoli with C1 caused degradation of the nucleolar proteins nucleolin and nucleophosmin 1. T PMID: 26231209
    39. Increasing expression of nucleolin may be associated with aggressive characteristics of human hepatocellular carcinoma. PMID: 25230759
    40. Lrrc34, a novel nucleolar protein, interacts with npm1 and ncl and has an impact on pluripotent stem cells PMID: 24991885
    41. Data show that nucleolin and endogenous galectin-3 exist in the same complexes in the nucleolus, the cytoplasm, and on the cell surface of melanoma cells. PMID: 25169435
    42. Induced expression of NCL with mutated CK2 phosphorylation sites stabilizes p53, results in higher expression of Bcl2 (B-cell lymphoma 2) homology 3 (BH3)-only apoptotic markers and causes a dominant-negative effect on cell viability. PMID: 25313645
    43. Combined inhibition of nucleolin and ras prevents EGFR activation in glioblastoma cells, additively reducing tumorigenicity. PMID: 25261371
    44. nucleolin may interact with more G protein-coupled receptors, at least chemokine receptor. Our study will lay a new foundation for cancer therapy by antagonizing nucleolin and CXCR4. PMID: 25326811
    45. Studies identify nucleolin as an unconventional epigenetic regulator in leukemia cells and demonstrate nucleolin-NFkappaB-DNMT1 axis as a new molecular pathway underlying AML leukemogenesis. PMID: 25015109
    46. Nucleolin was overexpressed in HCCLM9 cells at the protein and mRNA level. So nucleolin is a novel potential biomarker for the metastasis of hepatocellular carcinoma and a possible therapeutic target for the treatment of hepatocellular carcinoma patients. PMID: 24927373
    47. results for the first time demonstrate that nucleolin-SUMO at K294R plays a critical role in its nucleus sequestration and gadd45alpha mRNA binding activity. PMID: 25561743
    48. this study identified a novel role of the cleavage form of NCL generated by MMP7 in stabilizing MMP9 mRNA. PMID: 24632608
    49. Authors show that the largely nuclear P-protein isoform P3 can localize to nucleoli and forms specific interactions with nucleolin. PMID: 25428867
    50. Transient nucleolar localization of FMRP underlies a strong nucleocytoplasmic translocation, in a complex with nucleolin in order to regulate translation of its target mRNAs. PMID: 24658146

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  • 亞細胞定位:
    Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • 數據庫鏈接:

    HGNC: 7667

    OMIM: 164035

    KEGG: hsa:4691

    STRING: 9606.ENSP00000318195

    UniGene: Hs.79110



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