在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human N-myc-interactor(NMI) ELISA kit

  • 中文名稱:
    人N-Myc互作蛋白(NMI)酶聯免疫試劑盒
  • 貨號:
    CSB-EL015893HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人N-Myc互作蛋白(NMI)酶聯免疫試劑盒(CSB-EL015893HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的NMI含量。N-Myc互作蛋白(NMI)是一種與NMYC和CMYC相互作用的蛋白質,參與細胞增殖、代謝等生物學過程。NMI與NMYC和CMYC家族成員結合,增強STAT介導的轉錄,并在多種癌癥中表達升高,可能成為癌癥治療的新靶點。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL,適用于基礎科研中探索NMI在腫瘤微環境調控、細胞周期異常或免疫逃逸機制中的功能,也可用于評估實驗模型(如基因編輯細胞或動物模型)中NMI的表達動態。為分子機制研究提供可靠的數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    NMI ELISA Kit; N-myc-interactor ELISA Kit; Nmi ELISA Kit; N-myc and STAT interactor ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    23.5 pg/mL-1500 pg/mL
  • 靈敏度:
    5.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Epigenetics and Nuclear Signaling
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human NM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 104
    Range % 100-108
    1:2 Average % 89
    Range % 86-92
    1:4 Average % 106
    Range % 103-109
    1:8 Average % 98
    Range % 94-102
  • 回收率:
    The recovery of human NM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 85 81-89
    EDTA plasma (n=4) 93 90-96
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1500 2.660 2.701 2.681 2.587
    750 2.318 2.203 2.261 2.167
    375 1.676 1.698 1.687 1.593
    187.5 1.065 1.104 1.085 0.991
    94 0.643 0.675 0.659 0.565
    47 0.431 0.442 0.437 0.343
    23.5 0.271 0.281 0.276 0.182
    0 0.093 0.095 0.094  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Acts as a signaling pathway regulator involved in innate immune system response. In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis. Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription. In response to interferon IFN-alpha, associates in a complex with signaling pathway regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35. In complex with IFI35, inhibits virus-triggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21. In complex with IFI35, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Negatively regulates virus-triggered type I interferon/IFN production by inducing proteosome-dependent degradation of IRF7, a transcriptional regulator of type I IFN, thereby interfering with cellular antiviral responses. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by macrophage to the extracellular space during cell injury or pathogen invasion. Macrophage-secreted NMI activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 binding and activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines.
  • 基因功能參考文獻:
    1. These results revealed that IRF-1 is involved in the IFN-inducible expression of Nmi. PMID: 28913576
    2. Our study showed that NMI suppressed tumor growth by inhibiting PI3K/AKT, MMP2/MMP9, COX-2/PGE2 signaling pathways and p300-mediated NF-kappaB acetylation, and predicted a favorable prognosis in human lung adenocarcinomas, suggesting that NMI was a potential tumor suppressor in lung cancer. PMID: 29025423
    3. results provide new insights into understanding the regulatory mechanism of cancer stem cells and suggest that the NMI-YY1-hTERT signaling axis may be a potential therapeutic target for breast cancers. PMID: 28492540
    4. Damage-associated molecular patterns (DAMP) are important mediators of innate immunity. Here the authors show that N-myc and STAT interactor (NMI) and interferon-induced protein 35 (IFP35) act as DAMPs to promote inflammation by activating macrophages via the Toll-like receptor 4 and NF-kappaB pathways. PMID: 29038465
    5. These investigations demonstrated etoposide-induced NMI can suppress tumor proliferation and promote cell apoptosis by activating the ARF-p53 signaling pathway in lung carcinoma. Our results provide an alternative mechanism for etoposide in lung carcinoma and suggest NMI has a critical role in suppressing lung carcinoma progression. PMID: 29030066
    6. Data suggest that N-myc (and STAT) interactor (NMI) could improve its downstream target bradykinin B2 receptor (BDKRB2) expression to induce extracellular signal-regulated kinases (ERK) 1/2 activation, and thereby further evoke malignant progression of hepatocellular carcinoma (HCC). PMID: 28077802
    7. N-Myc-interacting protein (NMI) negatively regulates epithelial-mesenchymal transition by inhibiting the acetylation of NF-kappaB/p65 in histone deacetylase-dependent manner. PMID: 27012186
    8. N-myc and STAT interactor sensitizes breast cancer cells to cisplatin treatment through DRAM1 dependent autophagy. PMID: 26146406
    9. Results show that aberrant miR-29 expression may account for reduced NMI expression in breast tumors and mesenchymal phenotype of cancer cells that promotes invasive growth. PMID: 25174825
    10. The results showed that SARS coronavirus protein 6 can promote the ubiquitin-dependent proteosomal degradation of Nmi. PMID: 25907116
    11. overexpression or depletion of NMI revealed its regulation on G1/S progression and cell proliferation (both in vitro and in vivo), and this effect was partially dependent on STAT1, which interacted with and was regulated by NMI. PMID: 25669971
    12. Trim21 regulates Nmi-IFI35 complex-mediated inhibition of innate antiviral response PMID: 26342464
    13. Its potential function in transcriptional activation of NMI. PMID: 25387807
    14. identified NMI induction as a novel negative feedback mechanism that decreases IRE1alpha-dependent activation of JNK and apoptosis in cytokine-exposed beta cells PMID: 24936061
    15. Thus our work reveals a novel NMI-mediated, transcription-independent ARF induction pathway in response to cellular stresses. PMID: 23034180
    16. Dissociation of the IFN-induced protein NMI from IFP35 is a newly defined specific cytoplasmic event occurring during apoptosis. PMID: 11911807
    17. complex with BRCA1 and c-Myc inhibits c-Myc-induced human telomerase reverse transcriptase gene promoter activity in breast cancer PMID: 11916966
    18. Apoptin mutant T1 still interacted with Nmi, suggesting that its C-terminal 11 AA was not essential for the interaction. PMID: 12019454
    19. STAT1 and Nmi are downstream targets of Ets-1 in MCF-7 human breast cancer cells PMID: 15996661
    20. identified an association of Sox10 with the N-myc interactor Nmi, which was mediated by the high-mobility group of Sox10 and the central region of Nmi; Nmi modulated the transcriptional activity of Sox10 PMID: 16214168
    21. The results provide a novel role of CKIP-1 in cytokine signaling response and the biochemical mechanism, by which two previously identified modulators IFP35 and Nmi are involved via interactions. PMID: 17197158
    22. Activation of PKCdelta in response to Camptothecin treatment requires Myc and is important in CPT-mediated apoptosis signaling. PMID: 17565738
    23. Data suggest that overexpression of Nmi inhibits the Wnt/beta-catenin signaling via up-regulation of Dkk1 and retards tumor growth. PMID: 19358268

    顯示更多

    收起更多

  • 亞細胞定位:
    Cytoplasm. Nucleus. Secreted.
  • 蛋白家族:
    NMI family
  • 組織特異性:
    Expressed in adult spleen, liver, and kidney. Expressed in fetal thymus, liver, placenta, spleen, lung, and kidney but not brain. Expressed in macrophages.
  • 數據庫鏈接:

    HGNC: 7854

    OMIM: 603525

    KEGG: hsa:9111

    STRING: 9606.ENSP00000243346

    UniGene: Hs.54483



主站蜘蛛池模板: 亚洲欧美综合一区二区三区| 99精品久久99久久久久| 色悠久久久久综合欧美99| 国内永久福利在线视频图片| 天天躁夜夜躁狠狠躁2021a2| 99噜噜噜在线播放| 西西人体444www大胆无码视频| 精品国产你懂的在线观看| 无码专区6080yy国产电影| 亚洲成av人无码综合在线观看 | 国产精品vⅰdeoxxxx国产| 国产在线无码制服丝袜无码| 又黄又湿免费高清视频| 国产午夜精品一二区理论影院| 久久久国产一区二区三区| 欧美老妇大p毛茸茸| 国产色视频自在线观看| 久久亚洲精品无码av宋| 国产3p露脸普通话对白| 熟妇丰满多毛的大隂户| 欧美丰满肥婆videos| 大香网伊人久久综合网2018| 一个人看的www片免费高清视频| 丰满岳乱妇在线观看中字无码| 国产精品亚洲精品日韩己满十八小 | 国产精品女主播主要上线| 久久婷婷五月综合鬼色| 欧美大成色www永久网站婷| 成人爱做日本视频免费| 国产av国片精品一区二区| 日产精品久久久久久久性色| 亚洲一区二区三区成人网站| 免费情侣作爱视频| 久久99精品久久久久久蜜芽| av天堂午夜精品一区| 亚洲男人的天堂网站| 综合色区国产亚洲另类| 熟女视频一区二区在线观看| 欧美私人情侣网站| 国产经典三级av在线播放| 最新精品国偷自产在线美女足|