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  4. Human N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Human N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

  • 中文名稱：
    人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶聯免疫試劑盒
  • 貨號：
    CSB-E09450h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶聯免疫試劑盒（CSB-E09450h）為競爭法ELISA試劑盒，定量檢測血清、尿液樣本中的NAG含量。N-乙酰-β-D-氨基葡萄糖苷酶（NAG）是一種溶酶體水解酶，主要存在于近端腎小管，其活性升高是腎小管損傷的早期標志。研究顯示，NAG在腎臟疾病如腎病綜合征、腎盂腎炎等中升高，且其活性變化與疾病嚴重程度相關。通過基因工程和代謝工程，可以優化NAG的合成途徑，提高其產量，為相關疾病的治療提供新的思路。試劑盒檢測范圍為7.81 mIU/mL-2000 mIU/mL，在腎臟疾病研究、藥物毒性評估及生理代謝機制探索中具有重要價值；支持科研人員高效開展腎損傷相關模型構建、疾病機理研究或生物標志物篩選等工作；尤其適用于尿液樣本中NAG活性變化的動態監測，為腎臟功能評估提供可靠實驗工具；助力基礎醫學與生命科學領域的實驗研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Beta hexosaminidase beta chain ELISA Kit; Beta hexosaminidase subunit beta ELISA Kit; Beta N acetylhexosaminidase ELISA Kit; Beta-hexosaminidase subunit beta chain A ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Cervical cancer proto oncogene 7 protein ELISA Kit; Cervical cancer proto-oncogene 7 protein ELISA Kit; ENC 1AS ELISA Kit; Epididymis luminal protein 248 ELISA Kit; HCC 7 ELISA Kit; HCC-7 ELISA Kit; HCC7 ELISA Kit; HEL 248 ELISA Kit; HEX B ELISA Kit; Hexb ELISA Kit; HEXB_HUMAN ELISA Kit; Hexosaminidase B (beta polypeptide) ELISA Kit; Hexosaminidase B ELISA Kit; Hexosaminidase subunit B ELISA Kit; HexosaminidaseB ELISA Kit; N acetyl beta glucosaminidase ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
  • 縮寫：
    NAG
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, urine
  • 檢測范圍：
    7.81 mIU/mL-2000 mIU/mL
  • 靈敏度：
    7.81 mIU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<12%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    95

    Range %

    89-101

    1:2

    Average %

    98

    Range %

    92-106

    1:4

    Average %

    96

    Range %

    88-102

    1:8

    Average %

    90

    Range %

    85-98

  • 回收率：

    The recovery of human NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    86-99

    EDTA plasma (n=4)

    89

    84-94

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    mIU/ml

    OD1

    OD2

    Average

    7.81

    2.263

    2.383

    2.323

    31.25

    1.246

    1.259

    1.253

    125

    0.568

    0.532

    0.550

    500

    0.265

    0.282

    0.274

    2000

    0.146

    0.153

    0.150

  • 本試劑盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with NAG.
    • Standard (2 x 250 μl)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-human NAG Antibody (100 x concentrate) (1 x 60 μl)
    • Antibody Diluent (1 x 10 ml) --Dilute the anti-human NAG antibody.
    • HRP-conjugated goat-anti-mouse NAG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
    • HRP-conjugate Diluent (1 x 20 ml) --dilute the HRP-conjugated NAG antibody.
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
    • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
  • 基因功能參考文獻：
    1. a modified human hexosaminidase subunit beta (HexB), which we have termed mod2B, composed of homodimeric beta subunits that contain amino acid sequences from the alpha subunit that confer GM2 ganglioside-degrading activity and protease resistance. PMID: 27018595
    2. Mutations of the HEXB gene is associated with maple syrup urine disease or Sandhoff disease. PMID: 27682710
    3. report on the heterogeneity of the mutational spectrum of the HEXB gene in Indian patients with Sandhoff disease PMID: 26582265
    4. The absence of beta-N-acetyl-hexosaminidase activity does not alter the differentiation of i-DCs from HSCs, but it is critical for the activation of CD4(+)T cells because knock-down of HEXA or HEXB gene causes a loss of function of i-DCs. PMID: 21997228
    5. Concentration and specific activity of N-acetyl-B-hexosaminidase in palatine tonsils in patients with tonsillar hypertrophy and chronic tonsillitis both in childhood and adulthood significantly increase in comparison to healthy individuals. PMID: 23911049
    6. A total of 19 HEXB variants were found in the 1092 genomes of which 5 are suspected of having a deleterious effect on hexosaminidase activity. PMID: 24461908
    7. DNA from Iranian Tay-Sachs patients reveals a novel mutation in HEXB predicting a termination codon or nonsense mutation. PMID: 24518553
    8. A patient with Sandhoff disease also is found to have a compound macro-deletion in HEXB. PMID: 23886397
    9. A highly significant correlation of HEX-7 and %CDT has been found. Because of exclusion of the P isoform, HEX-7 could be a useful supplementary marker for detecting chronic alcohol abuse. PMID: 23906468
    10. Expression of beta-hexosaminidase in the neurons of Sandhoff disease patients rescues transgenic mice from neurodegeneration. PMID: 22863301
    11. minigene studies revealed the presence of a novel alternative spliced HEXB mRNA variant also present in normal cells PMID: 22848519
    12. Characterization of seven novel mutations on the HEXB gene in French Sandhoff patients. PMID: 23046579
    13. We describe a novel HEXB mutation that is shared among 4 patients with Sandhoff disease. PMID: 22191674
    14. Plasma beta-hexosaminidase and beta-galactosidase) levels are higher in patients with Alzheimer's disease-type 2 diabetes mellitus (T2DM) compared to those with T2DM alone. PMID: 21321400
    15. The X-ray crystal structure of beta-hexosaminidase B provides new insights into mutations that cause Sandhoff disease. PMID: 12706724
    16. novel c.1556A>G transition in exon 12 of the HEXB gene associated with chronic Sandhoff's disease PMID: 17251047
    17. Beta-hexosaminidase is a peptidoglycan hydrolase that surprisingly exerts its mycobactericidal effect at the macrophage plasma membrane during mycobacteria-induced secretion of lysosomes PMID: 18180457
    18. Elevated activity of beta-hexosaminidase observed in subjects with asthma suggests that the beta-hex isozyme could take part in airway inflammation and remodeling in asthma. PMID: 18204279
    19. Lysosome-related genes, such as CLN2, CLN3, and HEXB, may be involved in the pathogenesis of adipose tissue hypertrophy in TED. PMID: 18552385
    20. These results reveal a new aspect of beta-hexosaminidase biology and suggest that a fully processed membrane-associated form of Hex is translocated from the lysosomal membrane to the PM by an as yet unknown mechanism. W PMID: 18588514
    21. Results describe the molecular genetics of Sandhoff disease in Italy and provide new insights into the molecular basis of the disease through HEXB mutation. PMID: 18758829
    22. A new D459A missense HEXB mutation was discovered in six juvenile patients with Sandhoff disease. PMID: 18930675
    23. Gene therapy reduced GM(2) storage and ameliorated neuroinflammation in the brain of HexB(-/-) mice, as well as attenuated behavioral deficits. PMID: 19278737
    24. Data suggest that cigarette smoking can inhibit, by the influence on N-acetyl-beta-hexosaminidase activity, catabolism of oligosaccharide chains in cancer tissues. PMID: 19615986

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  • 相關疾病：
    GM2-gangliosidosis 2 (GM2G2)
  • 亞細胞定位：
    Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
  • 蛋白家族：
    Glycosyl hydrolase 20 family
  • 數據庫鏈接：

    HGNC: 4879

    OMIM: 268800

    KEGG: hsa:3074

    STRING: 9606.ENSP00000261416

    UniGene: Hs.69293



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