在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Human Maternal embryonic leucine zipper kinase(MELK) ELISA kit

  • 中文名稱:
    人母體胚胎亮氨酸拉鏈激酶(MELK)酶聯免疫試劑盒
  • 貨號:
    CSB-EL013692HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人母體胚胎亮氨酸拉鏈激酶(MELK)酶聯免疫試劑盒(CSB-EL013692HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MELK含量。MELK(母體胚胎亮氨酸拉鏈激酶)是一種在多種惡性腫瘤中高表達的蛋白,作為新型分子靶向治療靶點,其通過與多種蛋白相互作用調節靶蛋白,在腫瘤增殖、遷移和干細胞特性維持中起關鍵作用。研究顯示,MELK在神經干細胞和腦腫瘤干細胞自我更新中不可或缺,其功能失調與癌細胞逃避免疫監視有關。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,本產品適用于基礎科研領域,如腫瘤生物學機制研究、干細胞功能探索、抗癌藥物開發中的靶點活性評估等場景,為體外實驗提供可靠的定量檢測工具。實驗操作流程標準化,兼容常規酶標儀檢測,滿足實驗室對多種生物樣本的分析需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    AI327312 ELISA Kit; hMELK ELISA Kit; HPK 38 ELISA Kit; hPK38 ELISA Kit; KIAA0175 ELISA Kit; Likely ortholog of maternal embryonic leucine zipper kinase ELISA Kit; Maternal embryonic leucine zipper kinase ELISA Kit; MELK ELISA Kit; MELK_HUMAN ELISA Kit; mKIAA0175 ELISA Kit; MPK38 ELISA Kit; OTTHUMP00000021377 ELISA Kit; OTTHUMP00000046113 ELISA Kit; pEg3 kinase ELISA Kit; Protein kinase Eg3 ELISA Kit; Protein kinase PK38 ELISA Kit; RP23 382O11.1 ELISA Kit; Tyrosine protein kinase MELK ELISA Kit
  • 縮寫:
    MELK
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human MELK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %86
    Range %80-93
    1:4Average %96
    Range %90-101
    1:8Average %100
    Range %96-105
  • 回收率:
    The recovery of human MELK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9488-99
    EDTA plasma (n=4)9589-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.776 2.863 2.820 2.671
    52.121 1.995 2.058 1.909
    2.51.142 1.241 1.192 1.043
    1.250.705 0.733 0.719 0.570
    0.6250.393 0.387 0.390 0.241
    0.3120.296 0.284 0.290 0.141
    0.1560.238 0.245 0.242 0.093
    00.150 0.148 0.149  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
  • 基因功能參考文獻:
    1. Study demonstrates that the interaction occurring between MELK and EZH2 promotes self-proliferation and stemness. PMID: 28536141
    2. In common culture conditions, the authors found that small molecule inhibition, genetic deletion, or acute depletion of MELK did not significantly affect cellular growth. PMID: 28926338
    3. Synthesis of MCL1, an antiapoptotic protein known to play a role in cancer cell survival during cell division, depends on the function of MELK-elF4B signaling. PMID: 27528663
    4. Inhibition of MELK (genetically and pharmacologically) induces radiation sensitivity. PMID: 27225691
    5. MELK is a host factor required for optimal uncoating of the HIV-1 core to promote viral cDNA synthesis. PMID: 28683086
    6. Here, the authors report that mutagenizing MELK with CRISPR/Cas9 has no effect on the fitness of basal breast cancer cell lines or cell lines from six other cancer types. Cells that harbor null mutations in MELK exhibit wild-type doubling times, cytokinesis, and anchorage-independent growth. PMID: 28337968
    7. MELK-inhibitor has a role in triple-negative breast cancer cells demonstrating context-dependent response with p53 as a key determinant PMID: 28235006
    8. MELK is an oncogenic kinase involved in the pathogenesis and recurrence of hepatocellular carcinoma. PMID: 27693640
    9. Inhibition or depletion of MELK reduced cell proliferation and anchorage-dependent and -independent growth in various ovarian cancer cell lines through a G2/M cell cycle arrest, eventually resulting in apoptosis. PMID: 28214016
    10. Report IL11RA and MELK amplification in gastric cancer cell lines and primary gastric adenocarcinomas. PMID: 27920471
    11. MELK expression in hepatocellular carcinoma is extremely intense compared to its expression reported in other types of cancer and could be a promising effective tumor marker of HCC. PMID: 27798878
    12. targeting MELK by the inhibition of both its catalytic activity and its protein stability might sensitize tumours to DNA-damaging agents or radiation therapy by lowering the DNA-damage threshold PMID: 26431963
    13. Together, these data indicate that MELK is a normally non-essential kinase, but is critical for basal-like breast cancer. PMID: 24844244
    14. EZH2 protects glioma stem cells from radiation-induced cell death in a MELK/FOXM1-dependent manner PMID: 25601206
    15. we report characterization of possible roles of MELK in acute myeloid leukemia PMID: 25365263
    16. insight has been brought by the discovery of a protein complex of FOXM1 with the mitotic kinase MELK in cancer stem cells in brain cancers, as this protein complex appears to be cancer-specific PMID: 25017123
    17. advanced cancers with OTSSP167 started in 2013, as the first-in-class MELK inhibitor. This review summarizes the current molecular understanding of MELK and the recent preclinical studies about MELK as a cancer therapeutic target. PMID: 24795222
    18. MELK promotes cell migration and invasion via the FAK/Paxillin pathway, and plays an important role in the occurrence and development of gastric cancer. PMID: 24885567
    19. our current knowledge of MELK function and recent discoveries in MELK signaling pathway were discussed. PMID: 24185907
    20. The structural and biochemical analyses unravel the molecular mechanisms for the autophosphorylation/activation of MELK and the dependence of its catalytic activity on reducing agents. PMID: 23922895
    21. Report MELK inhibitor that suppresses the growth of a wide range of human tumor cell lines. PMID: 23283305
    22. Data indicate that expression-based risk indices of three genes UBE2C, TPX2, and MELK were more strongly associated with poor 5-year survival in adenocarcinoma patients. PMID: 23357462
    23. MELK is upregulated in high-grade prostate cancer PMID: 22945237
    24. MELK could be associated with increased resistance of colorectal cancer cells against radiation and 5-FU. PMID: 21806965
    25. High MELK is associated with brain tumor. PMID: 21558073
    26. MELK expression is increased in breast cancer tissue and this is associated with poor survival. The most important factors controlling Bcl-G activity are post-translational modification by Fau & MELK. PMID: 19671159
    27. pEg3 is a potential regulator of the G2/M progression and may act antagonistically to the CDC25B phosphatase,pEg3 kinase is able to specifically phosphorylate CDC25B in vitro. One phosphorylation site was identified and corresponded to serine 323 PMID: 12400006
    28. analysis of MELK substrate specificity and activity regulation PMID: 16216881
    29. the kinase activity of MELK is likely to affect mammary carcinogenesis through inhibition of the pro-apoptotic function of Bcl-GL PMID: 17280616
    30. a critical role for MELK in the proliferation of brain tumors, including their stem cells, and suggest that MELK may be a compelling molecular target for treatment of high-grade brain tumors. PMID: 17722061
    31. Maternal embryonic leucine zipper kinase transcript abundance correlates with malignancy grade in astrocytomas PMID: 17960622

    顯示更多

    收起更多

  • 相關疾病:
    Defects in MELK are associated with some cancers, such as brain or breast cancers. Expression is dramatically increased in aggressive undifferentiated tumors, correlating with poor patient outcome in breast and brain cancers, suggesting a role in tumor-initiating cells and proliferation via its function in cell proliferation regulation.
  • 亞細胞定位:
    Cell membrane; Peripheral membrane protein.
  • 蛋白家族:
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
  • 組織特異性:
    Expressed in placenta, kidney, thymus, testis, ovary and intestine.
  • 數據庫鏈接:

    HGNC: 16870

    OMIM: 607025

    KEGG: hsa:9833

    STRING: 9606.ENSP00000298048

    UniGene: Hs.184339



主站蜘蛛池模板: 日本中文一二区有码在线| 人妻去按摩店被黑人按中出| 国产成人精品一区二三区在线观看 | 国产公妇仑乱在线观看| 国产乱人伦av在线a最新| 国产精品一区二区av在线观看| 国产成a人片在线观看视频下载| 麻豆精品一卡二卡三卡| 亚洲中文超碰中文字幕| 国产欧美精品一区二区三区| 亚洲第一se情网站| 久久五十路丰满熟女中出| 国自产拍偷拍精品啪啪av| 国产免费又色又爽粗视频| 欧美精品国产aⅴ一区二区在线| 亚洲a∨国产av综合av网站| 亚洲成年轻人电影网站www| 加勒比久久综合网天天| 国产福利精品一区二区| 少妇人妻中文字幕hd| 欧美另类人妖| 免费无码毛片一区二三区| 亚洲日本va午夜中文字幕久久| 亚洲精品综合欧美一区二区| 国产婷婷色一区二区三区| 99久久精品美女高潮喷水| 色视频无码专区在线观看| 日韩人妻无码精品-专区| 免费国精产品—品二品| 国产成人手机高清在线观看网站 | 久久青草费线频观看| 小宝贝荡货啊用力水湿aⅴ视频 | 亚洲 综合 欧美在线视频| 午夜爱爱爱爱爽爽爽网站| 国产香蕉尹人视频在线| 国产成人综合久久久久久| 2020久久天天躁狠狠躁夜夜| 狠狠躁夜夜躁人人躁婷婷视频| 奇米精品视频一区二区三区| 97se亚洲综合自在线| 欧美性猛交久久久乱大交小说|