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Human Macrophage Migration Inhibitory Factor,MIF ELISA Kit

  • 中文名稱:
    人巨噬細胞移動抑制因子(MIF)酶聯免疫試劑盒
  • 貨號:
    CSB-E08330h
  • 規格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人巨噬細胞移動抑制因子(MIF)酶聯免疫試劑盒(CSB-E08330h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MIF含量。巨噬細胞移動抑制因子(MIF)是一種多效性細胞因子。其在多種生理和病理過程扮演重要角色。研究機制上,它參與炎癥反應、免疫調節等,還與腫瘤發生發展相關,通過多種信號通路發揮作用,是藥物研發等領域的潛在靶點。試劑盒檢測范圍為125 pg/mL-8000 pg/mL,靈敏度為31.25 pg/mL。,適用于探索炎癥相關疾病機制、藥物作用靶點驗證或生物標志物篩選等科研場景,為研究感染性疾病、代謝綜合征或腫瘤免疫調控等領域的科研工作者提供高效檢測方案。MIF參與先天免疫應答、細胞增殖調控及多種疾病如自身免疫性疾病、腫瘤微環境調控等病理過程的研究。血清和血漿樣本便于評估循環系統中MIF的動態變化,而組織勻漿則支持局部組織微環境的研究分析。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    GIF ELISA Kit; GLIF ELISA Kit; Glycosylation inhibiting factor ELISA Kit; Glycosylation-inhibiting factor ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; Macrophage migration inhibitory factor (glycosylation-inhibiting factor) ELISA Kit; Macrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; MIF protein ELISA Kit; MIF_HUMAN ELISA Kit; MMIF ELISA Kit; Phenylpyruvate tautomerase ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
  • 檢測范圍:
  • 靈敏度:
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    1040.655

    1084.605

    1112.932

    1024.991

    1087.750

    1144.474

    SD

    0.02

    0.023

    0.025

    0.024

    0.026

    0.028

    CV(%)

    4.167

    4.656

    4.970

    5.053

    5.253

    5.458

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:5

    Average %

    85

    Range %

    80-90

    1:10

    Average %

    92

    Range %

    87-96

    1:20

    Average %

    85

    Range %

    82-92

    1:40

    Average %

    96

    Range %

    90-102

  • 回收率:

    The recovery of human MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    98

    90-105

    Platelet-poor EDTA plasma (n=4)

    96

    87-103

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    8000

    2.126

    2.052

    2.089

    1.977

    4000

    1.359

    1.276

    1.318

    1.206

    2000

    0.784

    0.734

    0.759

    0.647

    1000

    0.459

    0.439

    0.449

    0.337

    500

    0.348

    0.324

    0.336

    0.224

    250

    0.256

    0.238

    0.247

    0.135

    125

    0.181

    0.171

    0.176

    0.064

    0

    0.115

    0.109

    0.112

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展:
    MIF是一種促炎癥因子,全名為遷移抑制因子。它參與免疫反應、炎癥反應、細胞增殖等多種生理過程,在多種疾病的發生和發展中發揮重要作用。最近的研究表明,MIF與腫瘤、炎癥性疾病、自身免疫性疾病等多種疾病的發生和發展密切相關,
  • 功能:
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 基因功能參考文獻:
    1. These results highlight the importance of MIF overexpression in pancreatic ductal adenocarcinoma aggressiveness, and indicate that MIF may be a potential therapeutic target for pancreatic cancer PMID: 30226561
    2. MIF serum levels are elevated in erythema nodosum leprosum patients as compared to controls. PMID: 28656911
    3. Overall, the present work showed that MIF is a shear stress-sensitive cytokine and is transcriptionally regulated by KLF2, suggesting that laminar shear stress exerts its athero-protective effect in part by directly inhibiting pro-inflammatory MIF expression. PMID: 29403061
    4. Hypoxia-induced angiogenesis is a complex process that involves distinct but also overlapping functions of HIF-1alpha and HIF- 2alpha in regard to angiogenesis, bioenergetic adaption and the redundant transcriptional induction of MIF. PMID: 28993199
    5. that macrophage migration inhibitory factor directly engages in dengue NS1-induced glycocalyx degradation and that targeting MIF may represent a possible therapeutic approach for preventing dengue-induced vascular leakage PMID: 29702687
    6. In this study, we conclude that the CC ge-notype of the MIF -173GC variant may be a risk factor in the pathogenesis of Behcet's Disease in the Turkish population. However, further studies with larger samples are needed to address the exact role of this variant in BD. PMID: 29669352
    7. Study describes a brain glycation profile and identify MIF, an immune regulator and insulin regulator, as being glycated and oxidised in Alzheimer's Disease (AD) brain homogenates. The glycation completely inhibited the oxidoreductase activity of MIF, and severely attenuated its tautomerase activity. PMID: 28230058
    8. our results reveal that MIF expression is a significant independent prognostic factor for patients with oral squamous cell carcinoma PMID: 30041958
    9. the expression of MMIF, HIF-1alpha, and VEGF in the serum and endometrial tissues may be used to assess the stage of endometriosis and the severity of dysmenorrhea. PMID: 30074218
    10. migration inhibitory factor can aggravate diabetic neuropathy by suppressing glyoxalase-I and intraepidermal nerve fibers on the footpad skin lesions and provoke pain. PMID: 29690804
    11. This study provides better understanding of the mechanisms and disease susceptibility for MIF and CD14 genetic variants and inflammatory miRNAs networks involved in ankylosing spondylitis and polyarthralgia. PMID: 29863307
    12. In this review, we summarize and discuss MIF post-translational modifications and their effects on the biological properties of this protein. We propose that the redox-sensitive residues in MIF will be modified at sites of inflammation and that this will add further depth to the functional diversity of this intriguing cytokine PMID: 29247897
    13. meta-analyses revealed significantly higher circulating MIF levels in patients with rheumatoid arthritis, and found evidence of associations between the MIF-173 C/G and -794CATT5-8 polymorphisms and rheumatoid arthritis susceptibility PMID: 28912189
    14. MIF -794 CATT genotypes 7/8 and allele CATT 8 were highly associated with pulmonary tuberculosis in a southwestern China population. PMID: 29809055
    15. Results found MIF and TNFA mRNA expression increased in psoriatic arthritis (PsA) patients with high activity in relation to patients with moderate activity. These results suggest that both mRNA possibly have a role in the progress of the disease severity. PMID: 28965181
    16. The present study indicated that the MIF-173G/C polymorphism may contribute to the development of pulmonary tuberculosis. Furthermore, significantly higher serum MIF levels were observed in pulmonary tuberculosis patients than in controls, which further indicated that the MIF may play an important role in pulmonary tuberculosis progression, particularly in Asians. PMID: 28331211
    17. MIF and IL-6 play important roles in the inflammation associated with polymyositis. PMID: 29239244
    18. The expression collagen type 3, alpha1 (Col3A1), matrix metalloproteinase (MMP)2/-9 and transforming growth factor (TGF)beta was higher in patients with permanent atrial fibrillation (AF), compared with patients in sinus rhythm (SR), and the expression levels of MIF were also increased in AF. MIF was involved in the structural remodeling that accompanies AF, possibly by promoting the proliferation of cardiac fibroblasts. PMID: 29257298
    19. the findings of the present study demonstrated that MIF is a key component in lung cancer progression through promoting the Warburg effect, and that the novel MIF/NFkappaB/HIF1alpha axis may prove to be useful for the development of new strategies for treating patients with lung cancer PMID: 29207023
    20. These results potentially support an effect of MIF, but not Type I IFN, in heightened systemic lupus erythematosus disease severity in Asian systemic lupus erythematosus. The associations of MIF and Asian ethnicity with PAD are at least partly independent PMID: 27453287
    21. High MIF expression is associated with progressive multiple sclerosis. PMID: 28923927
    22. Increased perioperative MIF-levels are related to an increased risk of adverse outcome in complex aortic surgery and may represent a biomarker for risk stratification in complex aortic surgery. PMID: 29120365
    23. No association showed between SLE and the MIF -173 C allele, the -794CATT7 allele, and the -794CATT7-MIF-173C haplotype with high heterogeneity. PMID: 29096759
    24. Studied correlation of serum macrophage migration inhibitory factor (MIF) and atrial fibrillation (AF) patients and healthy subjects; found higher serum MIF concentrations in AF patients than in healthy subjects. PMID: 28407372
    25. genetic polymorphisms of MIF may increase the risk of posttransplant development of donor specific antibodies in kidney allograft recipients PMID: 28624489
    26. We reviewed the association of the MIF rs755622*C allele with systemic sclerosis, and described a phenotype-specific association of this variant with the susceptibility to develop pulmonary arterial hypertension in patients with diffuse cutaneous systemic sclerosis. PMID: 28668810
    27. Authors report that cysteine 81 within MIF serves as a "switch cysteine" for the conversion of redMIF to oxMIF. Modulating cysteine 81 by thiol reactive agents leads to significant structural rearrangements of the protein, resulting in a decreased beta-sheet content and an increased random coil content, but maintaining the trimeric quaternary structure. PMID: 29412660
    28. These findings predict the binding mode of Hu-MIF-1 and orthologs with CD74. PMID: 28513076
    29. Serum MIF provides information about inflammation, brain injury severity and outcome after aSAH PMID: 28823650
    30. MIF in serum might be a potential biomarker for reflecting inflammation, severity and prognosis of ICH patients PMID: 28729134
    31. In conclusion, MIF rs755622 polymorphism increases breast cancer susceptibility in Chinese population, especially among elder patients. PMID: 27844180
    32. MIF coreceptor CD44 is expressed by rheumatoid synovial fibroblasts in a MIF allele-dependent fashion and undergoes functional regulation and activation by autocrine/paracrine MIF PMID: 27872288
    33. High MIF expression is associated with hepatocellular injury and graft rejection. PMID: 28370484
    34. MIF is involved in the pathogenesis of AF, probably by down-regulating the protein and gene expression of Cx43 via ERK1/2 kinase activation PMID: 28429502
    35. Lower circulating migration inhibitory factor protein is associated with metabolic syndrome and diabetes PMID: 28703031
    36. Increased serum MIF levels are associated with the elevation of pre-diabetic risk. PMID: 29289260
    37. Serum macrophage migratory inflammatory factor levels were higher in HD patients (982.74 pg/mL) than that of PD patients (762.20 pg/mL), CKD patients (755.66 pg/mL), or healthy controls (336.81 pg/mL) (P = 0.009). Levels were also significantly increased in patients with left ventricular hypertrophy, and they correlated with the levels of other inflammatory factors. PMID: 27811451
    38. The assay monitors the increase in absorbance at 320 nm resulting from keto-to-enol tautomerization of 4-hydroxyphenylpyruvate, a reaction catalyzed by MIF. We ran a full-diversity screen evaluating the inhibitory activity of 1.6 million compounds. PMID: 26933127
    39. The rs5844572 polymorphism in MIF is linked to the rate of progression and extent of fibrosis in Biliary Atresia Patients, but not with disease susceptibility. PMID: 28657145
    40. Absolute numbers of MIF and IL-1beta mRNA molecules are both accurate and reliable predictors of antidepressant response. PMID: 27207917
    41. The knockdown of D-DT and MIF, individually and additively, inhibited the proliferation, migration, and invasion in HeLa and SiHa cells and restrained the growth of xenograft tumor. PMID: 28338494
    42. No association between coronary artery disease class and -794 CATT5-8 MIF polymorphisms with soluble MIF levels in CAD subjects. PMID: 28578664
    43. These results have implications for the manner in which D-DT and MIF compete with each other for binding to the CD74 receptor and for the relative potency of DRa1-MOG-35-55 and RTL1000 for competitive inhibition of D-DT and MIF binding and activation through CD74. PMID: 27573366
    44. Plasma MIF was elevated after cardiac stress (relative to before stress) in patients with a positive stress test, compared with those with a negative stress test. Plasma MIF is an early marker for myocardial ischemia. MIF was not altered after exercise in peripheral arterial occlusive disease patients, despite the occurrence of claudication, suggesting that plasma MIF is not a marker for skeletal muscle ischemia. PMID: 27364992
    45. Increased serum MIF concentrations have close relation to inflammation, trauma severity and clinical outcomes, substantializing MIF as a good prognostic biomarker after TBI PMID: 28366792
    46. renal tubular MIF is an endogenous renoprotective factor in progressive kidney diseases PMID: 28801314
    47. involved in the pathogenesis of idiopathic pulmonary fibrosis being mainly expressed in the areas of remodeling and active fibrosis, in bronchiolar and alveolar epithelium, and in the peripheral zone of fibroblastic foci PMID: 27337548
    48. MIF is primarily an indirect promoter of glioblastoma progression. PMID: 27145382
    49. frequent upregulation of MIF is implicated in the development and progression ofesophageal squamous cell carcinoma (ESCC). PMID: 29079416
    50. Data highlight the complexity of the MIF/CD74 signaling pathway in the development of mesothelioma. PMID: 26883190

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  • 相關疾病:
    Rheumatoid arthritis systemic juvenile (RASJ)
  • 亞細胞定位:
    Secreted. Cytoplasm.
  • 蛋白家族:
    MIF family
  • 數據庫鏈接:

    HGNC: 7097

    OMIM: 153620

    KEGG: hsa:4282

    STRING: 9606.ENSP00000215754

    UniGene: Hs.407995



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