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  4. Human Liver-expressed antimicrobial peptide 2(LEAP2) ELISA kit

Human Liver-expressed antimicrobial peptide 2(LEAP2) ELISA kit

  • 中文名稱：
    人肝表達抗菌肽2(LEAP2)酶聯免疫試劑盒
  • 貨號：
    CSB-EL012853HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人肝表達抗菌肽2(LEAP2)酶聯免疫試劑盒（CSB-EL012853HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LEAP2含量。人肝表達抗菌肽2（LEAP2）是一種新型活性多肽，具有抑菌和調節體重功能。其結構穩定，在先天免疫系統中發揮關鍵作用，能抵御細菌感染，并參與調節食欲、能量代謝等生理過程。LEAP2通過破壞細菌細胞膜和調節免疫細胞功能來發揮其抗菌和免疫調節作用。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，支持科研人員探索LEAP2在代謝性疾病模型中的動態變化、評估肝臟功能狀態，或研究其與微生物感染相關的免疫調節機制。為肝臟代謝研究、免疫調控機制探索及藥物開發提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    LEAP2Liver-expressed antimicrobial peptide 2 ELISA Kit; LEAP-2 ELISA Kit
  • 縮寫：
    LEAP2
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.312 ng/mL-20 ng/mL
  • 靈敏度：
    0.078 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human LEAP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 105
    Range % 98-111
    1:2 Average % 99
    Range % 90-104
    1:4 Average % 97
    Range % 86-103
    1:8 Average % 103
    Range % 95-107
  • 回收率：
    The recovery of human LEAP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 91-104
    EDTA plasma (n=4) 102 95-107
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.512 2.600 2.556 2.403
    10 2.101 1.978 2.040 1.887
    5 1.403 1.415 1.409 1.256
    2.5 0.824 0.844 0.834 0.681
    1.25 0.489 0.501 0.495 0.342
    0.625 0.311 0.323 0.317 0.164
    0.312 0.231 0.226 0.229 0.076
    0 0.156 0.150 0.153 　
  • 本試劑盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human LEAP2 antibody.
    • Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial HRP-conjugated LEAP2 antibody (6 ml/bottle) ---Bind to the MSTN in the samples or standards and react with the substrate to make the solution chromogenic.
    • Substrate A (1 x 7 ml)
    • Substrate B (1 x 7 ml)
    • One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
    • An instruction manual

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  • 本試劑盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Has an antimicrobial activity.
  • 基因功能參考文獻：
    1. We identified the amphibian leap2 gene which is highly related to its mammalian orthologues at both structural and sequence levels. The gene is expressed in the embryo mostly in the endoderm-derived tissues. Accordingly it is induced in pluripotent animal cap cells by FGF, activin or a combination of vegT/beta-catenin. PMID: 27335344
    2. LEAP-2 has a novel fold for a cationic antimicrobial peptide and has antibacterial activity. LEAP-2 exhibits antimicrobial activity under low-salt conditions, and there is likely to be another physiological role for the peptide. PMID: 20845358
    3. LEAP-2 displays antimicrobial activity against bacteria and yeasts. PMID: 20845358
    4. These results show that the secondary structure of human LEAP-2 has a profound impact on its antibacterial activity. PMID: 19852990
    5. Data suggest that LEAP-2 is not involved in the physiological response of GI epithelia to iron, nor is it mitogenic for epithelial cells or chemotactic for THP-1 monocytes. PMID: 20038463

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    LEAP2 family
  • 數據庫鏈接：

    HGNC: 29571

    OMIM: 611373

    KEGG: hsa:116842

    UniGene: Hs.337588



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