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Human Leucine-rich repeat-containing protein 17(LRRC17) ELISA kit

  • 中文名稱:
    人富亮氨酸重復(fù)序列蛋白17(LRRC17)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL013112HU
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人富亮氨酸重復(fù)序列蛋白17(LRRC17)酶聯(lián)免疫試劑盒(CSB-EL013112HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的LRRC17含量。試劑盒檢測范圍為23.44 pg/mL-1500 pg/mL,該產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域,可用于探究LRRC17在腫瘤微環(huán)境、免疫調(diào)節(jié)或神經(jīng)退行性疾病中的表達(dá)變化,為相關(guān)分子機(jī)制研究提供定量分析工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    LRRC17 ELISA Kit; P37NB ELISA Kit; UNQ3076/PRO9909 ELISA Kit; Leucine-rich repeat-containing protein 17 ELISA Kit; p37NB ELISA Kit
  • 縮寫:
    LRRC17
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    23.44 pg/mL-1500 pg/mL
  • 靈敏度:
    5.86 pg/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human LRRC17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %95
    Range %90-101
    1:2Average %92
    Range %85-98
    1:4Average %86
    Range %80-92
    1:8Average %94
    Range %90-100
  • 回收率:
    The recovery of human LRRC17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-99
    EDTA plasma (n=4)9591-101
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15001.711 1.672 1.692 1.562
    7501.426 1.396 1.411 1.281
    3751.012 1.004 1.008 0.878
    187.50.775 0.756 0.766 0.636
    93.750.583 0.544 0.564 0.434
    46.870.374 0.359 0.367 0.237
    23.440.262 0.247 0.255 0.125
    00.133 0.127 0.130  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Involved in bone homeostasis. Acts as a negative regulator of RANKL-induced osteoclast precursor differentiation from bone marrow precursors.
  • 基因功能參考文獻(xiàn):
    1. low circulating plasma level of LRRc17 might serve as an independent and additive risk factor for osteoporotic fractures. PMID: 27355564
    2. Whole-genome microarray mRNA-expression profiles of myofibroblasts and skin fibroblasts revealed four additional genes that are significantly differentially expressed in these two cell types: NKX2-3 and LRRC17 in myofibroblasts and SHOX2 and TBX5 in skin fibroblasts PMID: 27036009
  • 亞細(xì)胞定位:
    Secreted, extracellular space.
  • 組織特異性:
    Expressed in osteoblast cell lines. Well expressed in ovary, heart, pancreas, skeletal muscle, lung, and fetal kidney and lung and only at the basal levels in the other tissues examined including adult kidney. More expressed in S-type neuroblastoma cells
  • 數(shù)據(jù)庫鏈接:

    HGNC: 16895

    KEGG: hsa:10234

    STRING: 9606.ENSP00000344242

    UniGene: Hs.567412



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