Human Keratin, type I cytoskeletal 23(KRT23) ELISA kit

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Human Keratin, type I cytoskeletal 23(KRT23) ELISA kit

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中文名稱：

人角蛋白I型細(xì)胞骨架23(KRT23)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL012539HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人角蛋白I型細(xì)胞骨架23(KRT23)酶聯(lián)免疫試劑盒（CSB-EL012539HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的KRT23含量。KRT23 作為一個(gè)靶點(diǎn)，目前可能在醫(yī)學(xué)等領(lǐng)域具有一定研究?jī)r(jià)值。它屬于角蛋白相關(guān)靶點(diǎn)。相關(guān)研究機(jī)制或許圍繞其在細(xì)胞生長(zhǎng)、分化、代謝等過(guò)程中的作用展開，探索其在疾病發(fā)生發(fā)展中的角色，以尋求可能的治療干預(yù)靶點(diǎn)。試劑盒檢測(cè)范圍為0.9 ng/mL-60 ng/mL，適用于基礎(chǔ)醫(yī)學(xué)研究中KRT23蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)，例如肝纖維化病理機(jī)制探索、腫瘤微環(huán)境分析或藥物干預(yù)效果評(píng)估等科研場(chǎng)景；可用于體外檢測(cè)細(xì)胞培養(yǎng)上清、動(dòng)物模型或人體組織樣本中的KRT23含量，為相關(guān)疾病的分子機(jī)制研究提供實(shí)驗(yàn)工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

CK 23 ELISA Kit; CK-23 ELISA Kit; CK23 ELISA Kit; Cytokeratin 23 ELISA Kit; Cytokeratin-23 ELISA Kit; Cytokeratin23 ELISA Kit; DKFZP434G032 ELISA Kit; HAIK 1 ELISA Kit; HAIK1 ELISA Kit; Histone deacetylase inducible keratin 23 ELISA Kit; Hyperacetylation inducible type I keratin ELISA Kit; K1C23_HUMAN ELISA Kit; K23 ELISA Kit; Keratin 23 (histone deacetylase inducible) ELISA Kit; Keratin 23 ELISA Kit; Keratin ELISA Kit; Keratin type I cytoskeletal 23 ELISA Kit; Keratin-23 ELISA Kit; Keratin23 ELISA Kit; KRT 23 ELISA Kit; KRT23 ELISA Kit; MGC26158 ELISA Kit; type I cytoskeletal 23 ELISA Kit; Type I intermediate filament cytokeratin ELISA Kit
縮寫：

KRT23
Uniprot No.：

Q9C075
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.9 ng/mL-60 ng/mL
靈敏度：

0.23 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human KRT23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	91
	Range %	87-96
1:4	Average %	88
	Range %	82-94
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of human KRT23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-102
EDTA plasma (n=4)	93	87-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.761	2.562	2.662	2.537
30	2.155	2.062	2.109	1.984
15	1.337	1.305	1.321	1.196
7.5	0.745	0.720	0.733	0.608
3.75	0.413	0.405	0.409	0.284
1.8	0.276	0.283	0.280	0.155
0.9	0.193	0.189	0.191	0.066
0	0.125	0.124	0.125

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.