Human Keratin, type I cytoskeletal 18 fragment (K18-Asp396)ELISA Kit
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中文名稱:人細(xì)胞角蛋白18片段Asp396(K18-Asp396)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E13696h
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:人細(xì)胞角蛋白18片段Asp396(K18-Asp396)酶聯(lián)免疫試劑盒(CSB-E13696h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物.樣本中的CK 18M30含量。試劑盒檢測(cè)范圍為15.6 mIU/ml-1000 mIU/ml,適用于體外研究細(xì)胞凋亡信號(hào)通路、評(píng)估藥物肝毒性引起的上皮細(xì)胞損傷、探索炎癥性疾病中組織修復(fù)機(jī)制等科研實(shí)驗(yàn)本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名:N/A
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縮寫:CK 18M30
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates, cell lysates.
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檢測(cè)范圍:15.6 mIU/ml-1000 mIU/ml
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靈敏度:3.9 mIU/ml
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 85-94 1:2 Average % 86 Range % 81-92 1:4 Average % 94 Range % 90-99 1:8 Average % 89 Range % 83-96 -
回收率:
The recovery of human CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 90-100 EDTA plasma (n=4) 99 94-105 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. mIU/ml OD1 OD2 Average Corrected 1000 2.577 2.552 2.565 2.443 500 2.056 2.016 2.036 1.914 250 1.267 1.202 1.235 1.113 125 0.683 0.648 0.666 0.544 62.5 0.379 0.405 0.392 0.270 31.2 0.294 0.279 0.287 0.165 15.6 0.201 0.199 0.200 0.078 0 0.117 0.126 0.122 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- CYTOKERATIN CK 18 (M30): A Novel Biomarker in Acute Liver Failure C Malik,Hepatology research,2023
- The efficacy of serum cell death biomarkers for diagnosing biliary tract cancer Sugimoto M, et al,Scientific Reports,2018
- The Utility of M30 and M65 Antigen Concentration Levels for Predicting Degree of Hepatic Injury in Patients with Chronic Hepatitis B Infection Mustafa Celik, et al,/,2018
- Serum levels of a cell death biomarker predict the development of cirrhosis-related conditions in primary biliary cholangitis Hayashi M.et al,Med Mol Morphol,2018
- No effects of oral vitamin D supplementation on non-alcoholic fatty liver disease in patients with type 2 diabetes: a randomized, double-blind, placebo-controlled trial. Barchetta I.et al,BMC Med.,2016
- Fish Oil Supplements Lower Serum Lipids and Glucose in Correlation with a Reduction in Plasma Fibroblast Growth Factor 21 and Prostaglandin E2 in Nonalcoholic Fatty Liver Disease Associated with Hyperlipidemia: A Randomized Clinical Trial. Qin Y. et al,PLoS One,2015
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相關(guān)問答
請(qǐng)問人細(xì)胞角蛋白18片段Asp396(K18-Asp396)ELISA Kit(CSB-E13696h)是評(píng)估細(xì)胞凋亡的試劑盒嗎?評(píng)估總細(xì)胞死亡(凋亡+壞死)的補(bǔ)充指標(biāo)是什么?
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