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Human KIAA1199 ELISA Kit

  • 中文名稱:
    人 KIAA1199酶聯免疫試劑盒
  • 貨號:
    CSB-E13092h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人 KIAA1199酶聯免疫試劑盒(CSB-E13092h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CEMIP含量。CEMIP(細胞外基質金屬蛋白酶誘導因子結合蛋白)在多種生物學過程中發揮作用。其研究機制顯示它與細胞遷移、侵襲及基質重塑相關,在腫瘤發生發展里或扮演重要角色,會影響腫瘤的生長、轉移等,是腫瘤治療領域備受關注的潛在靶點。試劑盒檢測范圍為1 ng/mL-24 ng/mL,適用于基礎醫學研究領域,例如探究KIAA1199在腫瘤微環境調控、炎癥反應或創傷修復中的作用機制,也可用于篩選與細胞遷移相關的藥物靶點或評估疾病模型中的生物標志物表達變化,為腫瘤生物學和病理學研究提供重要工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    CEMIP ELISA Kit; KIAA1199 ELISA Kit; Cell migration-inducing and hyaluronan-binding protein ELISA Kit; EC 3.2.1.35 ELISA Kit
  • 縮寫:
    KIAA1199
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1 ng/mL-24 ng/mL
  • 靈敏度:
    0.4 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<10%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human KIAA1199 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-105
    1:2Average %85
    Range %81-89
    1:4Average %101
    Range %99-103
    1:8Average %87
    Range %81-92
  • 回收率:
    The recovery of human KIAA1199 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-98
    EDTA plasma (n=4)10498-108
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    242.575 2.634 2.605 2.563
    121.455 1.374 1.415 1.373
    60.703 0.718 0.711 0.669
    20.222 0.235 0.229 0.187
    10.111 0.116 0.114 0.072
    00.041 0.042 0.042
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.
  • 基因功能參考文獻:
    1. the in-vitro anti-proliferative and pro-apoptotic effects in colorectal cancer cells that were induced by silencing cell migration inducing hyaluronan binding protein may be associated with GRP78 repression and UPR attenuation PMID: 29024602
    2. CEMIP expression was increased in retinoblastoma tissues and cells. miR-140-5p inhibited CEMIP expression possibly by targeting the 3'-UTR. PMID: 29808799
    3. KIAA1199 is overexpressed in pancreatic intraepithelial neoplasia. PMID: 27922049
    4. Our findings revealed that KIAA1199 protein could be applied in predicting nonsmall cell lung cancer patient's outcome PMID: 28901311
    5. Overexpression of KIAA1199 may contribute to increased migration of pancreatic ductal adenocarcinoma cells and predict shorter survival after surgical resection. PMID: 28012880
    6. Results suggest a possible link between inflammation, induced KIAA1199 expression, and enhanced migration during pancreatic ductal adenocarcinoma (PDAC) progression. PMID: 28179576
    7. Elevated KIAA1199 protein expression is associated with tumor invasion and metastasis in colorectal cancer. PMID: 28213952
    8. KIAA1199 promotes migration and invasion by Wnt/beta-catenin pathway and matrix metalloproteinase-mediated epithelial-mesenchymal transition progression, and serves as a poor prognosis marker in gastric cancer. PMID: 28422983
    9. CEMIP directly facilitates colon tumor growth, and high CEMIP expression correlates with poor outcome in stage III and in stages II+III combined cohorts. PMID: 26437221
    10. Results provide insight into the upregulation of CEMIP within cancer and can lead to novel treatment strategies targeting this cancer cell migration-promoting gene. PMID: 26009875
    11. We present evidence that high expression of KIAA1199 is associated with tumor invasion depth, TNM stage, and poor prognosis in colorectal cancer PMID: 26045799
    12. Regulation of Hyaluronan (HA) Metabolism Mediated by HYBID (Hyaluronan-binding Protein Involved in HA Depolymerization, KIAA1199) and HA Synthases in Growth Factor-stimulated Fibroblasts. PMID: 26518873
    13. oncogenic protein induced by HPV infection and constitutive NF-kappaB activity that transmits pro-survival and invasive signals through EGFR signalling PMID: 25366117
    14. KIAA1199 plays an important role in glycogen breakdown and cancer cell survival PMID: 25051373
    15. KIAA1199 influences the proliferation, adhesion, motility, invasiveness and epithelial-to-mesenchymal transition of cancer cells, is a likely target gene of the Wnt/beta-catenin signalling pathway[review] PMID: 24573670
    16. Findings indicate that KIAA1199 may play an important role in breast tumor growth and invasiveness. PMID: 24628760
    17. proteins that might interact with KIAA1199 and molecular pathways in which it might play roles, were identified. PMID: 23936024
    18. Results suggest that the N-terminal portion of KIAA1199 functions as a cleavable signal sequence required for proper KIAA1199 translocation and KIAA1199-mediated HAhyaluronan depolymerization. PMID: 24269685
    19. KIAA1199 serves as a novel cell migration-promoting gene and plays a critical role in maintaining cancer mesenchymal status. PMID: 23990668
    20. KIAA1199 is a new hyaluronan binding protein involved in hyaluronan depolymerization. PMID: 23509262
    21. KIAA1199 is differentially expressed in neoplastic tissues and KIAA1199 transcripts are more abundant in the plasma of patients with either cancer or adenoma compared to controls. PMID: 22276102
    22. KIAA1199 transcript and protein are highly expressed in the majority of CRCs. KIAA1199 probably participates in Wnt-signalling, affecting cell proliferation, motility, adhesion. Also, KIAA1199 has a clinical correlation to outcome in stage-II CRC patients PMID: 21772334
    23. KIAA1199 is upregulated in gastric adenocarcinoma and is associated with tumor progression. PMID: 21443102
    24. three possibly disease-causing point mutations PMID: 14577002
    25. KIAA1199 was highly expressed in gastric cancer, and was associated with prognosis and lymph node metastasis in multivariate analyses. Taken together, KIAA1199 may be a novel gene that plays an important role in progression of gastric cancer. PMID: 19434458

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  • 亞細胞定位:
    Nucleus. Cytoplasm. Endoplasmic reticulum. Cell membrane. Membrane, clathrin-coated pit. Secreted. Note=Retained in the endoplasmic reticulum (ER) in a HSPA5/BIP-dependent manner. Colocalized with clathrin heavy chain/CLTC in clathrin-coated vesicles. Strongly detected in the cytoplasm of breast carcinoma cells, whereas poorly detected in adjacent normal epithelial cells, stromal cells, or benign breast tissues. Localized in the nucleus and cytoplasm of colon adenocarcinomas.
  • 蛋白家族:
    TMEM2 family
  • 組織特異性:
    Expressed in dermal and in synovial fibroblasts. Strongly expressed in gastric cancers compared with the paired normal tissues. Strongly expressed in both ductal carcinoma and invasive breast cancer cells compared with benign epithelial cells (at protein
  • 數據庫鏈接:

    HGNC: 29213

    OMIM: 608366

    KEGG: hsa:57214

    STRING: 9606.ENSP00000220244

    UniGene: Hs.459088



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