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  4. Human Interleukin17B (IL17B) ELISA Kit

Human Interleukin17B (IL17B) ELISA Kit

  • 中文名稱：
    人白介素17B (IL17B)酶聯免疫試劑盒
  • 貨號：
    CSB-E15918h
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人白介素17B (IL17B)酶聯免疫試劑盒（CSB-E15918h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL17B含量。IL-17B 是一種細胞因子。其它在免疫調節中起作用。研究機制上，IL-17B 與相應受體結合，激活下游信號通路，參與炎癥反應、組織修復等過程，對其深入研究有助于理解免疫相關疾病發病機制，為治療提供新方向。試劑盒檢測范圍為31.25 pg/ml-2000 pg/ml.，靈敏度為7.8 pg/mL。適用于基礎科研中探索IL17B在免疫調控、炎癥性疾病模型或腫瘤微環境中的作用機制，也可用于體外藥物篩選實驗中生物標志物的動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Cytokine like protein Zcyto7 ELISA Kit; Cytokine Zcyto7 ELISA Kit; IL 17B ELISA Kit; IL 20 ELISA Kit; IL-17B ELISA Kit; IL-20 ELISA Kit; IL17B ELISA Kit; IL17B_HUMAN ELISA Kit; IL20 ELISA Kit; Interleukin 17 beta ELISA Kit; Interleukin 17B ELISA Kit; Interleukin 20 ELISA Kit; Interleukin-17B ELISA Kit; Interleukin-20 ELISA Kit; MGC138900 ELISA Kit; MGC138901 ELISA Kit; Neuronal interleukin 17 related factor ELISA Kit; Neuronal interleukin-17-related factor ELISA Kit; NIRF ELISA Kit; ZCYTO7 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/ml-2000 pg/ml.
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(pg/ml)

    252.019

    260.431

    227.817

    252.542

    239.054

    254.114

    SD

    0.034

    0.028

    0.032

    0.045

    0.049

    0.042

    CV(%)

    5.015

    4.035

    5.071

    6.627

    7.504

    6.158

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL17B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    106

    Range %

    103-109

    1:2

    Average %

    93

    Range %

    92-98

    1:4

    Average %

    92

    Range %

    86-96

    1:8

    Average %

    94

    Range %

    89-97

  • 回收率：

    The recovery of human IL17B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    103

    99-109

    EDTA plasma (n=4)

    92

    87-97

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.066

    2.122

    2.094

    1.974

    1000

    1.574

    1.625

    1.600

    1.480

    500

    1.082

    1.113

    1.098

    0.978

    250

    0.643

    0.687

    0.665

    0.545

    125

    0.391

    0.406

    0.399

    0.279

    62.50

    0.256

    0.255

    0.256

    0.136

    31.25

    0.211

    0.221

    0.216

    0.096

    0

    0.119

    0.121

    0.120

    　

    LOD

    14.8pg/ml

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1.
  • 基因功能參考文獻：
    1. The results indicate that the IL-17B/IL-17RB signaling can promote the growth and migration of tumor cells. PMID: 27146881
    2. Our data revealed a new mechanism that IL-17B enhanced the progression of gastric cancer by activating mesenchymal stem cells. PMID: 28145881
    3. Segregation analysis revealed that variants c.475T>G in SKP1, c.671G>A in PROB1, and c.527G>A in IL17B in the 5q31.1-q35.3 linkage region, and c.850G>A in HKDC1 in the 10q22 locus completely segregated with the phenotype in the studied Keratoconus family PMID: 27703147
    4. This comprehensive review details the recognition of activity, signaling, and the roles of IL17B-IL17RB in breast cancer have caused to determination of new therapeutic mechanisms--{REVIEW} PMID: 28160754
    5. Studies indicate that interleukin 17 (IL-17B)/interleukin 17 receptor B (IL-17RB) signaling is essential for pancreatic cancer malignancy. PMID: 26285835
    6. IL-17B-IL-17RB signaling promotes pancreatic cancer malignancy. PMID: 25732306
    7. Results show that amplified signaling of interleukin-17 receptor B (IL-17RB) and its ligand IL-17B promot tumorigenicity in breast cancer cells and impeded acinus formation in immortalized normal mammary epithelial cells. PMID: 23851503
    8. IL-17B can enhance the effects of TNF-alpha on the production of cytokines and chemokines that control immune cell trafficking and neutrophil homeostasis in the inflamed tissues. PMID: 24056520
    9. IL-17A, IL-17B, IL-17F and IL-23 in systemic lupus erythematosus patients were examined and the correlation between levels of the investigated cytokines and VEGF, PIGF, as well as number of endothelial cells, was investigated. PMID: 23661335
    10. expression and functional study of IL-17B PMID: 22394632
    11. IL-17 may play an important role in the occurrence of nasal polyps by overexpression. PMID: 16805377

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-17 family
  • 組織特異性：
    Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
  • 數據庫鏈接：

    HGNC: 5982

    OMIM: 604627

    KEGG: hsa:27190

    STRING: 9606.ENSP00000261796

    UniGene: Hs.156979



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