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  4. Human Interleukin 4,IL-4 ELISA KIT

Human Interleukin 4,IL-4 ELISA KIT

  • 中文名稱：
    人白介素4(IL-4)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04633h
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E04633h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿.樣本中的IL4含量。IL4是一個在免疫學領域備受關注的靶點。它是一種細胞因子，在調節(jié)免疫反應、促進B細胞增殖和分化等方面發(fā)揮重要作用。研究機制主要圍繞IL4與其受體結合，激活下游信號通路，影響細胞的生長、分化及功能，有望為免疫相關疾病治療提供方向。試劑盒檢測范圍為31.25 pg/ml- 2000 pg/ml，靈敏度為7.8 pg/ml。適用于基礎醫(yī)學研究中Th1/Th2免疫平衡機制探索、炎癥模型動態(tài)監(jiān)測或藥物干預效果評價等場景，例如評估哮喘動物模型中的細胞因子表達譜，或篩選調節(jié)IL-4分泌的化合物。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    B cell growth factor 1 ELISA Kit; B cell IgG differentiation factor ELISA Kit; B Cell Stimulatory Factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BCGF 1 ELISA Kit; BCGF1 ELISA Kit; Binetrakin ELISA Kit; BSF-1 ELISA Kit; BSF1 ELISA Kit; IGG1 induction factor ELISA Kit; IL 4 ELISA Kit; IL-4 ELISA Kit; IL4 ELISA Kit; IL4_HUMAN ELISA Kit; Il4e12 ELISA Kit; Interleukin 4 ELISA Kit; Interleukin 4 variant 2 ELISA Kit; Interleukin 4; isoform 1 ELISA Kit; Interleukin-4 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit; MGC79402 ELISA Kit; Pitrakinra ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates.
  • 檢測范圍：
    31.25 pg/ml - 2000 pg/ml
  • 靈敏度：
    7.8 pg/ml
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    97

    Range %

    88-100

    1:2

    Average %

    95

    Range %

    92-101

    1:4

    Average %

    97

    Range %

    93-100

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of human IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    92

    90-95

    EDTA plasma (n=4)

    97

    92-101

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2000

    2.807

    2.634

    2.721

    2.549

    1000

    1.855

    1.767

    1.811

    1.639

    500

    0.964

    1.028

    0.996

    0.824

    250

    0.526

    0.529

    0.528

    0.356

    125

    0.375

    0.351

    0.363

    0.192

    62.5

    0.306

    0.293

    0.299

    0.128

    31.25

    0.215

    0.204

    0.209

    0.038

    0

    0.168

    0.175

    0.172

    　

  • 本試劑盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human IL-4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Squirt bottle, manifold dispenser, or automated microplate washer.
      • Absorbent paper for blotting the microtiter plate.
      • 100ml and 500ml graduated cylinders.
      • Deionized or distilled water.
      • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL and pipette tips.
      • Timer
      • Test tubes for dilution.

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 基因功能參考文獻：
    1. IL-33 has a role in the pathogenesis of autoimmune hepatitis (AIH) and affects the expression of IL-4, IL-17A, and of hypergammaglobulinemia PMID: 30034292
    2. Interleukin-4 induces a CD44high /CD49bhigh PC3 subpopulation with tumor-initiating characteristics. PMID: 29236307
    3. Allelic polymorphism C590T of the gene IL-4 is the most possible genetic marker of high predisposition to development of recurrent episodes of acute obstructive bronchitis in children. PMID: 30480406
    4. certain single nucleotide polymorphisms of IL4 gene could predispose individuals to recurrent aphthous stomatitis PMID: 29985726
    5. Germline variants in IL4 gene are associated with prostate cancer. PMID: 29298992
    6. The role of IL-4 in psoriasis. PMID: 28064550
    7. By establishing that IL-4 is posttranslationally regulated by TRX-promoted reduction of a disulfide bond, our findings highlight a novel regulatory mechanism of the type 2 immune response that is specific to IL-4 over IL-13. PMID: 30104382
    8. IL-4 polymorphisms might be associated with Kawasaki disease in an Iranian population. PMID: 28036156
    9. association between root resorption and IL4 gene polymorphisms was observed. PMID: 28617966
    10. these results demonstrated that variable number tandem repeat polymorphism in the IL-4 gene is associated with diabetic peripheral neuropathy in type 2 diabetes patients with coexisting cardiovascular disease PMID: 29182400
    11. serum antibodies against HP-NAP represent a state of risk, which is further exacerbated in IL-4 -590 T carriers. PMID: 27677314
    12. Polymorphisms at IL10 (-1082 G>A), IL4 (-589 C>T), CTLA4 (+49A>G), and DAO (+8956 C>G) genes were studied in 55 cases. PMID: 28750137
    13. Defective sirtuin-1 was found to increase IL-4 expression through acetylation of GATA-3 in patients with severe asthma compared with healthy controls. PMID: 26627546
    14. The results confirmed that the IL-4-590C/T polymorphism is correlated with the onset of RA and that carrying the T-allele can significantly increase the risk of rheumatoid arthritis in the Chinese Han population. PMID: 28975976
    15. the IL-4-590 C>T polymorphism does not influence the development of head and neck cancer. PMID: 29185028
    16. the present study suggests that IL-4 polymorphisms might play a role in susceptibility to inflammatory bowel disease and its major subtypes in the Iranian population PMID: 28872970
    17. IL-4R plays an important role in regulating hepatocellular carcinoma (HCC)cell survival and metastasis, and regulates the activity of the JAK1/STAT6 and JNK/ERK1/2 signaling pathways. We therefore suggest that IL-4/IL-4R may be a new therapeutic target for HCC PMID: 28665449
    18. IL-4 rs2227288 and IL-10 rs1800872 may contribute to an increased risk for virus-induced encephalitis. Through use of direct sequencing, we showed that genotypes of IL-4 rs2227288 and IL-10 rs1800872 may have particular host susceptibility to virus-induced encephalitis. IL-4 rs2227283 and IL-10 rs1800871 have no correlation in with risk of virus-induced encephalitis (both P>0.05) PMID: 28935853
    19. IL-4 and IL-8 genetic polymorphisms determine susceptibility to chronic Aggregatibacter actinomycetemcomitans periodontitis. PMID: 28859277
    20. The data indicate that USP4 interacts with and deubiquitinates IRF4, and also stabilizes IRF4 protein and promotes IRF4 function to facilitate IL-4 expression in Th2 cells, which may be related to the pathological process of rheumatic heart disease. PMID: 28791349
    21. Results show that higher levels of black carbon (BC) were associated with lower methylation of IL4 promoter CpG-48 5 days later and increased FeNO. The magnitude of association between BC exposure and demethylation of IL4 CpG-48 measured 5 days later appeared to be greater among seroatopic children, especially those sensitized to cockroach allergens. PMID: 28588744
    22. Association between the TT-genotype of IL-4 rs2070874 polymorphism and a severe phenotype of viral-induced wheeze further underlines the role IL-4 plays in the inflammation pathway leading to viral respiratory infections. PMID: 28950434
    23. the effects of IL4 gene polymorphisms on cancer risk may vary by cancer type and by ethnicity. PMID: 28656227
    24. these results showed that allergy responses further accelerated the IL-4-induced inhibition of tumor development through the activation of STAT6 pathways. PMID: 28587956
    25. we identified a subgroup of CVID patients with defective IL-4 signaling in T cells, with severe clinical features of inflammation and autoimmunity. PMID: 28476239
    26. The difference between allelic and genotypic frequencies of interleukin-4 (-590C/T) between patients and controls was not significant (p = 0.46). PMID: 29372577
    27. Data suggest that miRNA-340/429, which targeted IL-4, might be a potential approach for cancer treatment. PMID: 27895317
    28. this paper shows that expression of non-secreted IL-4 is associated with histone deacetylase inhibitor-induced cell death, histone acetylation and c-Jun regulation in gamma/delta T-cells PMID: 27556516
    29. Human CCL1 gene is selectively targeted by AhR in M(IL-4) macrophage. IL-4-induced epigenetic modification potentiates AhR-mediated CCL1 expression. PMID: 27888289
    30. Liver IL-4 mRNA is downregulated in patients with pancreatic cancer and cachexia. PMID: 27897439
    31. Low IL4 expression is associated with melanoma. PMID: 26993600
    32. In this review, we discuss the molecular mechanisms driving IL-4 production in Th2 and Tfh cells. PMID: 27072069
    33. In this review, we discuss the structural details of IL-4 and IL-4Ralpha subunit and the structural similarities between IL-4 and IL-13. We also describe detailed chemistry of type-I and type-II receptor complexes and their signaling pathways. Furthermore, we elaborate the strength of type-II hetero dimer signals in response to IL-4 and IL-13. PMID: 27165851
    34. IL-4 and IL-17 modulate the functional activity of phagocytes in the maternal blood, cord blood, and colostrum of diabetic mother. PMID: 29135055
    35. There was no significant difference in serum level of IL-4 between children with MPP (Mycoplasma pneumoniae pneumonia)and those with non-MPP. Among children with MPP, we found similar level of IL-4 regardless of the personal and family history of allergy and asthma or the presence of wheezing. PMID: 28057814
    36. The functional promoter polymorphisms IL4-590C/T and IL6-174G/C, which affect the IL-4 and IL-6 levels in north Indian subjects, were associated with kidney dysfunction and CKD PMID: 27996163
    37. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2. PMID: 29262348
    38. CGRP and IL-4 positively regulated APN/CD13 expression and activity in psoriatic fibroblasts. PMID: 28387421
    39. Results from these two large randomized aerobic exercise intervention trials suggest that aerobic exercise does not alter IL-10 or IL-4 in a manner consistent with chronic disease and cancer prevention. PMID: 27485297
    40. IL-4 signaling up-regulates the IL-25 axis in human monocytic cells, and IL-25 may provide autocrine signals in monocytes and macrophages to sustain IL-17Rb expression and predispose to alternative activation. PMID: 28421819
    41. IL4 VNTR B2 allele was only significantly associated with overall adiposity status before adjusting for ethnicity. PMID: 28293435
    42. Our results showed the role of IL4 in promoting breast cancer aggressiveness PMID: 28400477
    43. These data identified the IL-4/CXCL12 loop as a previously unrecognized pathway involved in lymphoid stroma polarization and as a potential therapeutic target in Follicular lymphoma patients. PMID: 28202459
    44. IL-4 genetic variations associated with susceptibility to or protection against chronic periodontitis are directly associated with influencing the response of immune cells to periodontopathogens PMID: 28114408
    45. in HIV/AIDS patients under antiretroviral therapy, IL-4 and IL-10 levels were significantly lower in lipodystrophy vs. non-lipodystrophy PMID: 28189545
    46. autologous CD4(+) T cells that are exposed to EVs from CD40/IL-4-stimulated CLL cells exhibit enhanced migration, immunological synapse signaling, and interactions with tumor cells. PMID: 27118451
    47. IL-4 substantially restores CD79b protein expression, sIgM expression, and BCR signaling. PMID: 27226435
    48. Tandem repeat polymorphisms of IL4 is associated with the severity of chronic periodontitis. PMID: 28053321
    49. Keratinocyte gene expression is critically shaped by IL-4, altering cell fate decisions, which are likely important for the clinical manifestations and pathology of allergic skin disease PMID: 27554818
    50. Inhibition of protein kinase C zeta expression in prostate cancer cells promoted chemotaxis of peripheral macrophages and acquisition of M2 phenotypic features. These results were further supported by the finding that silencing of endogenous protein kinase C zeta promoted the expression of prostate cancer cell-derived interleukin-4 and interleukin-10 PMID: 28631559

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  • 相關疾病：
    Ischemic stroke (ISCHSTR)
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6014

    OMIM: 147780

    KEGG: hsa:3565

    STRING: 9606.ENSP00000231449

    UniGene: Hs.73917



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