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Human Interleukin 3,IL-3 ELISA KIT

  • 中文名稱:
    人白介素3(IL-3)酶聯免疫試劑盒
  • 貨號:
    CSB-E04631h
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人白介素3(IL-3)酶聯免疫試劑盒(CSB-E04631h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿.樣本中的IL3含量。IL3是重要靶點。它是一種細胞因子,主要由活化T細胞等產生。在機體造血調控、免疫調節等方面起關鍵作用。研究圍繞其信號通路展開,旨在了解其在疾病發生發展機制,為開發靶向IL3的治療藥物,如單抗等提供依據。試劑盒檢測范圍為62.5 pg/ml-4000 pg/ml.,靈敏度為15.6 pg/ml.。適用于基礎醫學研究中細胞因子網絡調控機制探索、疾病動物模型中的IL-3水平動態監測、藥物干預效果評價等科研場景。為免疫學、腫瘤學、血液病學等領域的體外實驗研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Colony stimulating factor multiple ELISA Kit; Hematopoietic growth factor ELISA Kit; IL 3 ELISA Kit; IL-3 ELISA Kit; IL3 ELISA Kit; IL3_HUMAN ELISA Kit; Interleukin 3 (colony stimulating factor; multiple) ELISA Kit; Interleukin 3 ELISA Kit; Interleukin-3 ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; MGC79398 ELISA Kit; MGC79399 ELISA Kit; Multi CSF ELISA Kit; Multilineage colony stimulating factor ELISA Kit; Multipotential colony stimulating factor ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; OTTHUMP00000065963 ELISA Kit; P cell stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates.
  • 檢測范圍:
    62.5 pg/ml-4000 pg/ml.
  • 靈敏度:
    15.6 pg/ml.
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    93

    Range %

    86-102

    1:2

    Average %

    94

    Range %

    91-105

    1:4

    Average %

    96

    Range %

    90-102

    1:8

    Average %

    90

    Range %

    86-97

  • 回收率:

    The recovery of human IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    85-94

    EDTA plasma (n=4)

    91

    87-97

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    4000

    2.510

    2.634

    2.572

    2.422

    2000

    1.922

    1.867

    1.894

    1.745

    1000

    1.325

    1.228

    1.276

    1.127

    500

    0.874

    0.829

    0.852

    0.702

    250

    0.489

    0.481

    0.485

    0.336

    125

    0.343

    0.353

    0.348

    0.198

    62.5

    0.245

    0.234

    0.240

    0.090

    0

    0.154

    0.145

    0.150

     

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
  • 基因功能參考文獻:
    1. IL-3 may be involved in the immediate memory deficits in the chronic phase of schizophrenia. PMID: 29554544
    2. The present study demonstrates for the first time that IL-3 has an important role in enhancing the migration of human MSCs through regulation of the CXCR4/SDF-1alpha axis. These findings suggest a potential role of IL-3 in improving the efficacy of MSCs in regenerative cell therapy. PMID: 28705238
    3. These results indicate that IL-3 regulates endothelial cells-extracellular vesicles release, cargo and IL-3 angiogenic paracrine action via STAT5. PMID: 27157262
    4. T-GM-CSF and -IL-3 significantly, and reciprocally, blunted receptor binding and myeloid progenitor cell proliferation activity of both FL-GM-CSF and -IL-3 in vitro and in vivo PMID: 28344320
    5. Results show that IL-3 induces several signaling pathways associated with increased cell survival under oxidative stress. This activity correlates with previous fi ndings indicating glucose uptake stimulation by IL-3, which together contribute to an emerging picture of a broader mechanism promoting cell survival. PMID: 27862234
    6. The IL-3/ GM-CSF effected on the myofibroblastic differentiation of human adipose derive stromal cells (hASCs) as well as it did on human dermal fibroblasts (HDFs). PMID: 28377320
    7. Genetically engineered mesenchymal stromal cells produce IL-3 and TPO to further improve human scaffold-based xenograft models PMID: 28456746
    8. IL-3 hardly down-regulates the alpha-chain of its receptor without depleting the common beta-chain, which enables extraordinarily sustained signaling events, predominantly the activation of Stat5. PMID: 27443880
    9. genetic polymorphisms in the immune genes IL-3 rs181781 and CTLA4 rs4553808 may influence the TAC dose-adjusted concentrations PMID: 28112181
    10. Our results indicated that the IL-3 and IL-13 polymorphisms were not associated with rheumatoid arthritis (RA). stratification analyses suggested that the IL-13 rs1800925 CT and CT/CC genotypes increased the risk of RA in patients with erythrocyte sedimentation rate (ESR) <25.00. these findings suggest that the IL-13 rs1800925 C/T polymorphism may be associated with increased risk of RA in ESR PMID: 27323078
    11. results suggest that IL-3 and IL-12p40 could be considered as molecular predictors for recurrent wheezing due to RSV infection PMID: 26299549
    12. results suggest that IL3 is an important genetic regulator for human brain volume variation and implied that IL3 might have experienced weak or modest positive selection in the evolutionary history of humans PMID: 26875095
    13. Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent PMID: 25962901
    14. were not able to confirm the association of IL-3 SNPs with schizophrenia. PMID: 26277822
    15. findings showed that high plasma IL-3 levels are associated with high mortality in sepsis PMID: 25766237
    16. Genetic variation in interleukin-3 gene is associated with breast cancer risk. PMID: 24670917
    17. results suggest that HuR recognizes the ARE-rich region in the IL-3 3'-UTR and plays a role in the IL-3 3'-UTR-mediated post-transcriptional control in T-cells PMID: 24658545
    18. A single nucleotide polymorphism (SNP; rs20541) in the IL-13 gene has been recognized as a risk factor for asthma. PMID: 23978640
    19. This study is the first to link beta-catenin activation to IL-3 and suggests that targeting IL-3 signaling may be an effective approach for the inhibition of beta-catenin activity in some patients with AML. PMID: 24598054
    20. IL3 SNP rs40401 is significantly associated with the risk of asthma in young adult Japanese women. PMID: 24684517
    21. This is the first study to show a significant positive association between IL3 SNP rs40401 variant and the risk of rhinoconjunctivitis. PMID: 23953855
    22. IL3 rs2073506 G>A polymorphism is associated with an increased risk for esophageal cancer of nodal and metastatic stages. PMID: 23726808
    23. Genetic variation in the IL3 promoter affects human brain volume by regulating proliferation and survival of neural progenitors. PMID: 23226269
    24. Transgenic mice are used to study the developmental regulation of the closely linked IL-3 and granulocyte-macrophage colony stimulting factor (GM-CSF) locus and to identify DNA enhancer elements required for its correct activity in vivo. PMID: 23024272
    25. data indicate that within microenvironments rich in betac-family cytokines and TNF, eosinophils are a source of proMMP-9 and highlight a previously unrecognized role for synergistic interaction between TNF and betac-family cytokinea, for proMMP-9 synthesis PMID: 22321809
    26. This study demonistrated that Carriers of the minor allele for a single nucleotide polymorphism in IL13 (rs1295686) were more likely to report breast pain prior to surgery (P = .019). PMID: 22515947
    27. an IL-3 autocrine loop can drive a tumor endothelial switch and that targeting IL-3 might confer a significant therapeutic advantage to hamper tumor angiogenesis PMID: 21643009
    28. IL-3 provides cellular protection against amyloid-beta neurotoxicity in primary cortical neuronal cells and may play a neuroprotective role in Alzheimer's disease. PMID: 20964623
    29. The IL3 genotypes rs40401 and rs40401 were found to exert a protective effect against malaria attacks. PMID: 21224257
    30. Data indicate a role for PLCgamma2 and Ca(2+) signaling through the modulation of MEK/ERK in IL3/GM-csf stimulated human hematopoietic stem/progenitor cells. PMID: 21506110
    31. The interaction of 6-locus from the 5 interleukin genes might confer higher risk for graves'disease and Graves'ophthalmopathy than single risk allele. PMID: 20332709
    32. IL-3 genotypes of 60 acute rejection (AR) subjects and the 270 patients without AR demonstrated a significant relationship between genotype frequencies and the SNPs PMID: 21168724
    33. Human IL-3/GM-CSF knock-in mice support human alveolar macrophage development and human immune responses in the lung. PMID: 21262803
    34. propose that RhoH functions as a negative regulator for IL3-induced signals through modulation of the JAK-STAT pathway PMID: 20738848
    35. the domain 1 D-E loop disulfide of hbetac and beta(IL-3) in maintaining the precise positions of ligand-binding residues necessary for normal high affinity binding and signaling PMID: 20516062
    36. Two different modes of beta c binding are utilized in the presence of the hIL-3R alpha isoforms. PMID: 20472554
    37. a novel role for VPA in enhancing the potential of IL-3 to stimulate megakaryopoiesis as well as erythropoiesis PMID: 20381581
    38. The IL-3/GM-CSF locus undergoes progressive stages of activation, with stepwise increases in active modifications and the proportion of cytokine-expressing cells, throughout the course of T cell differentiation. PMID: 20147630
    39. IL-3 and oncogenic Abl regulate the myeloblast transcriptome by altering mRNA stability PMID: 19829692
    40. binding kinetics of native IL-3 and several variants to IL-3 receptor PMID: 11700046
    41. IL-3 induces MHC class II and B7.2 expression on eosinophils and renders them capable of supporting T cell proliferation to superantigen and antigen-derived peptides. PMID: 11714768
    42. inhibition of signaling by antisense oligodeoxynucleotides targeting the common beta chain of receptors PMID: 11763346
    43. ectopically expressed in myeloid leukemic cells with t(5;12)(q31;p13), suggesting that expression of IL3 was deregulated by the translocation, indicating a variant leukemogenic mechanism for translocations involving the 5' end of ETV6 PMID: 11861295
    44. Antiapoptotic cytokine IL-3 + SCF + FLT3L influence on proliferation of gamma-irradiated AC133+/CD34+ progenitor cells. PMID: 12002675
    45. Monocytes cultured in the presence of IL-3 (plus IL-4) differentiate into dendritic cells that produce less IL-12 and shift T helper (Th) cell responses toward a Th2 cytokine pattern. PMID: 12055233
    46. Data suggest that increased activity of mutated interleukin 3 is due to a change from a rare ligand to a common one, allowing the increase in IL-3-dependent signaling. PMID: 12093816
    47. role in potentiating hematopoietic cell migration PMID: 12135758
    48. The IL-3 gene is regulated by two enhancers that have distinct but overlapping tissue specificities. PMID: 12165512
    49. WEHI-3B-derived IL-3 stimulation of mcl-1 gene transcription through the SIE motif involves phosphorylation of PU.1 at serine 142 by a p38(MAPK)-dependent pathway. PMID: 12612065
    50. Incubation of eosinophils with IL-3 leads to reduced expression of IL-5R alpha which is sustained for up to 5 days; in contrast, expression of IL-3R alpha is increased by IL-3, whereas GM-CSF receptor alpha expression in eosinophils is unaffected by IL-3. PMID: 12759409

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  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    IL-3 family
  • 組織特異性:
    Activated T-cells, mast cells, natural killer cells.
  • 數據庫鏈接:

    HGNC: 6011

    OMIM: 147740

    KEGG: hsa:3562

    STRING: 9606.ENSP00000296870

    UniGene: Hs.694



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