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  4. Human Interferon-induced protein with tetratricopeptide repeats 1(IFIT1) ELISA kit

Human Interferon-induced protein with tetratricopeptide repeats 1(IFIT1) ELISA kit

  • 中文名稱：
    人具有四肽重復(fù)序列1的干擾素誘導(dǎo)蛋白(IFIT1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011018HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人具有四肽重復(fù)序列1的干擾素誘導(dǎo)蛋白(IFIT1)酶聯(lián)免疫試劑盒（CSB-EL011018HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的IFIT1含量。IFIT1，也稱為干擾素誘導(dǎo)蛋白與四肽重復(fù)1，是一種在干擾素處理后被誘導(dǎo)產(chǎn)生的蛋白質(zhì)，主要功能是抑制病毒復(fù)制和翻譯起始。其研究機(jī)制涉及病毒或干擾素調(diào)控，通過介導(dǎo)蛋白質(zhì)-蛋白質(zhì)相互作用和形成多蛋白質(zhì)復(fù)合物來執(zhí)行抗病毒免疫和干擾素信號(hào)通路的調(diào)控。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，該產(chǎn)品適用于病毒感染機(jī)制研究、宿主免疫應(yīng)答分析、炎癥性疾病模型建立及抗病毒藥物開發(fā)等科研場(chǎng)景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    C56 ELISA Kit; G10P1 ELISA Kit; GARG 16 ELISA Kit; GARG16 ELISA Kit; IFI 56 ELISA Kit; IFI 56K ELISA Kit; IFI-56K ELISA Kit; IFI56 ELISA Kit; IFIT-1 ELISA Kit; Ifit1 ELISA Kit; IFIT1_HUMAN ELISA Kit; IFNAI1 ELISA Kit; Interferon alpha inducible protein (MW 56kD) ELISA Kit; Interferon induced 56 kDa protein ELISA Kit; Interferon induced protein 56 ELISA Kit; Interferon inducible mRNA 561 ELISA Kit; Interferon-induced 56 kDa protein ELISA Kit; Interferon-induced protein with tetratricopeptide repeats 1 ELISA Kit; ISG56 ELISA Kit; OTTHUMP00000020062 ELISA Kit; P56 ELISA Kit; RNM561 ELISA Kit
  • 縮寫：
    IFIT1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFIT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 89-98
    1:2 Average % 98
    Range % 93-103
    1:4 Average % 88
    Range % 84-92
    1:8 Average % 102
    Range % 97-108
  • 回收率：
    The recovery of human IFIT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 96-105
    EDTA plasma (n=4) 89 83-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.835 2.986 2.911 2.770
    500 2.183 2.295 2.239 2.098
    250 1.321 1.397 1.359 1.218
    125 0.702 0.714 0.708 0.567
    62.5 0.453 0.435 0.444 0.303
    31.25 0.277 0.285 0.281 0.140
    15.6 0.229 0.224 0.227 0.086
    0 0.140 0.142 0.141 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses.
  • 基因功能參考文獻(xiàn)：
    1. Induction of IFIT1 in response to H9N2 virus infection or viral particle inoculation was more sensitive in HUVECs than in BEAS-2Bs. Data offers new insight into the innate immune response of endothelial cells to H9N2 virus infection. PMID: 29629559
    2. Human IFIT1 and mouse IFIT1B have divergent antiviral specificities: only IFIT1 proteins inhibit a virus encoding a cap 2'O-methyltransferase. PMID: 27240734
    3. Taken together, this study revealed that IFIT1 played an important antiviral role in human cytomegalovirus infected fetal astrocytes. PMID: 27589693
    4. Glioblastoma patients with high IFIT1 and low MGMT expression have an improved prognosis. PMID: 26980050
    5. IFIT1 significantly inhibited human parainfluenza virus type 3, whereas IFIT2, IFIT3, and IFIT5 were less effective or not at all. PMID: 27707917
    6. All the rubulaviruses tested were sensitive to the antiviral action of ISG56/IFIT1. PMID: 27512068
    7. IFIT1 is involved in the regulation of IFNalpha treatment for chronic hepatitis B and its polymorphism rs303218 can predict the end point virological response. PMID: 27956805
    8. IFIT1 is not a dominant restriction factor against three different families of negative-sense RNA viruses. PMID: 26157117
    9. Reovirus T3D infection induced STAT-1, ISG-15, IFIT-1, Mx1, and IFIT-3 expression. PMID: 25905045
    10. protein expression is inhibited by hepatitis C virus PMID: 23529855
    11. The specificity of IFIT1 for 2'O-unmethylated RNA serves as potent antiviral mechanism against viruses lacking 2'O-methyltransferase activity and at the same time allows unperturbed progression of the antiviral program in infected cells. PMID: 24098121
    12. TLR4 signaling, induced by LPS, increases the expression of melanoma differentiation-associated gene 5 (MDA5) and interferon PMID: 23684765
    13. ISG56 may play a role in immune and inflammatory reactions induced by toll-like receptor 3 signaling in human mesangial cells. PMID: 23363937
    14. Results indicate that miR-203 is an interferon-inducible miRNA that can negatively regulate a number of cellular mRNAs, including an interferon-stimulated gene target, IFIT1/ISG56, by destabilizing its mRNA transcript. PMID: 23785202
    15. Data suggested that PIV5 mRNAs are methylated at the 2'-hydroxyl group and that ISG56/IFIT1 inhibits the translation of PIV5 mRNA by some unrecognized mechanism. Also ISG56/IFIT1 is primarily responsible for the IFN-induced inhibition of PIV5. PMID: 23052390
    16. crystal structures of human IFIT5, its complex with PPP-RNAs, and an amino-terminal fragment of IFIT1 PMID: 23334420
    17. Hepatitis C virus infection suppresses the upregulation of a subset of effector molecules, including ISG56 and IFITM1. PMID: 21976647
    18. ISG56 interacts with ribosomal protein L15 in gastric cancer cells. PMID: 21612406
    19. Expression of ifi56 is enhanced significantly when the differentiation of APL cells was induced by all-trans retinoic acid. PMID: 21129252
    20. IFIT1 bound PPP-RNA antagonizes viruses by sequestering specific viral nucleic acids. PMID: 21642987
    21. The authors present evidence that P56 (ISG56 protein), the expression of which is strongly induced by IFN, double-stranded RNA and viruses, mediates the anti-HPV effect of IFN. PMID: 19008854
    22. ISG56 is a mediator of negative-feedback regulation of virus-triggered induction of type I IFNs and cellular antiviral responses PMID: 19416887

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    IFIT family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 5407

    OMIM: 147690

    KEGG: hsa:3434

    STRING: 9606.ENSP00000360869

    UniGene: Hs.20315



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