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  4. Human Interferon epsilon(IFNE) ELISA kit

Human Interferon epsilon(IFNE) ELISA kit

  • 中文名稱：
    人干擾素ε(IFNE)酶聯免疫試劑盒
  • 貨號：
    CSB-EL011049HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人干擾素ε(IFNE)酶聯免疫試劑盒（CSB-EL011049HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNE含量。IFNE（干擾素-ε）是一種免疫調節蛋白，具有抗病毒和抗菌作用。其研究機制涉及調節免疫細胞功能，增強免疫應答。IFNE在生物醫學中的應用包括病毒和細菌感染的治療，以及免疫相關疾病的預防和治療。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，該產品適用于科研領域對IFNE表達水平的定量分析，例如研究病毒感染模型中IFNE的動態變化、探索炎癥性疾病中黏膜免疫調節機制，或評估生殖系統相關疾病中IFNE的潛在生物標志物功能，為免疫學、病毒學及生殖醫學研究提供可靠的技術支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IFNE ELISA Kit; IFNE1 ELISA Kit; UNQ360/PRO655 ELISA Kit; Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %89
    Range %85-95
    1:2Average %93
    Range %88-99
    1:4Average %92
    Range %87-100
    1:8Average %95
    Range %88-99
  • 回收率：
    The recovery of human IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-100
    EDTA plasma (n=4)9388-97
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.058 2.011 2.035 1.931
    5001.664 1.547 1.606 1.502
    2501.188 1.149 1.169 1.065
    1250.739 0.704 0.722 0.618
    62.50.561 0.521 0.541 0.437
    31.20.375 0.354 0.365 0.261
    15.60.219 0.211 0.215 0.111
    00.105 0.103 0.104 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral and bacterial genital infections.
  • 基因功能參考文獻：
    1. IFN epsilon protects macrophages against HIV infection through a type I IFN independent mechanism. PMID: 27942584
    2. human IFNvarepsilon suppresses HIV replication at multiple stages of infection. PMID: 28045025
    3. female sex workers decreased susceptibility to HIV-1 infection also have increased levels of IFNE gene and protein expression in the cervical epithelium PMID: 26555708
    4. T Allele of nonsense polymorphism (rs2039381, Gln71Stop) of interferon-epsilon is a risk factor for the development of intracerebral hemorrhage. PMID: 24055696
    5. Full-length IFN-epsilon 5'UTR markedly suppressed mRNA expression under basal conditions. It contains 2 stable stem-loop structures which associate with importin 9. Loop 1 is essential for regulation of mRNA expression. PMID: 23851686
    6. genetic polymorphism is related to onset time of vitiligo in Korean patients PMID: 23802172
    7. Observational study of gene-disease association. (HuGE Navigator) PMID: 20574843
    8. Observational study of gene-disease association. (HuGE Navigator) PMID: 20588308
    9. Observational study of gene-disease association. (HuGE Navigator) PMID: 20601674
    10. The structue and mRNA expression pattern of IFN-epsilon1 suggest that it may have a function distinct from those other members of type I INF. PMID: 15233997
    11. TNF-alpha leads to stabilization of IFN-epsilon mRNA, increased IFN-epsilon synthesis, engagement of type I IFNRs, increased STAT1 expression and phosphorylation, and up-regulation of retinoic acid-inducible gene-I expression PMID: 17878351
    12. A meta-analysis and a single-nucleotide polymorphism (SNP) rs1333049 representing the 9p21.3 locus provide unprecedented evidence for association between genetic variants at chromosome 9p21.3 and risk of coronary artery disease. PMID: 18362232
    13. In macrophages IFN-tau increased the synthesis of 2',5'-oligoadenylate synthetase/RNase L, MxA protein, IL-10 & IL-6, but not of IL-1ss or TNF-alpha. PMID: 18842358

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 組織特異性：
    Endometrium-specific.
  • 數據庫鏈接：

    HGNC: 18163

    OMIM: 615223

    KEGG: hsa:338376

    STRING: 9606.ENSP00000418018

    UniGene: Hs.682604



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