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  4. Human Inter-alpha-trypsin inhibitor heavy chain H3(ITIH3) ELISA kit

Human Inter-alpha-trypsin inhibitor heavy chain H3(ITIH3) ELISA kit

  • 中文名稱：
    人間α胰蛋白酶抑制劑重鏈H3(ITIH3)酶聯免疫試劑盒
  • 貨號：
    CSB-EL011896HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人間α胰蛋白酶抑制劑重鏈H3(ITIH3)酶聯免疫試劑盒（CSB-EL011896HU）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ITIH3含量。ITIH3是一種在血清中作為透明質酸載體的蛋白質，同時促進與各種基質蛋白的相互作用。這些相互作用調節透明質酸的定位、合成和降解，對多種細胞活動至關重要。ITIH3在生理和病理過程中發揮重要作用，如炎癥、腫瘤發生、神經發育和精神疾病等。試劑盒檢測范圍為78.13 ng/mL-5000 ng/mL，該產品適用于基礎醫學研究中ITIH3蛋白表達水平分析，例如肝臟功能研究、炎癥相關疾病模型構建、生物標志物篩選等領域，可為探索ITIH3在細胞黏附、組織修復中的分子機制提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    ITIH3 ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H3 ELISA Kit; ITI heavy chain H3 ELISA Kit; ITI-HC3 ELISA Kit; Inter-alpha-inhibitor heavy chain 3 ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit
  • 縮寫：
    ITIH3
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78.13 ng/mL-5000 ng/mL
  • 靈敏度：
    19.53 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TIH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:20Average %92
    Range %86-99
    1:40Average %97
    Range %92-103
    1:80Average %86
    Range %80-89
    1:160Average %90
    Range %83-99
  • 回收率：
    The recovery of human TIH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9087-95
    EDTA plasma (n=4)9590-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2Average
    50000.083 0.085 0.084
    25000.139 0.147 0.143
    12500.223 0.220 0.222
    6250.329 0.347 0.338
    312.50.572 0.614 0.593
    156.30.814 0.849 0.832
    78.131.228 1.259 1.244
    02.081 2.226 2.154
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
  • 基因功能參考文獻：
    1. ITIH3 rs2535629 SNP was genotyped in N=256 patients receiving various antipsychotics for up to 26weeks. Study found no association of genotype with overall changes in Brief Psychiatric Rating Scale scores, but greater improvement of negative symptoms in minor allele carriers indicates that rs2535629 may help to identify a subset of schizophrenia patients with better treatment response to clozapine. PMID: 27396837
    2. confirmed the association of schizophrenia with ITIH3/4 in a Han Chinese population PMID: 26206863
    3. A novel association between suicide attempt and the ITIH3/4-region in a combined group of patients with bipolar disorder, schizophrenia and related psychosis spectrum disorders. PMID: 24461634
    4. Study showed the association of a polymorphism (rs2535629) of ITIH3 with psychiatric disorders in an Asian population and that rs2535629 influences the susceptibility to psychiatric disorders by affecting the expression level of GLT8D1 PMID: 24373612
    5. This study demonistrated that Genome-wide significant associations in schizophrenia to ITIH3/4 and extensive replication of associations reported by the Schizophrenia. PMID: 22614287
    6. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. PMID: 20515073
    7. ITIH3 single-nucleotide polymorphism is a novel genetic risk factor of myocardial infarction. PMID: 17211523
    8. Widespread heavy chain 3 was only present in urinary stone formers PMID: 17898697

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    ITIH family
  • 數據庫鏈接：

    HGNC: 6168

    OMIM: 146650

    KEGG: hsa:3699

    STRING: 9606.ENSP00000415769

    UniGene: Hs.76716



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