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  4. Human Insulin-like peptide INSL5(INSL5) ELISA kit

Human Insulin-like peptide INSL5(INSL5) ELISA kit

  • 中文名稱：
    人胰島素樣肽INSL5(INSL5)酶聯免疫試劑盒
  • 貨號：
    CSB-EL011749HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人胰島素樣肽INSL5(INSL5)酶聯免疫試劑盒（CSB-EL011749HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的INSL5含量。INSL5是一個靶點。它可能在人體生理功能調節等方面有重要作用。目前相關研究或許聚焦于其在細胞信號傳導、疾病發生發展機制等方面。雖然具體研究機制還需深入探索，但它有望成為開發針對特定疾病新療法的潛在靶點。試劑盒檢測范圍為8 ng/mL-140 ng/mL，該產品適用于科研領域對INSL5表達水平的分析，例如探究其在代謝性疾病、腸道功能或神經內分泌調控中的潛在作用機制，為體外實驗提供可靠的數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    INSL5 ELISA Kit; INSL5_HUMAN ELISA Kit; Insulin like peptide INSL5 ELISA Kit; Insulin-like peptide 5 ELISA Kit; Insulin-like peptide INSL5 A chain ELISA Kit; MGC126695 ELISA Kit; MGC126697 ELISA Kit; PRO182 ELISA Kit; RIF2 ELISA Kit; RP24-72J16.1 ELISA Kit; UNQ156 ELISA Kit; UNQ156/PRO182 ELISA Kit
  • 縮寫：
    INSL5
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    8 ng/mL-140 ng/mL
  • 靈敏度：
    5 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human INSL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 85-103
    1:2 Average % 96
    Range % 89-105
    1:4 Average % 90
    Range % 86-97
    1:8 Average % 93
    Range % 91-98
  • 回收率：
    The recovery of human INSL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 90-102
    EDTA plasma (n=4) 94 88-100
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    140 1.586 1.597 1.592
    80 0.943 0.955 0.949
    32 0.365 0.371 0.368
    16 0.175 0.184 0.180
    8 0.101 0.105 0.103
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor.
  • 基因功能參考文獻：
    1. Mass spectral analysis of purified enteroendocrine cells and tissue homogenates identified the exact sequence of A and B chains of INSL5 endogenously expressed in L-cells. PMID: 28857318
    2. analysis of the interaction mechanism of INSL5 with its receptor RXFP4 PMID: 28274616
    3. High serum INSL5 is associated with obesity in men. PMID: 27355242
    4. The C-terminus of the B-chain of human INSL5 is critical for cognate RXFP4 receptor activity. PMID: 26661035
    5. Insl5 is an orexigenic hormone released from colonic L-cells, which promotes appetite during conditions of energy deprivation. PMID: 25028498
    6. electrostatic interactions between INSL5 and RXFP4 PMID: 25043977
    7. High INSL5 expression is associated with enteroendocrine cells of the neuroendocrine tumors. PMID: 23128569
    8. INSL5 may be a unique marker of colorectal enteroendocrine cells, and INSL5-RXFP4 signaling might play a role in an autocrine/paracrine fashion in the colorectal epithelium and rectal neuroendocrine tumors. PMID: 23438439
    9. Insulin-like peptide 5 is an endogenous ligand for GPCR142 PMID: 15525639
    10. The hydrogen-bond network and electrostatic interactions between charged groups in INSL5 by NMR-monitored temperature and pH titrations was characterized. PMID: 19178384

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 組織特異性：
    Highly expressed in rectum with lower levels in uterus and ascending and descending colon.
  • 數據庫鏈接：

    HGNC: 6088

    OMIM: 606413

    KEGG: hsa:10022

    STRING: 9606.ENSP00000302724

    UniGene: Hs.251380



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