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  4. Human Inositol oxygenase(MIOX) ELISA kit

Human Inositol oxygenase(MIOX) ELISA kit

  • 中文名稱：
    人肌醇加氧酶(MIOX)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL013833HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人肌醇加氧酶(MIOX)酶聯(lián)免疫試劑盒（CSB-EL013833HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的MIOX含量。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于研究肌醇代謝相關(guān)疾病的分子機(jī)制、評估細(xì)胞或組織模型中MIOX表達(dá)調(diào)控，以及探索氧化應(yīng)激狀態(tài)下酶活性變化的科研實驗。實驗僅需100μL樣本即可完成檢測，配套提供標(biāo)準(zhǔn)曲線繪制與樣本濃度計算方法，滿足體外實驗中對微量樣本的高精度定量需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    0610009I10Rik ELISA Kit; AI314022 ELISA Kit; Aldehyde reductase-like 6 ELISA Kit; ALDRL6 ELISA Kit; C85427 ELISA Kit; Inositol oxygenase ELISA Kit; Kidney-specific protein 32 ELISA Kit; KSP32 ELISA Kit; MGC90217 ELISA Kit; MI oxygenase ELISA Kit; miox ELISA Kit; MIOX_HUMAN ELISA Kit; Myo-inositol oxygenase ELISA Kit; Renal-specific oxidoreductase ELISA Kit; RSOR ELISA Kit
  • 縮寫：
    MIOX
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    18.75 pg/mL-1200 pg/mL
  • 靈敏度：
    4.7 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human MIOX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 84-92
    1:2 Average % 102
    Range % 99-105
    1:4 Average % 86
    Range % 81-90
    1:8 Average % 98
    Range % 94-102
  • 回收率：
    The recovery of human MIOX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 89-100
    EDTA plasma (n=4) 91 88-95
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1200 2.443 2.378 2.411 2.217
    600 2.045 2.056 2.051 1.857
    300 1.577 1.398 1.488 1.294
    150 0.984 0.987 0.986 0.792
    75 0.641 0.638 0.640 0.446
    37.5 0.434 0.445 0.440 0.246
    18.75 0.334 0.329 0.332 0.138
    0 0.195 0.193 0.194 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 基因功能參考文獻(xiàn)：
    1. Our results suggest that serum MIOX levels do not change in polycystic ovary syndrome. It was, therefore, concluded that myoinositol deficiency observed in PCOS was not related to the level of MIOX enzyme which cleaves myoinositol. PMID: 29187000
    2. these findings highlight the epigenetic regulation of MIOX in the pathogenesis of diabetic tubulopathy. PMID: 28208054
    3. Data suggest that MIOX expression/activity is up-regulated in kidneys tubules during oxidative stress (as in obesity and diabetic nephropathy); up-regulation of MIOX activity is related to phosphorylation of serine/threonine residues. PMID: 26578517
    4. results demonstrated that the polymorphism (rs761745) in the promoter region of myo-inositol oxygenase(MIOX) gene may be associated with the development of type 1 diabetes mellitus in our studied population PMID: 19896870
    5. Molecular cloning and characterization of myo-inositol oxygenase as a 33kDa protein. PMID: 15504367
    6. expression of MIOX is up-regulated by a positive feedback mechanism where xylitol, one of the products of MI catabolism via the glucuronate-xylulose pathway, induces an overexpression of MIOX PMID: 15778219
    7. myo-inositol oxygenase (MIOX) PMID: 11716759

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Myo-inositol oxygenase family
  • 組織特異性：
    Kidney specific.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 14522

    OMIM: 606774

    KEGG: hsa:55586

    STRING: 9606.ENSP00000216075

    UniGene: Hs.129227



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