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  4. Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

Human Ig kappa chain V-IV region(IGKV4-1) ELISA kit

  • 中文名稱(chēng)：
    人Igκ鏈V-IV區(qū)(IGKV4-1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011443HU
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人Igκ鏈V-IV區(qū)(IGKV4-1)酶聯(lián)免疫試劑盒（CSB-EL011443HU）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物.樣本中的IGKV4-1含量。IGKV4 - 1 是重要的靶點(diǎn)。它在相關(guān)疾病的發(fā)生發(fā)展中可能發(fā)揮關(guān)鍵作用。研究機(jī)制方面，可能涉及到與免疫反應(yīng)、細(xì)胞信號(hào)傳導(dǎo)等過(guò)程的關(guān)聯(lián)，通過(guò)對(duì)其深入研究，有望揭示疾病的發(fā)病機(jī)制，為開(kāi)發(fā)針對(duì)性的治療方案奠定基礎(chǔ)。試劑盒檢測(cè)范圍為0.156 ng/ml-10 ng/ml，適用于科研領(lǐng)域中對(duì)B細(xì)胞功能、抗體生成機(jī)制及相關(guān)疾病模型的探索，例如免疫失調(diào)研究、抗體工程開(kāi)發(fā)或腫瘤微環(huán)境分析等場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    IGKV4-1Immunoglobulin kappa variable 4-1 ELISA Kit; Ig kappa chain V-IV region B17 ELISA Kit; Ig kappa chain V-IV region JI ELISA Kit; Ig kappa chain V-IV region Len ELISA Kit; Ig kappa chain V-IV region STH ELISA Kit
  • 縮寫(xiě)：
    IGKV4-1
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, cell lysates.
  • 檢測(cè)范圍：
    0.156 ng/ml -10 ng/ml
  • 靈敏度：
    0.039 ng/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線(xiàn)性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IGKV4-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    88

    Range %

    81-95

    1:2

    Average %

    96

    Range %

    89-103

    1:4

    Average %

    97

    Range %

    92-103

    1:8

    Average %

    93

    Range %

    86-100

  • 回收率：

    The recovery of human IGKV4-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    90-100

    EDTA plasma (n=4)

    98

    91-105

  • 標(biāo)準(zhǔn)曲線(xiàn)：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    10

    2.069

    2.034

    2.051

    1.922

    5

    1.347

    1.367

    1.357

    1.228

    2.5

    0.797

    0.828

    0.812

    0.683

    1.25

    0.428

    0.409

    0.419

    0.289

    0.625

    0.294

    0.311

    0.302

    0.173

    0.312

    0.235

    0.243

    0.239

    0.110

    0.156

    0.157

    0.164

    0.160

    0.031

    0

    0.134

    0.125

    0.130

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    V segment of the variable domain of immunoglobulins light chain that participates in the antigen recognition. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen.
  • 亞細(xì)胞定位：
    Secreted. Cell membrane.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 5834



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