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  4. Human Histone H4(HIST1H4A) ELISA kit

Human Histone H4(HIST1H4A) ELISA kit

  • 中文名稱：
    人組蛋白H4(HIST1H4A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL010429HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人組蛋白H4(HIST1H4A)酶聯(lián)免疫試劑盒（CSB-EL010429HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的HIST1H4A含量。HIST1H4A 是重要的組蛋白編碼基因，參與染色體結構和基因表達調控。在研究機制上，其表達異常與細胞增殖、分化及凋亡等過程相關，還可能與一些疾病發(fā)生發(fā)展存在聯(lián)系，或可成為疾病診斷和治療潛在靶點。試劑盒檢測范圍為37.5 pg/mL-2400 pg/mL，本試劑盒適用于體外科研場景中蛋白質相互作用研究、表觀遺傳學機制探索以及細胞分化/凋亡相關模型的分子水平分析，其寬泛的檢測范圍和多樣本兼容性為科研人員提供了靈活的實驗方案選擇，可滿足基礎醫(yī)學研究、分子生物學實驗等多種需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    dJ160A22.1 ELISA Kit; dJ160A22.2 ELISA Kit; dJ221C16.1 ELISA Kit; dJ221C16.9 ELISA Kit; FO108 ELISA Kit; H4 ELISA Kit; H4.k ELISA Kit; H4/a ELISA Kit; H4/b ELISA Kit; H4/c ELISA Kit; H4/d ELISA Kit; H4/e ELISA Kit; H4/g ELISA Kit; H4/h ELISA Kit; H4/I ELISA Kit; H4/j ELISA Kit; H4/k ELISA Kit; H4/m ELISA Kit; H4/n ELISA Kit; H4/p ELISA Kit; H4_HUMAN ELISA Kit; H4F2 ELISA Kit; H4F2iii ELISA Kit; H4F2iv ELISA Kit; H4FA ELISA Kit; H4FB ELISA Kit; H4FC ELISA Kit; H4FD ELISA Kit; H4FE ELISA Kit; H4FG ELISA Kit; H4FH ELISA Kit; H4FI ELISA Kit; H4FJ ELISA Kit; H4FK ELISA Kit; H4FM ELISA Kit; H4FN ELISA Kit; H4M ELISA Kit; HIST1H4A ELISA Kit; HIST1H4B ELISA Kit; HIST1H4C ELISA Kit; HIST1H4D ELISA Kit; HIST1H4E ELISA Kit; HIST1H4F ELISA Kit; HIST1H4H ELISA Kit; HIST1H4I ELISA Kit; HIST1H4J ELISA Kit; HIST1H4K ELISA Kit; HIST1H4L ELISA Kit; HIST2H4 ELISA Kit; HIST2H4A ELISA Kit; Hist4h4 ELISA Kit; Histone 1 H4a ELISA Kit; Histone 1 H4b ELISA Kit; Histone 1 H4c ELISA Kit; Histone 1 H4d ELISA Kit; Histone 1 H4e ELISA Kit; Histone 1 H4f ELISA Kit; Histone 1 H4h ELISA Kit; Histone 1 H4i ELISA Kit; Histone 1 H4j ELISA Kit; Histone 1 H4k ELISA Kit; Histone 1 H4l ELISA Kit; Histone 2 H4a ELISA Kit; histone 4 H4 ELISA Kit; Histone H4 ELISA Kit; MGC24116 ELISA Kit
  • 縮寫：
    HIST1H4A
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍：
    37.5 pg/mL-2400 pg/mL
  • 靈敏度：
    9.3 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human HIST1H4A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 101  
    Range % 95-108  
    1:2 Average % 88  
    Range % 83-99  
    1:4 Average % 89  
    Range % 87-92  
    1:8 Average % 110  
    Range % 106-115  
  • 回收率：
    The recovery of human HIST1H4A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2400 2.757 2.657 2.707 2.539  
    1200 2.024 1.924 1.974 1.806  
    600 1.226 1.146 1.186 1.018  
    300 0.665 0.655 0.660 0.492  
    150 0.390 0.380 0.385 0.217  
    75 0.281 0.271 0.276 0.108  
    37.5 0.225 0.215 0.220 0.052  
    0 0.173 0.163 0.168 　  
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

平均分:
4.0分 - 1 個評價

樣品類型：蛋白（重組/純化） 牛混合組蛋白，分別溶于PBS和血漿

樣品信息：牛

稀釋比：沒有稀釋

產(chǎn)品評價： 我用CSB-EL010429HU檢測牛混合組蛋白濃度，標準曲線用四參數(shù)模型擬合出來的R2為0.99771（詳見圖片），標準曲線符合預期且OD值在0至2.75范圍內。

By 陳老師

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 基因功能參考文獻：
    1. ata show that PP32 and SET/TAF-Ibeta proteins block HAT1-mediated H4 acetylation. PMID: 28977641
    2. Data suggest post-translational modifications of histones, trimethylation of lysine 36 in H3 (H3K36me3) and acetylation of lysine 16 in H4 (H4K16ac), have roles in DNA damage repair; H3K36me3 stimulates H4K16ac upon DNA double-strand break; SETD2, LEDGF, and KAT5 are required for these epigenetic changes. (SETD2 = SET domain containing 2; LEDGF = lens epithelium-derived growth factor; KAT5 = lysine acetyltransferase 5) PMID: 28546430
    3. Data show that Omomyc protein co-localized with proto-oncogene protein c-myc (c-Myc), protein arginine methyltransferase 5 (PRMT5) and histone H4 H4R3me2s-enriched chromatin domains. PMID: 26563484
    4. H4K12ac is regulated by estrogen receptor-alpha and is associated with BRD4 function and inducible transcription PMID: 25788266
    5. Systemic lupus erythematosus appears to be associated with an imbalance in histone acetyltransferases and histone deacetylase enzymes favoring pathologic H4 acetylation. PMID: 25611806
    6. Sumoylated human histone H4 prevents chromatin compaction by inhibiting long-range internucleosomal interactions. PMID: 25294883
    7. Acetylation at lysine 5 of histone H4 associated with lytic gene promoters during reactivation of Kaposi's sarcoma-associated herpesvirus. PMID: 25283865
    8. An increase in histone H4 acetylation caused by hypoxia in human neuroblastoma cell lines corresponds to increased levels of N-myc transcription factor in these cells. PMID: 24481548
    9. Data indicate that G1-phase histone assembly is restricted to CENP-A and H4. PMID: 23363600
    10. This study focused on the distribution of a specific histone modification, namely H4K12ac, in human sperm and characterized its specific enrichment sites in promoters throughout the whole human genome. PMID: 22894908
    11. SRP68/72 heterodimers as major nuclear proteins whose binding of histone H4 tail is inhibited by H4R3 methylation. PMID: 23048028
    12. TNF-alpha inhibition of AQP5 expression in human salivary gland acinar cells is due to the epigenetic mechanism by suppression of acetylation of histone H4. PMID: 21973049
    13. our data suggest that global histone H3 and H4 modification patterns are potential markers of tumor recurrence and disease-free survival in non-small cell lung cancer PMID: 22360506
    14. HAT1 differentially impacts nucleosome assembly of H3.1-H4 and H3.3-H4. PMID: 22228774
    15. phosphorylation of histone H4 Ser 47 catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4 PMID: 21724829
    16. the imatinib-induced hemoglobinization and erythroid differentiation in K562 cells are associated with global histone H4 PMID: 20949922
    17. Our findings reveal the molecular mechanisms whereby the DNA sequences within specific gene bodies are sufficient to nucleate the monomethylation of histone H4 lysine 200 which, in turn, reduces gene expression by half. PMID: 20512922
    18. Downregulated by zinc and upregulated by docosahexaenoate in a neuroblastoma cell line. PMID: 19747413
    19. low levels of histone acetylation is associated with the development and progression of gastric carcinomas, possibly through alteration of gene expression PMID: 12385581
    20. overexpression of MTA1 protein and acetylation level of histone H4 protein are closely related PMID: 15095300
    21. peptidylarginine deiminase 4 regulates histone Arg methylation by converting methyl-Arg to citrulline and releasing methylamine; data suggest that PAD4 mediates gene expression by regulating Arg methylation and citrullination in histones PMID: 15345777
    22. lack of biotinylation of K12 in histone H4 is an early signaling event in response to double-strand breaks PMID: 16177192
    23. incorporation of acetylated histone H4-K16 into nucleosomal arrays inhibits the formation of compact 30-nanometer-like fibers and impedes the ability of chromatin to form cross-fiber interactions PMID: 16469925
    24. Apoptosis is associated with global DNA hypomethylation and histone deacetylation events in leukemia cells. PMID: 16531610
    25. BTG2 contributes to retinoic acid activity by favoring differentiation through a gene-specific modification of histone H4 arginine methylation and acetylation levels. PMID: 16782888
    26. Relationship between histone H4 modification, epigenetic regulation of BDNF gene expression, and long-term memory for extinction of conditioned fear. PMID: 17522015
    27. H4 tail and its acetylation have novel roles in mediating recruitment of multiple regulatory factors that can change chromatin states for transcription regulation PMID: 17548343
    28. Brd2 bromodomain 2 is monomeric in solution and dynamically interacts with H4-AcK12; additional secondary elements in the long ZA loop may be a common characteristic of BET bromodomains. PMID: 17848202
    29. Spermatids Hypac-H4 impairment in mixed atrophy did not deteriorate further by AZFc region deletion. PMID: 18001726
    30. the SET8 and PCNA interaction couples H4-K20 methylation with DNA replication PMID: 18319261
    31. H4K20 monomethylation and PR-SET7 are important for L3MBTL1 function PMID: 18408754
    32. High expression of acetylated H4 is more common in aggressive than indolent cutaneous T-cell lymphoma. PMID: 18671804
    33. Our findings indicate an important role of histone H4 modifications in bronchial carcinogenesis PMID: 18974389
    34. results indicate, by acetylation of histone H4 K16 during S-phase, early replicating chromatin domains acquire the H4K16ac-K20me2 epigenetic label that persists on the chromatin throughout mitosis & is deacetylated in early G1-phase of the next cell cycle PMID: 19348949
    35. acetylated H4 is overexpressed in diffuse large B-cell lymphoma and peripheral T-cell lymphoma relative to normal lymphoid tissue. PMID: 19438744
    36. The release of histone H4 by holocrine secretion from the sebaceous gland may play an important role in innate immunity. PMID: 19536143
    37. histone modification including PRC2-mediated repressive histone marker H3K27me3 and active histone marker acH4 may involve in CD11b transcription during HL-60 leukemia cells reprogramming to terminal differentiation PMID: 19578722
    38. A role of Cdk7 in regulating elongation is further suggested by enhanced histone H4 acetylation and diminished histone H4 trimethylation on lysine 36-two marks of elongation-within genes when the kinase was inhibited. PMID: 19667075
    39. Data showed the dynamic fluctuation of histone H4 acetylation levels during mitosis, as well as acetylation changes in response to structurally distinct histone deacetylase inhibitors. PMID: 19805290
    40. Data directly implicate BBAP in the monoubiquitylation and additional posttranslational modification of histone H4 and an associated DNA damage response. PMID: 19818714

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  • 相關疾病：
    Chromosomal aberrations involving HISTONE H4 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;6)(q27;p21), with BCL6.
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H4 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4781

    OMIM: 142750

    KEGG: hsa:121504

    STRING: 9606.ENSP00000367034

    UniGene: Hs.143080



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