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Human HERV-K_7p22.1 provirus ancestral Env polyprotein(ERVK6) ELISA kit

  • 中文名稱:
    人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)酶聯免疫試劑盒
  • 貨號:
    CSB-EL007812HU
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)酶聯免疫試劑盒(CSB-EL007812HU)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的ERVK6含量。人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)是內源性逆轉錄病毒K組的一個成員,其Env蛋白在早期感染中參與受體識別和膜融合。ERVK6蛋白在血清、血漿和組織勻漿中定量測量,具有潛在的研究價值。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于體外樣本中ERVK6蛋白表達水平的精準分析,可為病毒進化研究、宿主基因組穩定性探索以及相關疾病模型的分子機制解析提供技術支持。該產品嚴格適用于科研場景,包括但不限于細胞實驗、動物模型或生物樣本庫中ERVK6蛋白的動態監測,為分子生物學及病毒學領域的研究者提供穩定可靠的檢測工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    ERVK-6 ELISA Kit; ERVK6Endogenous retrovirus group K member 6 Env polyprotein ELISA Kit; EnvK2 protein ELISA Kit; Envelope polyprotein ELISA Kit; HERV-K(C7) envelope protein ELISA Kit; HERV-K(HML-2.HOM) envelope protein ELISA Kit; HERV-K108 envelope protein ELISA Kit; HERV-K_7p22.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein ELISA Kit; SU); Transmembrane protein ELISA Kit; TM)] ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Homo sapiens (Human)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Others
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human ERVK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 95
    Range % 91-99
    1:2 Average % 103
    Range % 98-106
    1:4 Average % 110
    Range % 106-114
    1:8 Average % 92
    Range % 89-97
  • 回收率:
    The recovery of human ERVK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 84 81-89
    EDTA plasma (n=4) 97 93-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.251 2.301 2.276 2.154
    5 1.375 1.368 1.372 1.250
    2.5 0.782 0.778 0.780 0.658
    1.25 0.503 0.511 0.507 0.385
    0.625 0.325 0.336 0.331 0.209
    0.312 0.247 0.268 0.258 0.136
    0.156 0.192 0.186 0.189 0.067
    0 0.121 0.123 0.122  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型:血清

樣品信息:人

稀釋比:沒有稀釋

產品評價: 挺好的,靈敏度很高,精確度也高,實驗結果很好,是陽性數據,擬合曲線也挺好,試劑盒很好用,操作挺簡單的

By 肖老師

靶點詳情

  • 功能:
    Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties.; SU mediates receptor recognition.; TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.
  • 亞細胞定位:
    [Transmembrane protein]: Cell membrane; Single-pass type I membrane protein.; [Surface protein]: Cell membrane; Peripheral membrane protein.; [Endogenous retrovirus group K member 6 Env polyprotein]: Virion.
  • 蛋白家族:
    Beta type-B retroviral envelope protein family, HERV class-II K(HML-2) env subfamily
  • 組織特異性:
    Expressed in testis, and peripheral blood lymphocytes.
  • 數據庫鏈接:

    HGNC: 13915

    OMIM: 605626



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