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  4. Human Fibroblast growth factor 17(FGF17) ELISA kit

Human Fibroblast growth factor 17(FGF17) ELISA kit

  • 中文名稱：
    人成纖維細胞生長因子17(FGF17)酶聯免疫試劑盒
  • 貨號：
    CSB-EL008622HU
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    人成纖維細胞生長因子17(FGF17)酶聯免疫試劑盒（CSB-EL008622HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養上清液.樣本中的FGF17含量。FGF17是一種成纖維細胞生長因子，在胚胎發育等過程中發揮重要作用。其研究機制聚焦于它與受體結合后激活下游信號通路，調控細胞增殖、分化等。在腫瘤等疾病中可能參與發生發展，為相關治療提供潛在靶點。試劑盒檢測范圍為3.12 pg/ml-200 pg/ml.，研究者可將其應用于發育生物學、疾病機制研究或藥物干預實驗中FGF17表達水平的動態監測，為探索其生理功能及病理意義提供可靠的工具支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    FGF 13 ELISA Kit; FGF 17 ELISA Kit; FGF-17 ELISA Kit; FGF13 ELISA Kit; Fgf17 ELISA Kit; FGF17_HUMAN ELISA Kit; Fibroblast growth factor 17 ELISA Kit; HH20 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates.
  • 檢測范圍：
    3.12 pg/ml-200 pg/ml.
  • 靈敏度：
    0.78 pg/ml.
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human FGF17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    90

    Range %

    85-96

    1:2

    Average %

    95

    Range %

    88-101

    1:4

    Average %

    100

    Range %

    93-105

    1:8

    Average %

    93

    Range %

    86-101

  • 回收率：

    The recovery of human FGF17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    86-100

    EDTA plasma (n=4)

    97

    92-103

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    200

    2.264

    2.234

    2.249

    2.078

    100

    1.582

    1.667

    1.625

    1.454

    50

    0.986

    1.028

    1.007

    0.836

    25

    0.660

    0.629

    0.645

    0.474

    12.5

    0.453

    0.431

    0.442

    0.271

    6.25

    0.338

    0.343

    0.341

    0.170

    3.12

    0.251

    0.244

    0.247

    0.077

    0

    0.166

    0.175

    0.171

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development.
  • 基因功能參考文獻：
    1. FGF17 and IL17RD prpopsed as the two top candidates in the entire proteome on the basis of a statistical test of their protein-protein interaction patterns to proteins known to be altered in congenital hypogonadotropic hypogonadism. PMID: 23643382
    2. FGF17 expression is increased 2-fold in benign prostatic hyperplasia and may contribute to the increased epithelial proliferation seen in this disease. PMID: 15129425
  • 相關疾病：
    Hypogonadotropic hypogonadism 20 with or without anosmia (HH20)
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 組織特異性：
    Preferentially expressed in the embryonic brain.
  • 數據庫鏈接：

    HGNC: 3673

    OMIM: 603725

    KEGG: hsa:8822

    STRING: 9606.ENSP00000352414

    UniGene: Hs.248192



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